Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence
<abstract language="eng">Toxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Química
2010-01-01
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| Series: | Química Nova |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400014 |
| _version_ | 1828530729549561856 |
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| author | Camila Aparecida Cotrim Saulo Santesso Garrido Eliane Trovatti Reinaldo Marchetto |
| author_facet | Camila Aparecida Cotrim Saulo Santesso Garrido Eliane Trovatti Reinaldo Marchetto |
| author_sort | Camila Aparecida Cotrim |
| collection | DOAJ |
| description | <abstract language="eng">Toxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11.7 kDa), preventing the cytotoxic activity of CcdB on the DNA gyrase. As an approach to understanding this interaction, CcdA41, a polypeptide derived from CcdA, was synthesized by solid-phase methodology and its interaction with CcdB was analyzed by steady state fluorescence. CcdA41 formed a stable complex with CcdBET2, a peptide based on CcdB, the more recently described bacterial topoisomerase inhibitor. |
| first_indexed | 2024-12-11T22:27:22Z |
| format | Article |
| id | doaj.art-19b95a89b652417d83dcc9177a41e6de |
| institution | Directory Open Access Journal |
| issn | 0100-4042 1678-7064 |
| language | English |
| last_indexed | 2024-12-11T22:27:22Z |
| publishDate | 2010-01-01 |
| publisher | Sociedade Brasileira de Química |
| record_format | Article |
| series | Química Nova |
| spelling | doaj.art-19b95a89b652417d83dcc9177a41e6de2022-12-22T00:48:14ZengSociedade Brasileira de QuímicaQuímica Nova0100-40421678-70642010-01-0133484184510.1590/S0100-40422010000400014Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescenceCamila Aparecida CotrimSaulo Santesso GarridoEliane TrovattiReinaldo Marchetto<abstract language="eng">Toxin-antitoxin (TA) systems contribute to plasmid stability by a mechanism called post-segregational killing. The ccd was the first TA system to be discovered with CcdB being the toxin and CcdA the antitoxin. CcdA, an 8.3 kDa protein, interacts with CcdB (11.7 kDa), preventing the cytotoxic activity of CcdB on the DNA gyrase. As an approach to understanding this interaction, CcdA41, a polypeptide derived from CcdA, was synthesized by solid-phase methodology and its interaction with CcdB was analyzed by steady state fluorescence. CcdA41 formed a stable complex with CcdBET2, a peptide based on CcdB, the more recently described bacterial topoisomerase inhibitor.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400014bacterial toxinpeptidesfluorescence |
| spellingShingle | Camila Aparecida Cotrim Saulo Santesso Garrido Eliane Trovatti Reinaldo Marchetto Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence Química Nova bacterial toxin peptides fluorescence |
| title | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence |
| title_full | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence |
| title_fullStr | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence |
| title_full_unstemmed | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence |
| title_short | Síntese, caracterização e estudos de interação de um análogo da antitoxina CcdA empregando fluorescência no estado estacionário Synthesis, characterization and interaction studies of an analog of CcdA antitoxin by steady state fluorescence |
| title_sort | sintese caracterizacao e estudos de interacao de um analogo da antitoxina ccda empregando fluorescencia no estado estacionario synthesis characterization and interaction studies of an analog of ccda antitoxin by steady state fluorescence |
| topic | bacterial toxin peptides fluorescence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422010000400014 |
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