Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia
Seventeen water samples were collected from four different thermal springs in Saudi Arabia. Microbiological assays were used to assess the antibacterial activities of bacterial colonies against antibiotic-resistant and susceptible-bacterial strains, and 16S rRNA gene sequencing was used to identify...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | Saudi Pharmaceutical Journal |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1319016423001330 |
| _version_ | 1797797176830590976 |
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| author | Omaish Alqahtani Paul Stapleton Simon Gibbons |
| author_facet | Omaish Alqahtani Paul Stapleton Simon Gibbons |
| author_sort | Omaish Alqahtani |
| collection | DOAJ |
| description | Seventeen water samples were collected from four different thermal springs in Saudi Arabia. Microbiological assays were used to assess the antibacterial activities of bacterial colonies against antibiotic-resistant and susceptible-bacterial strains, and 16S rRNA gene sequencing was used to identify the genus and species of these antibiotic-producing bacteria. Chromatography and spectroscopy were used to separate the active compounds and help figuring out what their structures were. Four compounds were isolated using bacteria: N-acetyltryptamine (1), isovaleric acid (2), ethyl-4-ethoxybenzoate (3) and phenylacetic acid (4). Compounds 1, 2 and 4 were produced from Bacillus pumilus and 3 was from Bacillus licheniformis (AH-E1). The outcomes of the minimum inhibitory concentrations (MICs) showed that all pure compounds produced in this work had antibacterial activities against Gram-positive pathogens (between 128 mg/L and 512 mg/L compared to the control) and compound 2 had activity against E. coli. |
| first_indexed | 2024-03-13T03:44:15Z |
| format | Article |
| id | doaj.art-19b02ea95ace4d589d062f19bb74527c |
| institution | Directory Open Access Journal |
| issn | 1319-0164 |
| language | English |
| last_indexed | 2024-03-13T03:44:15Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Saudi Pharmaceutical Journal |
| spelling | doaj.art-19b02ea95ace4d589d062f19bb74527c2023-06-23T04:42:36ZengElsevierSaudi Pharmaceutical Journal1319-01642023-07-0131712371243Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi ArabiaOmaish Alqahtani0Paul Stapleton1Simon Gibbons2Department of Pharmacognosy, College of Pharmacy, Najran University, Najran, Saudi Arabia; Research Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UK; Corresponding author at: College of Pharmacy, Najran University, Najran 1988, Saudi Arabia.Research Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London WC1N 1AX, UKCentre for Natural Products Discovery (CNPD), Liverpool John Moores University, Liverpool L3 3AF, England, UKSeventeen water samples were collected from four different thermal springs in Saudi Arabia. Microbiological assays were used to assess the antibacterial activities of bacterial colonies against antibiotic-resistant and susceptible-bacterial strains, and 16S rRNA gene sequencing was used to identify the genus and species of these antibiotic-producing bacteria. Chromatography and spectroscopy were used to separate the active compounds and help figuring out what their structures were. Four compounds were isolated using bacteria: N-acetyltryptamine (1), isovaleric acid (2), ethyl-4-ethoxybenzoate (3) and phenylacetic acid (4). Compounds 1, 2 and 4 were produced from Bacillus pumilus and 3 was from Bacillus licheniformis (AH-E1). The outcomes of the minimum inhibitory concentrations (MICs) showed that all pure compounds produced in this work had antibacterial activities against Gram-positive pathogens (between 128 mg/L and 512 mg/L compared to the control) and compound 2 had activity against E. coli.http://www.sciencedirect.com/science/article/pii/S1319016423001330Bacterial resistanceAntimicrobial agentsThermal springs16S rRNA gene sequencingBacillus spp. |
| spellingShingle | Omaish Alqahtani Paul Stapleton Simon Gibbons Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia Saudi Pharmaceutical Journal Bacterial resistance Antimicrobial agents Thermal springs 16S rRNA gene sequencing Bacillus spp. |
| title | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| title_full | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| title_fullStr | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| title_full_unstemmed | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| title_short | Production of antibacterial compounds using Bacillus spp. isolated from thermal springs in Saudi Arabia |
| title_sort | production of antibacterial compounds using bacillus spp isolated from thermal springs in saudi arabia |
| topic | Bacterial resistance Antimicrobial agents Thermal springs 16S rRNA gene sequencing Bacillus spp. |
| url | http://www.sciencedirect.com/science/article/pii/S1319016423001330 |
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