A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies
Radio frequency (RF) biosensors are an expanding field of interest because of the ability to design noninvasive, label-free, low-production-cost sensing devices. Previous works identified the need for smaller experimental devices, requiring nanoliter to milliliter sampling volumes and increased capa...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/23/11/5193 |
| _version_ | 1797596744557527040 |
|---|---|
| author | Mary Godfrey Daniel Ewert Ryan Striker Benjamin Braaten |
| author_facet | Mary Godfrey Daniel Ewert Ryan Striker Benjamin Braaten |
| author_sort | Mary Godfrey |
| collection | DOAJ |
| description | Radio frequency (RF) biosensors are an expanding field of interest because of the ability to design noninvasive, label-free, low-production-cost sensing devices. Previous works identified the need for smaller experimental devices, requiring nanoliter to milliliter sampling volumes and increased capability of repeatable and sensitive measurement capability. The following work aims to verify a millimeter-sized, microstrip transmission line biosensor design with a microliter well operating on a broadband radio frequency range of 1.0–17.0 GHz. Three successive experiments were performed to provide evidence for (1) repeatability of measurements after loading/unloading the well, (2) sensitivity of measurement sets, and (3) methodology verification. Materials under test (MUTs) loaded into the well included deionized water, Tris-EDTA buffer, and lambda DNA. S-parameters were measured to determine interaction levels between the radio frequencies and MUTs during the broadband sweep. MUTs increasing in concentration were repeatably detected and demonstrated high measurement sensitivity, with the highest error value observed being 0.36%. Comparing Tris-EDTA buffer versus lambda DNA suspended in Tris-EDTA buffer suggests that introducing lambda DNA into the Tris-EDTA buffer repeatably alters S-parameters. The innovative aspect of this biosensor is that it can measure interactions of electromagnetic energy and MUTs in microliter quantities with high repeatability and sensitivity. |
| first_indexed | 2024-03-11T02:56:22Z |
| format | Article |
| id | doaj.art-19ca45611bee492981d5eb53d132818d |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-11T02:56:22Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-19ca45611bee492981d5eb53d132818d2023-11-18T08:33:49ZengMDPI AGSensors1424-82202023-05-012311519310.3390/s23115193A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio FrequenciesMary Godfrey0Daniel Ewert1Ryan Striker2Benjamin Braaten3College of Engineering, North Dakota State University, Fargo, ND 58102, USACollege of Engineering and Mines, University of North Dakota, Grand Forks, ND 58202, USACollege of Engineering and Mines, University of North Dakota, Grand Forks, ND 58202, USACollege of Engineering, North Dakota State University, Fargo, ND 58102, USARadio frequency (RF) biosensors are an expanding field of interest because of the ability to design noninvasive, label-free, low-production-cost sensing devices. Previous works identified the need for smaller experimental devices, requiring nanoliter to milliliter sampling volumes and increased capability of repeatable and sensitive measurement capability. The following work aims to verify a millimeter-sized, microstrip transmission line biosensor design with a microliter well operating on a broadband radio frequency range of 1.0–17.0 GHz. Three successive experiments were performed to provide evidence for (1) repeatability of measurements after loading/unloading the well, (2) sensitivity of measurement sets, and (3) methodology verification. Materials under test (MUTs) loaded into the well included deionized water, Tris-EDTA buffer, and lambda DNA. S-parameters were measured to determine interaction levels between the radio frequencies and MUTs during the broadband sweep. MUTs increasing in concentration were repeatably detected and demonstrated high measurement sensitivity, with the highest error value observed being 0.36%. Comparing Tris-EDTA buffer versus lambda DNA suspended in Tris-EDTA buffer suggests that introducing lambda DNA into the Tris-EDTA buffer repeatably alters S-parameters. The innovative aspect of this biosensor is that it can measure interactions of electromagnetic energy and MUTs in microliter quantities with high repeatability and sensitivity.https://www.mdpi.com/1424-8220/23/11/5193RF biosensormicrostrip transmission line sensormicroliter sensorbiosensing |
| spellingShingle | Mary Godfrey Daniel Ewert Ryan Striker Benjamin Braaten A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies Sensors RF biosensor microstrip transmission line sensor microliter sensor biosensing |
| title | A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies |
| title_full | A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies |
| title_fullStr | A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies |
| title_full_unstemmed | A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies |
| title_short | A Microstrip Transmission Line Biosensor to Measure the Interaction between Microliter Aqueous Solutions and 1.0–17.0 GHz Radio Frequencies |
| title_sort | microstrip transmission line biosensor to measure the interaction between microliter aqueous solutions and 1 0 17 0 ghz radio frequencies |
| topic | RF biosensor microstrip transmission line sensor microliter sensor biosensing |
| url | https://www.mdpi.com/1424-8220/23/11/5193 |
| work_keys_str_mv | AT marygodfrey amicrostriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT danielewert amicrostriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT ryanstriker amicrostriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT benjaminbraaten amicrostriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT marygodfrey microstriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT danielewert microstriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT ryanstriker microstriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies AT benjaminbraaten microstriptransmissionlinebiosensortomeasuretheinteractionbetweenmicroliteraqueoussolutionsand10170ghzradiofrequencies |