Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor
Early detection of <i>cis</i> phosphorylated tau (<i>cis</i> P-tau) may help as an effective treatment to control the progression of Alzheimer’s disease (AD). Recently, we introduced for the first time a monoclonal antibody (mAb) with high affinity against <i>cis</i&...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/879 |
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| author | Ayoub Shiravandi Farzaneh Yari Nahid Tofigh Mohammad Kazemi Ashtiani Koorosh Shahpasand Mohammad-Hossein Ghanian Faezeh Shekari Farnoush Faridbod |
| author_facet | Ayoub Shiravandi Farzaneh Yari Nahid Tofigh Mohammad Kazemi Ashtiani Koorosh Shahpasand Mohammad-Hossein Ghanian Faezeh Shekari Farnoush Faridbod |
| author_sort | Ayoub Shiravandi |
| collection | DOAJ |
| description | Early detection of <i>cis</i> phosphorylated tau (<i>cis</i> P-tau) may help as an effective treatment to control the progression of Alzheimer’s disease (AD). Recently, we introduced for the first time a monoclonal antibody (mAb) with high affinity against <i>cis</i> P-tau. In this study, the <i>cis</i> P-tau mAb was utilized to develop a label-free immunosensor. The antibody was immobilized onto a gold electrode and the electrochemical responses to the analyte were acquired by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). The immunosensor was capable of selective detection of <i>cis</i> P-tau among non-specific targets like <i>trans</i> P-tau and major plasma proteins. A wide concentration range (10 × 10<sup>−14</sup> M–3.0 × 10<sup>−9</sup> M) of <i>cis</i> P-tau was measured in PBS and human serum matrices with a limit of detection of 0.02 and 0.05 pM, respectively. Clinical applicability of the immunosensor was suggested by its long-term storage stability and successful detection of <i>cis</i> P-tau in real samples of cerebrospinal fluid (CSF) and blood serum collected from human patients at different stages of AD. These results suggest that this simple immunosensor may find great application in clinical settings for early detection of AD which is an unmet urgent need in today’s healthcare services. |
| first_indexed | 2024-03-09T20:35:38Z |
| format | Article |
| id | doaj.art-19aba719a22242d68056ed2ef4a145df |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:35:38Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-19aba719a22242d68056ed2ef4a145df2023-11-23T23:12:09ZengMDPI AGBiosensors2079-63742022-10-01121087910.3390/bios12100879Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical ImmunosensorAyoub Shiravandi0Farzaneh Yari1Nahid Tofigh2Mohammad Kazemi Ashtiani3Koorosh Shahpasand4Mohammad-Hossein Ghanian5Faezeh Shekari6Farnoush Faridbod7Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, IranCenter of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran P.O. Box 14155-6455, IranLaboratory of Neuro-Organic Chemistry, Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran 1417935840, IranDepartment of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, IranDepartment of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, IranDepartment of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, IranAdvanced Therapy Medicinal Product Technology Development Center, Royan Institute for Stem Cell Biology and Technology, Academic Center for Education, Culture and Research (ACECR), Tehran 1665659911, IranCenter of Excellence in Electrochemistry, School of Chemistry, College of Science, University of Tehran, Tehran P.O. Box 14155-6455, IranEarly detection of <i>cis</i> phosphorylated tau (<i>cis</i> P-tau) may help as an effective treatment to control the progression of Alzheimer’s disease (AD). Recently, we introduced for the first time a monoclonal antibody (mAb) with high affinity against <i>cis</i> P-tau. In this study, the <i>cis</i> P-tau mAb was utilized to develop a label-free immunosensor. The antibody was immobilized onto a gold electrode and the electrochemical responses to the analyte were acquired by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV). The immunosensor was capable of selective detection of <i>cis</i> P-tau among non-specific targets like <i>trans</i> P-tau and major plasma proteins. A wide concentration range (10 × 10<sup>−14</sup> M–3.0 × 10<sup>−9</sup> M) of <i>cis</i> P-tau was measured in PBS and human serum matrices with a limit of detection of 0.02 and 0.05 pM, respectively. Clinical applicability of the immunosensor was suggested by its long-term storage stability and successful detection of <i>cis</i> P-tau in real samples of cerebrospinal fluid (CSF) and blood serum collected from human patients at different stages of AD. These results suggest that this simple immunosensor may find great application in clinical settings for early detection of AD which is an unmet urgent need in today’s healthcare services.https://www.mdpi.com/2079-6374/12/10/879Alzheimer’s diseaseearly detectionantibody<i>cis</i> P-tauhuman serumelectrochemical biosensor |
| spellingShingle | Ayoub Shiravandi Farzaneh Yari Nahid Tofigh Mohammad Kazemi Ashtiani Koorosh Shahpasand Mohammad-Hossein Ghanian Faezeh Shekari Farnoush Faridbod Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor Biosensors Alzheimer’s disease early detection antibody <i>cis</i> P-tau human serum electrochemical biosensor |
| title | Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor |
| title_full | Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor |
| title_fullStr | Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor |
| title_full_unstemmed | Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor |
| title_short | Earlier Detection of Alzheimer’s Disease Based on a Novel Biomarker <em>cis</em> P-tau by a Label-Free Electrochemical Immunosensor |
| title_sort | earlier detection of alzheimer s disease based on a novel biomarker em cis em p tau by a label free electrochemical immunosensor |
| topic | Alzheimer’s disease early detection antibody <i>cis</i> P-tau human serum electrochemical biosensor |
| url | https://www.mdpi.com/2079-6374/12/10/879 |
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