β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice
This study aimed to investigate the effects of Lyophyllum decastes β-glucan (LDBG) in preventing high-fat diet (HFD)-induced obesity in male C57BL/6 mice, focusing on fecal gut microbiota (GM) and plasma metabolomics. LDBG treatment led to a reduction in body weight and an improvement in dyslipidemi...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-05-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624001683 |
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| author | Bin Sun Zhenzhen Huang Ying Xiao Fangfang Zhang Liang Pan Ling Yu Lin Ye Yanfang Liu Xiaodan Liu Qi Xu Jieying Liu Shiyue Yan |
| author_facet | Bin Sun Zhenzhen Huang Ying Xiao Fangfang Zhang Liang Pan Ling Yu Lin Ye Yanfang Liu Xiaodan Liu Qi Xu Jieying Liu Shiyue Yan |
| author_sort | Bin Sun |
| collection | DOAJ |
| description | This study aimed to investigate the effects of Lyophyllum decastes β-glucan (LDBG) in preventing high-fat diet (HFD)-induced obesity in male C57BL/6 mice, focusing on fecal gut microbiota (GM) and plasma metabolomics. LDBG treatment led to a reduction in body weight and an improvement in dyslipidemia in the HFD-fed mice. Additionally, LDBG decreased pro-inflammatory factors (TNF-α, IL-1β, and IL-6) in liver and abdominal adipose tissue and lowered lipopolysaccharide and leptin levels in plasma. LDBG also up-regulated mRNA levels of intestinal epithelial tight junction proteins, such as Occludin, Claudin-1, and zonula occludens-1 in colon tissue. Furthermore, 16S rRNA gene sequencing data suggested that LDBG reduced the relative abundance of Faecalibaculum and Ileibacterium while increasing that of Bifidobacterium and Oscillospiraceae. Untargeted metabolomics showed that LDBG significantly promoted Tryptophan metabolism, Glycerophospholipid metabolism, and the Citrate cycle, closely correlating with GM changes, particularly in Oscillospiraceae genera. This study demonstrates the potential of LDBG as a prebiotic in obesity prevention by regulating metabolite pathways. |
| first_indexed | 2024-04-24T11:21:03Z |
| format | Article |
| id | doaj.art-19ae5f584c56465c8b48920e6eb6de41 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2025-03-22T01:28:07Z |
| publishDate | 2024-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-19ae5f584c56465c8b48920e6eb6de412024-05-10T04:33:47ZengElsevierJournal of Functional Foods1756-46462024-05-01116106166β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese miceBin Sun0Zhenzhen Huang1Ying Xiao2Fangfang Zhang3Liang Pan4Ling Yu5Lin Ye6Yanfang Liu7Xiaodan Liu8Qi Xu9Jieying Liu10Shiyue Yan11School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaShanghai University of Traditional Chinese Medicine Laboratory Animal Center, Shanghai 201203, ChinaSchool of Health and Social Care, Shanghai Urban Construction Vocational College, Shanghai 201415, China; Corresponding authors.School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, ChinaSchool of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China; Corresponding authors.Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding authors.School of Health and Social Care, Shanghai Urban Construction Vocational College, Shanghai 201415, ChinaSchool of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaSchool of Public Health, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, ChinaThis study aimed to investigate the effects of Lyophyllum decastes β-glucan (LDBG) in preventing high-fat diet (HFD)-induced obesity in male C57BL/6 mice, focusing on fecal gut microbiota (GM) and plasma metabolomics. LDBG treatment led to a reduction in body weight and an improvement in dyslipidemia in the HFD-fed mice. Additionally, LDBG decreased pro-inflammatory factors (TNF-α, IL-1β, and IL-6) in liver and abdominal adipose tissue and lowered lipopolysaccharide and leptin levels in plasma. LDBG also up-regulated mRNA levels of intestinal epithelial tight junction proteins, such as Occludin, Claudin-1, and zonula occludens-1 in colon tissue. Furthermore, 16S rRNA gene sequencing data suggested that LDBG reduced the relative abundance of Faecalibaculum and Ileibacterium while increasing that of Bifidobacterium and Oscillospiraceae. Untargeted metabolomics showed that LDBG significantly promoted Tryptophan metabolism, Glycerophospholipid metabolism, and the Citrate cycle, closely correlating with GM changes, particularly in Oscillospiraceae genera. This study demonstrates the potential of LDBG as a prebiotic in obesity prevention by regulating metabolite pathways.http://www.sciencedirect.com/science/article/pii/S1756464624001683Lyophyllum decastes β-glucanGut microbiotaPlasma metabolomicDyslipidemiaObesity |
| spellingShingle | Bin Sun Zhenzhen Huang Ying Xiao Fangfang Zhang Liang Pan Ling Yu Lin Ye Yanfang Liu Xiaodan Liu Qi Xu Jieying Liu Shiyue Yan β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice Journal of Functional Foods Lyophyllum decastes β-glucan Gut microbiota Plasma metabolomic Dyslipidemia Obesity |
| title | β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice |
| title_full | β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice |
| title_fullStr | β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice |
| title_full_unstemmed | β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice |
| title_short | β-Glucan from Lyophyllum decastes regulates gut microbiota and plasma metabolites in high-fat diet-induced obese mice |
| title_sort | β glucan from lyophyllum decastes regulates gut microbiota and plasma metabolites in high fat diet induced obese mice |
| topic | Lyophyllum decastes β-glucan Gut microbiota Plasma metabolomic Dyslipidemia Obesity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624001683 |
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