Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i>
<i>Brassica napus</i> is a Cd hyperaccumulator, which is a serious threat to food and fodder safety. However, no related studies on developing Cd-safe <i>B. napus</i> have been reported yet. Here, we screened out a novel Cd uptake-related gene, <i>AtCUP1,</i> from...
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MDPI AG
2022-12-01
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| Online Access: | https://www.mdpi.com/2073-4409/11/23/3888 |
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| author | Junyu Yao Jiuyuan Bai Sha Liu Jingyan Fu Ying Zhang Tianshun Luo Hongpei Ren Rui Wang Yun Zhao |
| author_facet | Junyu Yao Jiuyuan Bai Sha Liu Jingyan Fu Ying Zhang Tianshun Luo Hongpei Ren Rui Wang Yun Zhao |
| author_sort | Junyu Yao |
| collection | DOAJ |
| description | <i>Brassica napus</i> is a Cd hyperaccumulator, which is a serious threat to food and fodder safety. However, no related studies on developing Cd-safe <i>B. napus</i> have been reported yet. Here, we screened out a novel Cd uptake-related gene, <i>AtCUP1,</i> from the major facilitator superfamily in <i>Arabidopsis thaliana</i>. The mutation of <i>AtCUP1</i> decreased Cd accumulation, both in roots and shoots of <i>A. thaliana</i>. Furthermore, the disruption of the <i>AtCUP1</i> gene by the CRISPR/Cas9 system significantly reduced Cd accumulation in <i>A. thaliana</i>. Interestingly, the disruption of the <i>BnCUP1</i> gene, an orthologous gene of <i>AtCUP1</i>, by the CRISPR/Cas9 system also diminished Cd accumulation in both roots and shoots of <i>B. napus</i> based on the hydroponics assay. Furthermore, for the field experiment, the Cd accumulations of <i>BnCUP1</i>-edited lines were reduced by 52% in roots and 77% in shoots compared to that of wild-type (WT) lines, and the biomass and yield of <i>BnCUP1</i>-edited lines increased by 42% and 47% of that of WT, respectively. Noteworthily, agronomic characteristics of <i>B. napus</i> were not apparently affected by <i>BnCUP1</i>-editing. Thus, <i>BnCUP1</i>-edited lines are excellent non-transgenic germplasm resources for reducing Cd accumulation without a distinct compromise in yield, which could be applied to agricultural production in Cd-contaminated soils. |
| first_indexed | 2024-03-09T17:50:04Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T17:50:04Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-19c2d7c2c5e344959aab42fdf09ee48c2023-11-24T10:45:30ZengMDPI AGCells2073-44092022-12-011123388810.3390/cells11233888Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i>Junyu Yao0Jiuyuan Bai1Sha Liu2Jingyan Fu3Ying Zhang4Tianshun Luo5Hongpei Ren6Rui Wang7Yun Zhao8Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, ChinaKey Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, China<i>Brassica napus</i> is a Cd hyperaccumulator, which is a serious threat to food and fodder safety. However, no related studies on developing Cd-safe <i>B. napus</i> have been reported yet. Here, we screened out a novel Cd uptake-related gene, <i>AtCUP1,</i> from the major facilitator superfamily in <i>Arabidopsis thaliana</i>. The mutation of <i>AtCUP1</i> decreased Cd accumulation, both in roots and shoots of <i>A. thaliana</i>. Furthermore, the disruption of the <i>AtCUP1</i> gene by the CRISPR/Cas9 system significantly reduced Cd accumulation in <i>A. thaliana</i>. Interestingly, the disruption of the <i>BnCUP1</i> gene, an orthologous gene of <i>AtCUP1</i>, by the CRISPR/Cas9 system also diminished Cd accumulation in both roots and shoots of <i>B. napus</i> based on the hydroponics assay. Furthermore, for the field experiment, the Cd accumulations of <i>BnCUP1</i>-edited lines were reduced by 52% in roots and 77% in shoots compared to that of wild-type (WT) lines, and the biomass and yield of <i>BnCUP1</i>-edited lines increased by 42% and 47% of that of WT, respectively. Noteworthily, agronomic characteristics of <i>B. napus</i> were not apparently affected by <i>BnCUP1</i>-editing. Thus, <i>BnCUP1</i>-edited lines are excellent non-transgenic germplasm resources for reducing Cd accumulation without a distinct compromise in yield, which could be applied to agricultural production in Cd-contaminated soils.https://www.mdpi.com/2073-4409/11/23/3888Cd-safe cropsmajor facilitator superfamilyCRISPR/Cas9agronomic traits |
| spellingShingle | Junyu Yao Jiuyuan Bai Sha Liu Jingyan Fu Ying Zhang Tianshun Luo Hongpei Ren Rui Wang Yun Zhao Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> Cells Cd-safe crops major facilitator superfamily CRISPR/Cas9 agronomic traits |
| title | Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> |
| title_full | Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> |
| title_fullStr | Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> |
| title_full_unstemmed | Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> |
| title_short | Editing of a Novel Cd Uptake-Related Gene <i>CUP1</i> Contributes to Reducing Cd Accumulations in <i>Arabidopsis thaliana</i> and <i>Brassica napus</i> |
| title_sort | editing of a novel cd uptake related gene i cup1 i contributes to reducing cd accumulations in i arabidopsis thaliana i and i brassica napus i |
| topic | Cd-safe crops major facilitator superfamily CRISPR/Cas9 agronomic traits |
| url | https://www.mdpi.com/2073-4409/11/23/3888 |
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