An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i>
A large number of RNA viruses have been discovered in most insect orders using high-throughput sequencing (HTS) and advanced bioinformatics methods. In this study, an RNA virome of the grasshopper was systematically identified in <i>Atractomorpha sinensis</i> (Orthoptera: Pyrgomorphidae)...
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MDPI AG
2022-12-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/1/9 |
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| author | Yu-Juan He Zhuang-Xin Ye Chuan-Xi Zhang Jun-Min Li Jian-Ping Chen Gang Lu |
| author_facet | Yu-Juan He Zhuang-Xin Ye Chuan-Xi Zhang Jun-Min Li Jian-Ping Chen Gang Lu |
| author_sort | Yu-Juan He |
| collection | DOAJ |
| description | A large number of RNA viruses have been discovered in most insect orders using high-throughput sequencing (HTS) and advanced bioinformatics methods. In this study, an RNA virome of the grasshopper was systematically identified in <i>Atractomorpha sinensis</i> (Orthoptera: Pyrgomorphidae), an important agricultural pest known as the pink-winged grasshopper. These insect viruses were classified as the nege-like virus, iflavirus, ollusvirus, and chu-like virus using HTS and phylogenetic analyses. Meanwhile, the full sequences of four novel RNA viruses were obtained with RACE and named Atractomorpha sinensis nege-like virus 1 (ASNV1), Atractomorpha sinensis iflavirus 1 (ASIV1), Atractomorpha sinensis ollusvirus 1 (ASOV1), and Atractomorpha sinensis chu-like virus 1 (ASCV1), respectively. Moreover, the analysis of virus-derived small interfering RNAs showed that most of the RNA viruses were targeted by the host antiviral RNA interference pathway. Moreover, our results provide a comprehensive analysis on the RNA virome of <i>A. sinensis</i>. |
| first_indexed | 2024-03-09T12:14:32Z |
| format | Article |
| id | doaj.art-19cf50ba83f045a080316c8145cc080e |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-09T12:14:32Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-19cf50ba83f045a080316c8145cc080e2023-11-30T22:47:40ZengMDPI AGInsects2075-44502022-12-01141910.3390/insects14010009An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i>Yu-Juan He0Zhuang-Xin Ye1Chuan-Xi Zhang2Jun-Min Li3Jian-Ping Chen4Gang Lu5State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of MARA and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, ChinaA large number of RNA viruses have been discovered in most insect orders using high-throughput sequencing (HTS) and advanced bioinformatics methods. In this study, an RNA virome of the grasshopper was systematically identified in <i>Atractomorpha sinensis</i> (Orthoptera: Pyrgomorphidae), an important agricultural pest known as the pink-winged grasshopper. These insect viruses were classified as the nege-like virus, iflavirus, ollusvirus, and chu-like virus using HTS and phylogenetic analyses. Meanwhile, the full sequences of four novel RNA viruses were obtained with RACE and named Atractomorpha sinensis nege-like virus 1 (ASNV1), Atractomorpha sinensis iflavirus 1 (ASIV1), Atractomorpha sinensis ollusvirus 1 (ASOV1), and Atractomorpha sinensis chu-like virus 1 (ASCV1), respectively. Moreover, the analysis of virus-derived small interfering RNAs showed that most of the RNA viruses were targeted by the host antiviral RNA interference pathway. Moreover, our results provide a comprehensive analysis on the RNA virome of <i>A. sinensis</i>.https://www.mdpi.com/2075-4450/14/1/9<i>Atractomorpha sinensis</i>grasshopperRNA viromehigh-throughput sequencingRNA interference pathway |
| spellingShingle | Yu-Juan He Zhuang-Xin Ye Chuan-Xi Zhang Jun-Min Li Jian-Ping Chen Gang Lu An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> Insects <i>Atractomorpha sinensis</i> grasshopper RNA virome high-throughput sequencing RNA interference pathway |
| title | An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> |
| title_full | An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> |
| title_fullStr | An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> |
| title_full_unstemmed | An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> |
| title_short | An RNA Virome Analysis of the Pink-Winged Grasshopper <i>Atractomorpha sinensis</i> |
| title_sort | rna virome analysis of the pink winged grasshopper i atractomorpha sinensis i |
| topic | <i>Atractomorpha sinensis</i> grasshopper RNA virome high-throughput sequencing RNA interference pathway |
| url | https://www.mdpi.com/2075-4450/14/1/9 |
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