NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success
Background: RPL and RIF are challenges in reproductive medicine. The immune system plays a pivotal role in endometrial receptivity, successful implantation, and pregnancy complications. Immunological changes have been associated with RPL and RIF. Understanding immune dysregulation especially in NK a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/12/17/5585 |
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| author | Anne-Sophie Braun Kilian Vomstein Elisabeth Reiser Susanne Tollinger Christiana Kyvelidou Katharina Feil Bettina Toth |
| author_facet | Anne-Sophie Braun Kilian Vomstein Elisabeth Reiser Susanne Tollinger Christiana Kyvelidou Katharina Feil Bettina Toth |
| author_sort | Anne-Sophie Braun |
| collection | DOAJ |
| description | Background: RPL and RIF are challenges in reproductive medicine. The immune system plays a pivotal role in endometrial receptivity, successful implantation, and pregnancy complications. Immunological changes have been associated with RPL and RIF. Understanding immune dysregulation especially in NK and T cell subtypes may lead to better diagnostic concepts and treatments. From July 2019 to August 2020 patients with RPL and RIF underwent a standardized diagnostic procedure including endometrial biopsies. Immune cell analysis was performed using flow cytometry. Patients were contacted in March 2023 and interviewed concerning their pregnancy outcomes following diagnostics. Results: Out of 68 patients undergoing endometrial biopsies, 49 patients were finally included. Live birth rates were high with 72% in RPL and 86% in RIF. Immune cell analysis revealed that patients with RPL had more cytotoxic CD56<sup>dim</sup>CD16<sup>high</sup> cells, while RIF patients had more CD56<sup>+</sup> uNK cells. RPL patients with pregnancy complications showed increased NKT cell percentages. Conclusion: Our findings suggest specific immune changes in RPL and RIF patients, offering potential therapeutic targets. Tailored immunotherapy based on endometrial immunophenotyping might be an option, but further research is needed. |
| first_indexed | 2024-03-10T23:19:12Z |
| format | Article |
| id | doaj.art-19c37b2bc0ba410085bb736247eb9cfa |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T23:19:12Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-19c37b2bc0ba410085bb736247eb9cfa2023-11-19T08:22:40ZengMDPI AGJournal of Clinical Medicine2077-03832023-08-011217558510.3390/jcm12175585NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy SuccessAnne-Sophie Braun0Kilian Vomstein1Elisabeth Reiser2Susanne Tollinger3Christiana Kyvelidou4Katharina Feil5Bettina Toth6Department of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaDepartment of Gynecological Endocrinology and Reproductive Medicine, Medical University of Innsbruck, Anichstraße 35, 6020 Innsbruck, AustriaBackground: RPL and RIF are challenges in reproductive medicine. The immune system plays a pivotal role in endometrial receptivity, successful implantation, and pregnancy complications. Immunological changes have been associated with RPL and RIF. Understanding immune dysregulation especially in NK and T cell subtypes may lead to better diagnostic concepts and treatments. From July 2019 to August 2020 patients with RPL and RIF underwent a standardized diagnostic procedure including endometrial biopsies. Immune cell analysis was performed using flow cytometry. Patients were contacted in March 2023 and interviewed concerning their pregnancy outcomes following diagnostics. Results: Out of 68 patients undergoing endometrial biopsies, 49 patients were finally included. Live birth rates were high with 72% in RPL and 86% in RIF. Immune cell analysis revealed that patients with RPL had more cytotoxic CD56<sup>dim</sup>CD16<sup>high</sup> cells, while RIF patients had more CD56<sup>+</sup> uNK cells. RPL patients with pregnancy complications showed increased NKT cell percentages. Conclusion: Our findings suggest specific immune changes in RPL and RIF patients, offering potential therapeutic targets. Tailored immunotherapy based on endometrial immunophenotyping might be an option, but further research is needed.https://www.mdpi.com/2077-0383/12/17/5585reproductive immunologyuterine NK cellsimmunophenotypeendometriummiscarriage |
| spellingShingle | Anne-Sophie Braun Kilian Vomstein Elisabeth Reiser Susanne Tollinger Christiana Kyvelidou Katharina Feil Bettina Toth NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success Journal of Clinical Medicine reproductive immunology uterine NK cells immunophenotype endometrium miscarriage |
| title | NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success |
| title_full | NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success |
| title_fullStr | NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success |
| title_full_unstemmed | NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success |
| title_short | NK and T Cell Subtypes in the Endometrium of Patients with Recurrent Pregnancy Loss and Recurrent Implantation Failure: Implications for Pregnancy Success |
| title_sort | nk and t cell subtypes in the endometrium of patients with recurrent pregnancy loss and recurrent implantation failure implications for pregnancy success |
| topic | reproductive immunology uterine NK cells immunophenotype endometrium miscarriage |
| url | https://www.mdpi.com/2077-0383/12/17/5585 |
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