Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells
Eggshell membrane (ESM) hydrolysate was prepared by Alcalase and Protease S (AL-PS) sequentially and was ultrafiltered as fractions AL-PS-I (<5 kDa), AL-PS-II (5-10 kDa), and AL-PS-III (>10 kDa). The antioxidant activity of ESM hydrolysate was evaluated using chemical assays and its protective...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-09-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464614001753 |
| _version_ | 1818417917636640768 |
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| author | Yaning Shi Jennifer Kovacs-Nolan Bo Jiang Rong Tsao Yoshinori Mine |
| author_facet | Yaning Shi Jennifer Kovacs-Nolan Bo Jiang Rong Tsao Yoshinori Mine |
| author_sort | Yaning Shi |
| collection | DOAJ |
| description | Eggshell membrane (ESM) hydrolysate was prepared by Alcalase and Protease S (AL-PS) sequentially and was ultrafiltered as fractions AL-PS-I (<5 kDa), AL-PS-II (5-10 kDa), and AL-PS-III (>10 kDa). The antioxidant activity of ESM hydrolysate was evaluated using chemical assays and its protective ability against oxidative stress was investigated using human intestinal epithelial Caco-2 cell-based assay. AL-PS-I possessed the highest scavenging activity against DPPH and hydroxyl radicals. AL-PS-III demonstrated the best Fe2+ chelating activity. AL-PS and AL-PS-I had higher cellular antioxidant activity to inhibit the formation of DCF. The secretion of IL-8 was inhibited significantly (P < 0.05) at 2 h post pretreatment with AL-PS, AL-PS-I and AL-PS-II (0.1 and 0.5 mg/mL) and at 6 h post stimulation with 1 mM H2O2. In addition, AL-PS, AL-PS-I and AL-PS-II (0.5 mg/mL) increased glutathione (GSH) level significantly (P < 0.05) in H2O2-treated Caco-2 cells. |
| first_indexed | 2024-12-14T12:14:24Z |
| format | Article |
| id | doaj.art-19c8322f06804344abd7793cf3a29be4 |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-14T12:14:24Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-19c8322f06804344abd7793cf3a29be42022-12-21T23:01:40ZengElsevierJournal of Functional Foods1756-46462014-09-01103545Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cellsYaning Shi0Jennifer Kovacs-Nolan1Bo Jiang2Rong Tsao3Yoshinori Mine4State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaGuelph Food Research Centre, Agriculture and Agri-Food Canada, 93 Stone Road West, Guelph, Ontario, Canada N1G 5C9State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Department of Food Science, University of Guelph, 50 Stone Road East, Guelph, Ontario, Canada N1G 2W1; Corresponding author. Tel.: +1 519 824 4120, ext. 52901; fax: +1 519 824 6631.Eggshell membrane (ESM) hydrolysate was prepared by Alcalase and Protease S (AL-PS) sequentially and was ultrafiltered as fractions AL-PS-I (<5 kDa), AL-PS-II (5-10 kDa), and AL-PS-III (>10 kDa). The antioxidant activity of ESM hydrolysate was evaluated using chemical assays and its protective ability against oxidative stress was investigated using human intestinal epithelial Caco-2 cell-based assay. AL-PS-I possessed the highest scavenging activity against DPPH and hydroxyl radicals. AL-PS-III demonstrated the best Fe2+ chelating activity. AL-PS and AL-PS-I had higher cellular antioxidant activity to inhibit the formation of DCF. The secretion of IL-8 was inhibited significantly (P < 0.05) at 2 h post pretreatment with AL-PS, AL-PS-I and AL-PS-II (0.1 and 0.5 mg/mL) and at 6 h post stimulation with 1 mM H2O2. In addition, AL-PS, AL-PS-I and AL-PS-II (0.5 mg/mL) increased glutathione (GSH) level significantly (P < 0.05) in H2O2-treated Caco-2 cells.http://www.sciencedirect.com/science/article/pii/S1756464614001753Eggshell membraneEnzymatic hydrolysisRadical scavenging activityOxidative stressCaco-2 |
| spellingShingle | Yaning Shi Jennifer Kovacs-Nolan Bo Jiang Rong Tsao Yoshinori Mine Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells Journal of Functional Foods Eggshell membrane Enzymatic hydrolysis Radical scavenging activity Oxidative stress Caco-2 |
| title | Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells |
| title_full | Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells |
| title_fullStr | Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells |
| title_full_unstemmed | Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells |
| title_short | Antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial Caco-2 cells |
| title_sort | antioxidant activity of enzymatic hydrolysates from eggshell membrane proteins and its protective capacity in human intestinal epithelial caco 2 cells |
| topic | Eggshell membrane Enzymatic hydrolysis Radical scavenging activity Oxidative stress Caco-2 |
| url | http://www.sciencedirect.com/science/article/pii/S1756464614001753 |
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