Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays
The Caco-2 cell line derived from human colon carcinoma is commonly used to assess the permeability of compounds in in vitro conditions. Due to the significant increase in permeability studies using the Caco-2 cell line in recent years, the need to standardize this biological model seems necessary....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/11/2523 |
| _version_ | 1797458019676585984 |
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| author | Marta Kus Izabela Ibragimow Hanna Piotrowska-Kempisty |
| author_facet | Marta Kus Izabela Ibragimow Hanna Piotrowska-Kempisty |
| author_sort | Marta Kus |
| collection | DOAJ |
| description | The Caco-2 cell line derived from human colon carcinoma is commonly used to assess the permeability of compounds in in vitro conditions. Due to the significant increase in permeability studies using the Caco-2 cell line in recent years, the need to standardize this biological model seems necessary. The pharmaceutical requirements define only the acceptance criteria for the validation of the Caco-2 cell line and do not specify the protocol for its implementation. Therefore, the aim of this study is to review the conditions for permeability studies across the Caco-2 monolayer reported in the available literature concerning validation guidelines. We summarized the main aspects affecting the validation process of the Caco-2 cell line, including the culture conditions, cytotoxicity, cell differentiation process, and monolayer transport conditions, and the main conclusions may be useful in developing individual methods for preparing the cell line for validation purposes and further permeability research. |
| first_indexed | 2024-03-09T16:32:02Z |
| format | Article |
| id | doaj.art-19cc1185abf14af9ae3ed530048d6a4f |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T16:32:02Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-19cc1185abf14af9ae3ed530048d6a4f2023-11-24T15:00:42ZengMDPI AGPharmaceutics1999-49232023-10-011511252310.3390/pharmaceutics15112523Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability AssaysMarta Kus0Izabela Ibragimow1Hanna Piotrowska-Kempisty2Department of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, PolandResearch and Development Department of Ethifarm, Ethifarm Manufacturing Plant, 9 Stefana Zeromskiego St., 60-544 Poznan, PolandDepartment of Toxicology, Poznan University of Medical Sciences, 30 Dojazd St., 60-631 Poznan, PolandThe Caco-2 cell line derived from human colon carcinoma is commonly used to assess the permeability of compounds in in vitro conditions. Due to the significant increase in permeability studies using the Caco-2 cell line in recent years, the need to standardize this biological model seems necessary. The pharmaceutical requirements define only the acceptance criteria for the validation of the Caco-2 cell line and do not specify the protocol for its implementation. Therefore, the aim of this study is to review the conditions for permeability studies across the Caco-2 monolayer reported in the available literature concerning validation guidelines. We summarized the main aspects affecting the validation process of the Caco-2 cell line, including the culture conditions, cytotoxicity, cell differentiation process, and monolayer transport conditions, and the main conclusions may be useful in developing individual methods for preparing the cell line for validation purposes and further permeability research.https://www.mdpi.com/1999-4923/15/11/2523Caco-2 cell linevalidationdrug permeabilitypharmaceutical requirementsbiowaiver |
| spellingShingle | Marta Kus Izabela Ibragimow Hanna Piotrowska-Kempisty Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays Pharmaceutics Caco-2 cell line validation drug permeability pharmaceutical requirements biowaiver |
| title | Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays |
| title_full | Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays |
| title_fullStr | Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays |
| title_full_unstemmed | Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays |
| title_short | Caco-2 Cell Line Standardization with Pharmaceutical Requirements and In Vitro Model Suitability for Permeability Assays |
| title_sort | caco 2 cell line standardization with pharmaceutical requirements and in vitro model suitability for permeability assays |
| topic | Caco-2 cell line validation drug permeability pharmaceutical requirements biowaiver |
| url | https://www.mdpi.com/1999-4923/15/11/2523 |
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