Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i>
Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a serious threat to global public health due to its capacity of tolerate conventional antibiotics. Medicinal plants are traditionally used to treat infectious diseases caused by bacterial pathogens. In the present study, 16 med...
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MDPI AG
2022-11-01
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| author | In-Geun Jung Jae-Young Jeong Seung-Hoon Yum You-Jin Hwang |
| author_facet | In-Geun Jung Jae-Young Jeong Seung-Hoon Yum You-Jin Hwang |
| author_sort | In-Geun Jung |
| collection | DOAJ |
| description | Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a serious threat to global public health due to its capacity of tolerate conventional antibiotics. Medicinal plants are traditionally used to treat infectious diseases caused by bacterial pathogens. In the present study, 16 medicinal plants were screened for antibacterial activities to preselect more effective species. Ethanol extracts of selected medicinal plants (<i>Caesalpinia sappan</i> L., <i>Glycyrrhiza uralensis</i> Fisch., <i>Sanguisorba officinalis</i> L., and <i>Uncaria gambir</i> Roxb) were partitioned successively with different solvents (n-hexane, chloroform, ethyl acetate, 1-butanol, and water). Disc diffusion assay and broth microdilution were performed to evaluate the antibacterial activities of plant extracts and fractions against <i>Staphylococcus aureus</i> strains. Furthermore, the cytotoxicity of the extracts and fractions was determined against the human hepatoma (HepG2) and human lung carcinoma (A549) cell lines using a trypan blue exclusion method. A few extracts and fractions showed significant inhibitory effects on the bacterial growth of all tested strains, including multidrug-resistance (MDR) clinical isolates. The ethyl acetate fraction of <i>C. sappan</i> had the most potent effects with minimum inhibitory/bactericidal concentrations (MIC/MBC) of 31.2/62.5 μg/mL and showed low cytotoxicity with over 90% cell viability in both cells. Our results suggest that medicinal plants have considerable potential as alternatives to conventional antibiotics. |
| first_indexed | 2024-03-09T18:07:38Z |
| format | Article |
| id | doaj.art-19c55ea040a841e4a9db7a181e75ec3b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:07:38Z |
| publishDate | 2022-11-01 |
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| record_format | Article |
| series | Molecules |
| spelling | doaj.art-19c55ea040a841e4a9db7a181e75ec3b2023-11-24T09:21:06ZengMDPI AGMolecules1420-30492022-11-012722778010.3390/molecules27227780Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i>In-Geun Jung0Jae-Young Jeong1Seung-Hoon Yum2You-Jin Hwang3Department of Biomedical Engineering, College of Health Science, Gachon University, Incheon 21936, KoreaDepartment of Health Sciences & Technology, Gachon Advanced Institute for Health Sciences & Technology, Gachon University, Incheon 21999, KoreaDepartment of Biomedical Engineering, College of Health Science, Gachon University, Incheon 21936, KoreaDepartment of Biomedical Engineering, College of Health Science, Gachon University, Incheon 21936, KoreaMethicillin-resistant <i>Staphylococcus aureus</i> (MRSA) is a serious threat to global public health due to its capacity of tolerate conventional antibiotics. Medicinal plants are traditionally used to treat infectious diseases caused by bacterial pathogens. In the present study, 16 medicinal plants were screened for antibacterial activities to preselect more effective species. Ethanol extracts of selected medicinal plants (<i>Caesalpinia sappan</i> L., <i>Glycyrrhiza uralensis</i> Fisch., <i>Sanguisorba officinalis</i> L., and <i>Uncaria gambir</i> Roxb) were partitioned successively with different solvents (n-hexane, chloroform, ethyl acetate, 1-butanol, and water). Disc diffusion assay and broth microdilution were performed to evaluate the antibacterial activities of plant extracts and fractions against <i>Staphylococcus aureus</i> strains. Furthermore, the cytotoxicity of the extracts and fractions was determined against the human hepatoma (HepG2) and human lung carcinoma (A549) cell lines using a trypan blue exclusion method. A few extracts and fractions showed significant inhibitory effects on the bacterial growth of all tested strains, including multidrug-resistance (MDR) clinical isolates. The ethyl acetate fraction of <i>C. sappan</i> had the most potent effects with minimum inhibitory/bactericidal concentrations (MIC/MBC) of 31.2/62.5 μg/mL and showed low cytotoxicity with over 90% cell viability in both cells. Our results suggest that medicinal plants have considerable potential as alternatives to conventional antibiotics.https://www.mdpi.com/1420-3049/27/22/7780antibacterialcytotoxicitymedicinal plantsmultidrug-resistancemethicillin-resistant <i>Staphylococcus aureus</i> |
| spellingShingle | In-Geun Jung Jae-Young Jeong Seung-Hoon Yum You-Jin Hwang Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> Molecules antibacterial cytotoxicity medicinal plants multidrug-resistance methicillin-resistant <i>Staphylococcus aureus</i> |
| title | Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full | Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_fullStr | Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_short | Inhibitory Effects of Selected Medicinal Plants on Bacterial Growth of Methicillin-Resistant <i>Staphylococcus aureus</i> |
| title_sort | inhibitory effects of selected medicinal plants on bacterial growth of methicillin resistant i staphylococcus aureus i |
| topic | antibacterial cytotoxicity medicinal plants multidrug-resistance methicillin-resistant <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/1420-3049/27/22/7780 |
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