EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY
Background: The World Health Organization admitted that the vaccination against Covid 19 limited the deaths, but not the spread of the disease. This requires a method allowing a specific, rapid and accurate diagnosis of the disease. We report a SPR assay that meets the requirements and can be appli...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
National Center of Infectious and Parasitic Diseases
2023-08-01
|
| Series: | Problems of Infectious and Parasitic Diseases |
| Subjects: | |
| Online Access: | https://pipd.ncipd.org/index.php/pipd/article/view/104 |
| _version_ | 1797389794957852672 |
|---|---|
| author | Petia Genova-Kalou Georgi Dyankov Hristo Kisov Vihar Mankov Evdokiya Hikova Stefka Krumova Nikola Malinowski |
| author_facet | Petia Genova-Kalou Georgi Dyankov Hristo Kisov Vihar Mankov Evdokiya Hikova Stefka Krumova Nikola Malinowski |
| author_sort | Petia Genova-Kalou |
| collection | DOAJ |
| description |
Background: The World Health Organization admitted that the vaccination against Covid 19 limited the deaths, but not the spread of the disease. This requires a method allowing a specific, rapid and accurate diagnosis of the disease. We report a SPR assay that meets the requirements and can be applied no only for SARS Cov-2 diagnosis but as a tool for early diagnosis of otherinfections.
Methods: Surface plasmon resonance (SPR) method was used to identify the binding of S/N protein to monoclonal antibodies. N-protein monoclonal antibody (NP mAb), S-protein monoclonal antibody (SP mAb), and receptor bind domain (RBD) antibody were used as recognition molecules. Ligands were deposited by the matrix-assisted laser evaporation (MAPLE) method, which guarantees maximum interaction specificity.
Results: We registered S/N protein binding to the corresponding mAbs and S protein to RBD antibody with high sensitivity: the interactions were observed at protein concentration about 130 femtomoles (fM). A very good specificity was observed: the measured S protein binding activity to NP mAb was below the limit of detection (LOD). The same was noticed for N protein binding to SP mAb.
Conclusions: The presented SPR assay possesses high sensitivity and selectivity and provides quantitative analysis. This makes it applicable for following the evolution of acute SARS-CoV-2 infection, especially at the early stages of viral replication which can be clinically useful.
|
| first_indexed | 2024-03-08T23:02:09Z |
| format | Article |
| id | doaj.art-19b6cc83af86497d9d4eee85e56b044b |
| institution | Directory Open Access Journal |
| issn | 0204-9155 2815-2808 |
| language | English |
| last_indexed | 2024-03-08T23:02:09Z |
| publishDate | 2023-08-01 |
| publisher | National Center of Infectious and Parasitic Diseases |
| record_format | Article |
| series | Problems of Infectious and Parasitic Diseases |
| spelling | doaj.art-19b6cc83af86497d9d4eee85e56b044b2023-12-15T15:50:38ZengNational Center of Infectious and Parasitic DiseasesProblems of Infectious and Parasitic Diseases0204-91552815-28082023-08-0150310.58395/peg69k16EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAYPetia Genova-Kalou0Georgi Dyankov1Hristo Kisov2Vihar Mankov3Evdokiya Hikova4Stefka Krumova5Nikola Malinowski6National Centre of Infectious and Parasitic DiseasesInstitute of Optical Materials and Technologies, Bulgarian Academy of Sciences, Sofia 1113, 109 Acad. G. Bonchev Str., BulgariaInstitute of Optical Materials and Technologies, Bulgarian Academy of Sciences, Sofia 1113, 109 Acad. G. Bonchev Str., BulgariaInstitute of Optical Materials and Technologies, Bulgarian Academy of Sciences, Sofia 1113, 109 Acad. G. Bonchev Str., BulgariaInstitute of Optical Materials and Technologies, Bulgarian Academy of Sciences, Sofia 1113, 109 Acad. G. Bonchev Str., BulgariaNational Center of Infectious and Parasitic Diseases, Department of Virology, 44A “Gen. Stoletov” Blvd., 1233 Sofia, BulgariaInstitute of Optical Materials and Technologies, Bulgarian Academy of Sciences, Sofia 1113, 109 Acad. G. Bonchev Str., Bulgaria Background: The World Health Organization admitted that the vaccination against Covid 19 limited the deaths, but not the spread of the disease. This requires a method allowing a specific, rapid and accurate diagnosis of the disease. We report a SPR assay that meets the requirements and can be applied no only for SARS Cov-2 diagnosis but as a tool for early diagnosis of otherinfections. Methods: Surface plasmon resonance (SPR) method was used to identify the binding of S/N protein to monoclonal antibodies. N-protein monoclonal antibody (NP mAb), S-protein monoclonal antibody (SP mAb), and receptor bind domain (RBD) antibody were used as recognition molecules. Ligands were deposited by the matrix-assisted laser evaporation (MAPLE) method, which guarantees maximum interaction specificity. Results: We registered S/N protein binding to the corresponding mAbs and S protein to RBD antibody with high sensitivity: the interactions were observed at protein concentration about 130 femtomoles (fM). A very good specificity was observed: the measured S protein binding activity to NP mAb was below the limit of detection (LOD). The same was noticed for N protein binding to SP mAb. Conclusions: The presented SPR assay possesses high sensitivity and selectivity and provides quantitative analysis. This makes it applicable for following the evolution of acute SARS-CoV-2 infection, especially at the early stages of viral replication which can be clinically useful. https://pipd.ncipd.org/index.php/pipd/article/view/104SARS-CoV-2spike (S-) proteinnucleocapsid (N-) proteinanti-SARS-CoV-1/2 antibodiesSurface Plasmon Resonance (SPR) assay |
| spellingShingle | Petia Genova-Kalou Georgi Dyankov Hristo Kisov Vihar Mankov Evdokiya Hikova Stefka Krumova Nikola Malinowski EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY Problems of Infectious and Parasitic Diseases SARS-CoV-2 spike (S-) protein nucleocapsid (N-) protein anti-SARS-CoV-1/2 antibodies Surface Plasmon Resonance (SPR) assay |
| title | EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY |
| title_full | EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY |
| title_fullStr | EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY |
| title_full_unstemmed | EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY |
| title_short | EVALUATION OF INTERACTIONS OF SARS-COV-2 STRUCTURAL PROTEINS WITH SPECIFIC ANTIBODIES BY SPR ASSAY |
| title_sort | evaluation of interactions of sars cov 2 structural proteins with specific antibodies by spr assay |
| topic | SARS-CoV-2 spike (S-) protein nucleocapsid (N-) protein anti-SARS-CoV-1/2 antibodies Surface Plasmon Resonance (SPR) assay |
| url | https://pipd.ncipd.org/index.php/pipd/article/view/104 |
| work_keys_str_mv | AT petiagenovakalou evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT georgidyankov evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT hristokisov evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT viharmankov evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT evdokiyahikova evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT stefkakrumova evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay AT nikolamalinowski evaluationofinteractionsofsarscov2structuralproteinswithspecificantibodiesbysprassay |