Mix-infection of S. Typhi and ParaTyphi A in Typhoid Fever and Chronic Typhoid Carriers: A Nested PCR Based Study in North India
Introduction: Enteric fever is a systemic disease caused by Salmonella organism such as serotypes Typhi and ParaTyphi A, B, C. Salmonella ParaTyphi A contributes more than 50% of all the enteric fever cases and it has recently been projected as an emerging pathogen. Materials and Methods: The p...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-11-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/5107/9167_CE(Ra)_F(Sh)_PF1(NJAK)_PFA(Sh).pdf |
| Summary: | Introduction: Enteric fever is a systemic disease caused by
Salmonella organism such as serotypes Typhi and ParaTyphi A,
B, C. Salmonella ParaTyphi A contributes more than 50% of all
the enteric fever cases and it has recently been projected as an
emerging pathogen.
Materials and Methods: The present study was aimed to
detect Salmonella Typhi and ParaTyphi A in urine, blood and stool
specimens collected from cases of enteric fever (110), chronic
typhoid carriers (46) and healthy controls (75) to explore the
possibility of mixed infection by nested PCR. A new nested PCR
primer was designed targeting putative fimbrial protein (stkG)
gene which is one of the fimbrial gene families to Salmonella
ParaTyphi A and for S. Typhi already reported primers targeting
flagellin (fliC) gene.
Results: Large volume of urine specimens (15 ml) was found
to be the best for detection of Salmonella serotypes. The
urine sample was found to have mixed-infection by both the
serotypes in 40.9% of the cases but lower in blood (27.3%) and
stool (13.6%).
Conclusion: The present study concludes that occurrence of
mixed infection may be quite frequent in typhoid and chronic
typhoid carriers’ individuals, although the reported recent rise
in ParaTyphi A incidence may not be real. |
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| ISSN: | 2249-782X 0973-709X |