DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES
The influence of chosen saccharides on the growth and development of the in vitro shoot cultures of Paulownia tomentosa – valuable and multi-purpose tree species. The mainly sucrose (10–50 g dm-3), glucose and fructose (15.8 and 30 g dm-3) were tested in three subsequent passages. Then the usefulne...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Life Sciences in Lublin - Publishing House
2012-04-01
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| Series: | Acta Scientiarum Polonorum: Hortorum Cultus |
| Subjects: | |
| Online Access: | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3030 |
| _version_ | 1797393916380577792 |
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| author | Wojciech Litwińczuk Edyta Bochnia |
| author_facet | Wojciech Litwińczuk Edyta Bochnia |
| author_sort | Wojciech Litwińczuk |
| collection | DOAJ |
| description |
The influence of chosen saccharides on the growth and development of the in vitro shoot cultures of Paulownia tomentosa – valuable and multi-purpose tree species.
The mainly sucrose (10–50 g dm-3), glucose and fructose (15.8 and 30 g dm-3) were tested in three subsequent passages. Then the usefulness of saccharides in different applications of in vitro cultures was assessed. The basic MS medium supplemented with BA (1 mg dm-3), NAA (0.1 mg dm-3), and GA3 (0.1 mg dm-3) was used. Saccharides in tested concentrations influenced development of in vitro cultures. Proliferation of axillary shoots remained comparable whereas the development of adventitious shoots significantly and the frequency of vitrification decreased while the concentration of sucrose increased from 20 to 40 g dm-3. Glucose (15.8 g dm-3) stimulated both proliferation and elongation of axillary shoots. Sucrose (10 g dm-3), and fructose (15.8 g dm-3) favoured development of
adventitious shoots and vitrification of cultures. The media supplemented with sucrose (30–40 g dm-3) or glucose (15.8 g dm-3) may be recommended for micropropagation of true-to-type plants, whereas sucrose (50 g dm-3), and fructose (15.8 g dm-3) for long term storage, and plant breeding, respectively.
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| first_indexed | 2024-03-09T00:11:55Z |
| format | Article |
| id | doaj.art-19b7d05f57c042379b2215c27103fc01 |
| institution | Directory Open Access Journal |
| issn | 1644-0692 2545-1405 |
| language | English |
| last_indexed | 2024-03-09T00:11:55Z |
| publishDate | 2012-04-01 |
| publisher | University of Life Sciences in Lublin - Publishing House |
| record_format | Article |
| series | Acta Scientiarum Polonorum: Hortorum Cultus |
| spelling | doaj.art-19b7d05f57c042379b2215c27103fc012023-12-12T09:56:27ZengUniversity of Life Sciences in Lublin - Publishing HouseActa Scientiarum Polonorum: Hortorum Cultus1644-06922545-14052012-04-01112DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDESWojciech LitwińczukEdyta Bochnia The influence of chosen saccharides on the growth and development of the in vitro shoot cultures of Paulownia tomentosa – valuable and multi-purpose tree species. The mainly sucrose (10–50 g dm-3), glucose and fructose (15.8 and 30 g dm-3) were tested in three subsequent passages. Then the usefulness of saccharides in different applications of in vitro cultures was assessed. The basic MS medium supplemented with BA (1 mg dm-3), NAA (0.1 mg dm-3), and GA3 (0.1 mg dm-3) was used. Saccharides in tested concentrations influenced development of in vitro cultures. Proliferation of axillary shoots remained comparable whereas the development of adventitious shoots significantly and the frequency of vitrification decreased while the concentration of sucrose increased from 20 to 40 g dm-3. Glucose (15.8 g dm-3) stimulated both proliferation and elongation of axillary shoots. Sucrose (10 g dm-3), and fructose (15.8 g dm-3) favoured development of adventitious shoots and vitrification of cultures. The media supplemented with sucrose (30–40 g dm-3) or glucose (15.8 g dm-3) may be recommended for micropropagation of true-to-type plants, whereas sucrose (50 g dm-3), and fructose (15.8 g dm-3) for long term storage, and plant breeding, respectively. https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3030empress treemicropropagationaxillary shootsadventitious shootssucroseglucose |
| spellingShingle | Wojciech Litwińczuk Edyta Bochnia DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES Acta Scientiarum Polonorum: Hortorum Cultus empress tree micropropagation axillary shoots adventitious shoots sucrose glucose |
| title | DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES |
| title_full | DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES |
| title_fullStr | DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES |
| title_full_unstemmed | DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES |
| title_short | DEVELOPMENT OF ROYAL PAULOWNIA (Paulownia tomentosa Steud.) in vitro SHOOT CULTURES UNDER THE INFLUENCE OF DIFFERENT SACCHARIDES |
| title_sort | development of royal paulownia paulownia tomentosa steud in vitro shoot cultures under the influence of different saccharides |
| topic | empress tree micropropagation axillary shoots adventitious shoots sucrose glucose |
| url | https://czasopisma.up.lublin.pl/index.php/asphc/article/view/3030 |
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