Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico
Colorectal cancer (CRC) is a major global health challenge and one of the top 10 cancers in Mexico. Lifestyle and genetic factors influence CRC development, prognosis, and therapeutic response; identifying risk factors, such as the genes involved, is critical to understanding its behavior, mechanism...
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MDPI AG
2023-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/12/15/1941 |
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| author | Martha Patricia Gallegos-Arreola Asbiel Felipe Garibaldi-Ríos José Israel Cruz-Sánchez Luis Eduardo Figuera Carlos Alberto Ronquillo-Carreón Mónica Alejandra Rosales-Reynoso Belinda Claudia Gómez-Meda Irving Alejandro Carrillo-Dávila Ana María Puebla-Pérez Héctor Montoya-Fuentes Valeria Peralta-Leal Guillermo M. Zúñiga-González |
| author_facet | Martha Patricia Gallegos-Arreola Asbiel Felipe Garibaldi-Ríos José Israel Cruz-Sánchez Luis Eduardo Figuera Carlos Alberto Ronquillo-Carreón Mónica Alejandra Rosales-Reynoso Belinda Claudia Gómez-Meda Irving Alejandro Carrillo-Dávila Ana María Puebla-Pérez Héctor Montoya-Fuentes Valeria Peralta-Leal Guillermo M. Zúñiga-González |
| author_sort | Martha Patricia Gallegos-Arreola |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is a major global health challenge and one of the top 10 cancers in Mexico. Lifestyle and genetic factors influence CRC development, prognosis, and therapeutic response; identifying risk factors, such as the genes involved, is critical to understanding its behavior, mechanisms, and prognosis. The association between <i>KRAS</i> gene variants (rs8720 and rs12587) and CRC in the Mexican population was analyzed. We performed in silico analysis and analyzed 310 healthy individuals and 385 CRC patients using TaqMan assays and real-time PCR. The CC and GG genotypes of rs8720 and rs12587 were identified as CRC risk factors (<i>p</i> < 0.05). The CC and TC genotypes of the rs8720 were associated with rectal cancer, age over 50 years, moderately differentiated histology, and advanced cancer stage. TG and GG genotypes of the rs12587 variant were a risk factor in the CRC group, in patients with stage I–II, males, and stage III–IV non-chemotherapy response. The <i>TG</i> haplotype is protected against CRC. The combined CCGG genotype was linked to CRC risk. In silico analysis revealed that the rs12587 and rs8720 variants could influence KRAS gene regulation via miRNAs. In conclusion, rs8720 and rs12587 variants of the <i>KRAS</i> gene were associated with CRC risk and could influence <i>KRAS</i> regulation via miRNAs. |
| first_indexed | 2024-03-11T00:30:05Z |
| format | Article |
| id | doaj.art-19ac77dfbef844eb8f9a86db8a6fe8a6 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-11T00:30:05Z |
| publishDate | 2023-07-01 |
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| series | Cells |
| spelling | doaj.art-19ac77dfbef844eb8f9a86db8a6fe8a62023-11-18T22:44:42ZengMDPI AGCells2073-44092023-07-011215194110.3390/cells12151941Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In SilicoMartha Patricia Gallegos-Arreola0Asbiel Felipe Garibaldi-Ríos1José Israel Cruz-Sánchez2Luis Eduardo Figuera3Carlos Alberto Ronquillo-Carreón4Mónica Alejandra Rosales-Reynoso5Belinda Claudia Gómez-Meda6Irving Alejandro Carrillo-Dávila7Ana María Puebla-Pérez8Héctor Montoya-Fuentes9Valeria Peralta-Leal10Guillermo M. Zúñiga-González11División de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoEspecialidad en Oncología Médica, Universidad de Guadalajara (UdeG)/UMAE Hospital de Especialidades, Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoEspecialidad en Oncología Médica, Universidad de Guadalajara (UdeG)/UMAE Hospital de Especialidades, Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Jalisco, MexicoDepartamento de Biología Molecular y Genómica, Instituto de Genética Humana “Dr. Enrique Corona Rivera”, Centro Universitario de Ciencias de la Salud (CUCS), Universidad de Guadalajara (UdeG), Guadalajara 44340, Jalisco, MexicoDivisión de Genética, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Guadalajara 44340, Jalisco, MexicoLaboratorio de Inmunofarmacología, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara (UdeG), Guadalajara 44430, Jalisco, MexicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Jalisco, MexicoFacultad de Medicina e Ingeniería en Sistemas Computacionales de Matamoros, Universidad Autónoma de Tamaulipas, Ciudad Victoria 87300, Tamaulipas, MexicoDivisión de Medicina Molecular, Centro de Investigación Biomédica de Occidente (CIBO), Centro Médico Nacional de Occidente (CMNO), Instituto Mexicano del Seguro Social (IMSS), Sierra Mojada 800, Col. Independencia, Guadalajara 44340, Jalisco, MexicoColorectal cancer (CRC) is a major global health challenge and one of the top 10 cancers in Mexico. Lifestyle and genetic factors influence CRC development, prognosis, and therapeutic response; identifying risk factors, such as the genes involved, is critical to understanding its behavior, mechanisms, and prognosis. The association between <i>KRAS</i> gene variants (rs8720 and rs12587) and CRC in the Mexican population was analyzed. We performed in silico analysis and analyzed 310 healthy individuals and 385 CRC patients using TaqMan assays and real-time PCR. The CC and GG genotypes of rs8720 and rs12587 were identified as CRC risk factors (<i>p</i> < 0.05). The CC and TC genotypes of the rs8720 were associated with rectal cancer, age over 50 years, moderately differentiated histology, and advanced cancer stage. TG and GG genotypes of the rs12587 variant were a risk factor in the CRC group, in patients with stage I–II, males, and stage III–IV non-chemotherapy response. The <i>TG</i> haplotype is protected against CRC. The combined CCGG genotype was linked to CRC risk. In silico analysis revealed that the rs12587 and rs8720 variants could influence KRAS gene regulation via miRNAs. In conclusion, rs8720 and rs12587 variants of the <i>KRAS</i> gene were associated with CRC risk and could influence <i>KRAS</i> regulation via miRNAs.https://www.mdpi.com/2073-4409/12/15/1941colorectal neoplasms<i>KRAS</i> genepolymorphismsingle nucleotidein silico analysisgenetic variation |
| spellingShingle | Martha Patricia Gallegos-Arreola Asbiel Felipe Garibaldi-Ríos José Israel Cruz-Sánchez Luis Eduardo Figuera Carlos Alberto Ronquillo-Carreón Mónica Alejandra Rosales-Reynoso Belinda Claudia Gómez-Meda Irving Alejandro Carrillo-Dávila Ana María Puebla-Pérez Héctor Montoya-Fuentes Valeria Peralta-Leal Guillermo M. Zúñiga-González Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico Cells colorectal neoplasms <i>KRAS</i> gene polymorphism single nucleotide in silico analysis genetic variation |
| title | Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_full | Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_fullStr | Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_full_unstemmed | Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_short | Association of the rs8720 and rs12587 <i>KRAS</i> Gene Variants with Colorectal Cancer in a Mexican Population and Their Analysis In Silico |
| title_sort | association of the rs8720 and rs12587 i kras i gene variants with colorectal cancer in a mexican population and their analysis in silico |
| topic | colorectal neoplasms <i>KRAS</i> gene polymorphism single nucleotide in silico analysis genetic variation |
| url | https://www.mdpi.com/2073-4409/12/15/1941 |
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