Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation
Fat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acid...
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MDPI AG
2020-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/7/2446 |
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| author | Lulu Liu Yu Wang Xiaojuan Liang Xiao Wu Jiali Liu Shulin Yang Cong Tao Jin Zhang Jianhui Tian Jianguo Zhao Yanfang Wang |
| author_facet | Lulu Liu Yu Wang Xiaojuan Liang Xiao Wu Jiali Liu Shulin Yang Cong Tao Jin Zhang Jianhui Tian Jianguo Zhao Yanfang Wang |
| author_sort | Lulu Liu |
| collection | DOAJ |
| description | Fat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, is associated with fatty acid composition in pigs. As <i>SCD</i> was observed to be significantly induced in 3T3-L1 preadipocytes differentiation, we hypothesized that it plays a role in porcine adipocyte differentiation and fat deposition. In this study, we revealed that <i>SCD</i> is highly expressed in adipose tissues from seven-day-old piglets, compared to its expression in tissues from four-month-old adult pigs. Moreover, we found that <i>SCD</i> and lipogenesis-related genes were induced significantly in differentiated porcine adipocytes. Using CRISPR/Cas9 technology, we generated <i>SCD<sup>-/-</sup></i> porcine embryonic fibroblasts (PEFs) and found that the loss of <i>SCD</i> led to dramatically decreased transdifferentiation efficiency, as evidenced by the decreased expression of known lipid synthesis-related genes, lower levels of oil red O staining and significantly lower levels of triglyceride content. Our study demonstrates the critical role of <i>SCD</i> expression in porcine adipocyte differentiation and paves the way for identifying it as the promising candidate gene for less fat deposition in pigs. |
| first_indexed | 2024-03-10T20:44:35Z |
| format | Article |
| id | doaj.art-19a125e138e1471898c1e52bbcc524a0 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T20:44:35Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-19a125e138e1471898c1e52bbcc524a02023-11-19T20:25:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-04-01217244610.3390/ijms21072446Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte DifferentiationLulu Liu0Yu Wang1Xiaojuan Liang2Xiao Wu3Jiali Liu4Shulin Yang5Cong Tao6Jin Zhang7Jianhui Tian8Jianguo Zhao9Yanfang Wang10Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaBiotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaCollege of Biological, Chemical Sciences and Engineering, Jiaxing University, Jiaxing 314001, ChinaCollege of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaInstitute of Zoology, Chinese Academy of Sciences, Beijing 100101, ChinaLaboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, ChinaFat deposition, which influences pork production, meat quality and growth efficiency, is an economically important trait in pigs. Numerous studies have demonstrated that stearoyl-CoA desaturase (SCD), a key enzyme that catalyzes the conversion of saturated fatty acids into monounsaturated fatty acids, is associated with fatty acid composition in pigs. As <i>SCD</i> was observed to be significantly induced in 3T3-L1 preadipocytes differentiation, we hypothesized that it plays a role in porcine adipocyte differentiation and fat deposition. In this study, we revealed that <i>SCD</i> is highly expressed in adipose tissues from seven-day-old piglets, compared to its expression in tissues from four-month-old adult pigs. Moreover, we found that <i>SCD</i> and lipogenesis-related genes were induced significantly in differentiated porcine adipocytes. Using CRISPR/Cas9 technology, we generated <i>SCD<sup>-/-</sup></i> porcine embryonic fibroblasts (PEFs) and found that the loss of <i>SCD</i> led to dramatically decreased transdifferentiation efficiency, as evidenced by the decreased expression of known lipid synthesis-related genes, lower levels of oil red O staining and significantly lower levels of triglyceride content. Our study demonstrates the critical role of <i>SCD</i> expression in porcine adipocyte differentiation and paves the way for identifying it as the promising candidate gene for less fat deposition in pigs.https://www.mdpi.com/1422-0067/21/7/2446<i>SCD</i>adipocytestransdifferentiationadipogenesis |
| spellingShingle | Lulu Liu Yu Wang Xiaojuan Liang Xiao Wu Jiali Liu Shulin Yang Cong Tao Jin Zhang Jianhui Tian Jianguo Zhao Yanfang Wang Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation International Journal of Molecular Sciences <i>SCD</i> adipocytes transdifferentiation adipogenesis |
| title | Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation |
| title_full | Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation |
| title_fullStr | Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation |
| title_full_unstemmed | Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation |
| title_short | Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation |
| title_sort | stearoyl coa desaturase is essential for porcine adipocyte differentiation |
| topic | <i>SCD</i> adipocytes transdifferentiation adipogenesis |
| url | https://www.mdpi.com/1422-0067/21/7/2446 |
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