Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress
The aim of the current study was to isolate endophytes from the roots of <i>Rhazya stricta</i> and assess their potential to improve the growth of drought-stressed sunflower seedlings. The potential role of the isolated endophytic fungus was initially screened by using two rice varieties...
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MDPI AG
2023-03-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/3/873 |
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| author | Nighat Seema Muhammad Hamayun Anwar Hussain Mohib Shah Muhammad Irshad Muhammad Qadir Amjad Iqbal Abdulwahed Fahad Alrefaei Sajid Ali |
| author_facet | Nighat Seema Muhammad Hamayun Anwar Hussain Mohib Shah Muhammad Irshad Muhammad Qadir Amjad Iqbal Abdulwahed Fahad Alrefaei Sajid Ali |
| author_sort | Nighat Seema |
| collection | DOAJ |
| description | The aim of the current study was to isolate endophytes from the roots of <i>Rhazya stricta</i> and assess their potential to improve the growth of drought-stressed sunflower seedlings. The potential role of the isolated endophytic fungus was initially screened by using two rice varieties (mutant rice cultivar <i>Waito-C</i>: Gibberellins deficient; and <i>Dongji byeo</i>: Gibberellins-producing normal cultivar). A significant (<i>p</i> < 0.05) increase in various growth attributes of both rice varieties associated with one of the isolates i.e., N4 was noticed. Furthermore, the N4 isolate was tested for its role in improving the agronomic attributes of sunflowers under drought stress. The symbiotic association significantly (<i>p</i> < 0.05) improved the host growth and protection from PEG-induced drought stress. The drought mitigation in N4-associated sunflower seedlings can be linked with the regulation of phytohormones, stress-related metabolites, low-molecular-weight proteins and sugars, and scavenging of reactive oxygen species (ROS). Microscopic analysis revealed that the potent endophytic fungal strain consisted of thin and hyaline hyphae, forming dense olive-green mycelia (4–5 cm in dm) with black flask-shaped fruiting bodies. Based on the ITS sequence homology and phylogeny, the strain was identified as <i>Fusarium proliferatum</i> (MG251448). The results of this study concluded that this phytohormone-secreting endophyte can improve crop productivity in dry areas where drought stress is the main challenge faced by crops. |
| first_indexed | 2024-03-11T07:02:12Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T07:02:12Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-19cc2be8b8e8457699e002be3f60849b2023-11-17T09:07:28ZengMDPI AGAgronomy2073-43952023-03-0113387310.3390/agronomy13030873Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought StressNighat Seema0Muhammad Hamayun1Anwar Hussain2Mohib Shah3Muhammad Irshad4Muhammad Qadir5Amjad Iqbal6Abdulwahed Fahad Alrefaei7Sajid Ali8Department of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Botany, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Food Science and Technology, Abdul Wali Khan University, Mardan 23200, PakistanDepartment of Zoology, College of Science, King Saud University, Riyadh 12372-2915, Saudi ArabiaDepartment of Horticulture and Life Science, Yeungnam University, Daegu 38541, Republic of KoreaThe aim of the current study was to isolate endophytes from the roots of <i>Rhazya stricta</i> and assess their potential to improve the growth of drought-stressed sunflower seedlings. The potential role of the isolated endophytic fungus was initially screened by using two rice varieties (mutant rice cultivar <i>Waito-C</i>: Gibberellins deficient; and <i>Dongji byeo</i>: Gibberellins-producing normal cultivar). A significant (<i>p</i> < 0.05) increase in various growth attributes of both rice varieties associated with one of the isolates i.e., N4 was noticed. Furthermore, the N4 isolate was tested for its role in improving the agronomic attributes of sunflowers under drought stress. The symbiotic association significantly (<i>p</i> < 0.05) improved the host growth and protection from PEG-induced drought stress. The drought mitigation in N4-associated sunflower seedlings can be linked with the regulation of phytohormones, stress-related metabolites, low-molecular-weight proteins and sugars, and scavenging of reactive oxygen species (ROS). Microscopic analysis revealed that the potent endophytic fungal strain consisted of thin and hyaline hyphae, forming dense olive-green mycelia (4–5 cm in dm) with black flask-shaped fruiting bodies. Based on the ITS sequence homology and phylogeny, the strain was identified as <i>Fusarium proliferatum</i> (MG251448). The results of this study concluded that this phytohormone-secreting endophyte can improve crop productivity in dry areas where drought stress is the main challenge faced by crops.https://www.mdpi.com/2073-4395/13/3/873drought<i>Fusarium proliferatum</i>sunflower plantsphytohormonesbiostimulant |
| spellingShingle | Nighat Seema Muhammad Hamayun Anwar Hussain Mohib Shah Muhammad Irshad Muhammad Qadir Amjad Iqbal Abdulwahed Fahad Alrefaei Sajid Ali Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress Agronomy drought <i>Fusarium proliferatum</i> sunflower plants phytohormones biostimulant |
| title | Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress |
| title_full | Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress |
| title_fullStr | Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress |
| title_full_unstemmed | Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress |
| title_short | Endophytic <i>Fusarium proliferatum</i> Reprogrammed Phytohormone Production and Antioxidant System of <i>Oryza sativa</i> under Drought Stress |
| title_sort | endophytic i fusarium proliferatum i reprogrammed phytohormone production and antioxidant system of i oryza sativa i under drought stress |
| topic | drought <i>Fusarium proliferatum</i> sunflower plants phytohormones biostimulant |
| url | https://www.mdpi.com/2073-4395/13/3/873 |
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