Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea
AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission el...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Press of International Journal of Ophthalmology (IJO PRESS)
2016-03-01
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| Series: | International Journal of Ophthalmology |
| Subjects: | |
| Online Access: | http://www.ijo.cn/en_publish/2016/3/20160301.pdf |
| _version_ | 1819232233758654464 |
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| author | Xian-Ning Liu Xiu-Ping Zhu Jie Wu Zheng-Jie Wu Yong Yin Xiang-Hua Xiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi |
| author_facet | Xian-Ning Liu Xiu-Ping Zhu Jie Wu Zheng-Jie Wu Yong Yin Xiang-Hua Xiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi |
| author_sort | Xian-Ning Liu |
| collection | DOAJ |
| description | AIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea.
METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype.
RESULTS: The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process. In vitro, the methyl thiazolyl tetrazolium results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes. The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation. The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer.
CONCLUSION: We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft. |
| first_indexed | 2024-12-23T11:57:36Z |
| format | Article |
| id | doaj.art-19b2840a191f4cf2bbe01d979d102b04 |
| institution | Directory Open Access Journal |
| issn | 2222-3959 2227-4898 |
| language | English |
| last_indexed | 2024-12-23T11:57:36Z |
| publishDate | 2016-03-01 |
| publisher | Press of International Journal of Ophthalmology (IJO PRESS) |
| record_format | Article |
| series | International Journal of Ophthalmology |
| spelling | doaj.art-19b2840a191f4cf2bbe01d979d102b042022-12-21T17:48:03ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982016-03-019332533110.18240/ijo.2016.03.01Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered corneaXian-Ning Liu0Xiu-Ping Zhu1Jie Wu2Zheng-Jie Wu3Yong Yin4Xiang-Hua Xiao5Xin Su6Bin Kong7Shi-Yin Pan8Hua Yang9Yan Cheng10Na An11Sheng-Li Mi12Shaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaXi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaBiomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaBiomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, ChinaBiomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaXi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaShaanxi Institute of Ophthalmology, Xi’an 710002, Shaanxi Province, China; Shaanxi Key Laboratory of Eye, Xi’an 710002, Shaanxi Province, China; Xi’an First Hospital, Xi’an 710002, Shaanxi Province, ChinaBiomanufacturing Engineering Laboratory, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, Guangdong Province, ChinaAIM: To assess acellular ostrich corneal matrix used as a scaffold to reconstruct a damaged cornea. METHODS: A hypertonic saline solution combined with a digestion method was used to decellularize the ostrich cornea. The microstructure of the acellular corneal matrix was observed by transmission electron microscopy (TEM) and hematoxylin and eosin (H&E) staining. The mechanical properties were detected by a rheometer and a tension machine. The acellular corneal matrix was also transplanted into a rabbit cornea and cytokeratin 3 was used to check the immune phenotype. RESULTS: The microstructure and mechanical properties of the ostrich cornea were well preserved after the decellularization process. In vitro, the methyl thiazolyl tetrazolium results revealed that extracts of the acellular ostrich corneas (AOCs) had no inhibitory effects on the proliferation of the corneal epithelial or endothelial cells or on the keratocytes. The rabbit lamellar keratoplasty showed that the transplanted AOCs were transparent and completely incorporated into the host cornea while corneal turbidity and graft dissolution occurred in the acellular porcine cornea (APC) transplantation. The phenotype of the reconstructed cornea was similar to a normal rabbit cornea with a high expression of cytokeratin 3 in the superficial epithelial cell layer. CONCLUSION: We first used AOCs as scaffolds to reconstruct damaged corneas. Compared with porcine corneas, the anatomical structures of ostrich corneas are closer to those of human corneas. In accordance with the principle that structure determines function, a xenograft lamellar keratoplasty also confirmed that the AOC transplantation generated a superior outcome compared to that of the APC graft.http://www.ijo.cn/en_publish/2016/3/20160301.pdfostrichacellular corneal stromatissue engineeringcornea |
| spellingShingle | Xian-Ning Liu Xiu-Ping Zhu Jie Wu Zheng-Jie Wu Yong Yin Xiang-Hua Xiao Xin Su Bin Kong Shi-Yin Pan Hua Yang Yan Cheng Na An Sheng-Li Mi Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea International Journal of Ophthalmology ostrich acellular corneal stroma tissue engineering cornea |
| title | Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea |
| title_full | Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea |
| title_fullStr | Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea |
| title_full_unstemmed | Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea |
| title_short | Acellular ostrich corneal stroma used as scaffold for construction of tissue-engineered cornea |
| title_sort | acellular ostrich corneal stroma used as scaffold for construction of tissue engineered cornea |
| topic | ostrich acellular corneal stroma tissue engineering cornea |
| url | http://www.ijo.cn/en_publish/2016/3/20160301.pdf |
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