Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil
We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains w...
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2011-05-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003&lng=en&tlng=en |
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| author | Silvana Spíndola de Miranda Wânia da Silva Carvalho Philip Noel Suffys Afrânio Lineu Kritski Martha Oliveira Noemi Zarate Thierry Zozio Nalin Rastogi Brigitte Gicquel |
| author_facet | Silvana Spíndola de Miranda Wânia da Silva Carvalho Philip Noel Suffys Afrânio Lineu Kritski Martha Oliveira Noemi Zarate Thierry Zozio Nalin Rastogi Brigitte Gicquel |
| author_sort | Silvana Spíndola de Miranda |
| collection | DOAJ |
| description | We performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region. |
| first_indexed | 2024-03-12T18:06:06Z |
| format | Article |
| id | doaj.art-19d1d27797864cb6936a826008109908 |
| institution | Directory Open Access Journal |
| issn | 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:06:06Z |
| publishDate | 2011-05-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-19d1d27797864cb6936a8260081099082023-08-02T09:28:19ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz1678-80602011-05-01106326727310.1590/S0074-02762011000300003S0074-02762011000300003Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, BrazilSilvana Spíndola de Miranda0Wânia da Silva Carvalho1Philip Noel Suffys2Afrânio Lineu Kritski3Martha Oliveira4Noemi Zarate5Thierry Zozio6Nalin Rastogi7Brigitte GicquelUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisUniversidade Federal de Minas GeraisWe performed spoligotyping on 114 strains of the Mycobacterium tuberculosis (Mtb) complex that had been isolated from patients in Minas Gerais Health Units during 2004. A total of 82/114 (72%) clinical isolates were clustered and 32/114 (28%) were unique. Seven shared types containing nine strains were newly created. A total of nine patterns corresponded to unreported orphan strains, as evaluated against all of the strains recorded in the SITVIT2 proprietary database in the Institut Pasteur de la Guadeloupe. The major clades were composed of isolates that belong to the following genotypes: Latin-America and Mediterranean (63/114, 55.3%) (the ill-defined T superfamily) (12/114, 10.5%), Haarlem (8/114, 7%), X clade (6/114, 5.3%), S clade (3/114, 2.6%) and the East-African Indian and Manu types, each with 1/114 (0.9%) isolates. A considerable number of strains (n = 20, 17.5%) showed patterns that did not fall within any of the previously described major clades. We conclude the bulk of tuberculosis (TB) (92/114, 80.7%) in our location is recent evolutionary strains that belong to the principal genetic groups 2/3. Further studies on epidemiology of TB are required to understand Mtb biodiversity and TB transmission in this region.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003&lng=en&tlng=enMycobacterium tuberculosisspoligotypingBrazilMinas Gerais |
| spellingShingle | Silvana Spíndola de Miranda Wânia da Silva Carvalho Philip Noel Suffys Afrânio Lineu Kritski Martha Oliveira Noemi Zarate Thierry Zozio Nalin Rastogi Brigitte Gicquel Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil Memorias do Instituto Oswaldo Cruz Mycobacterium tuberculosis spoligotyping Brazil Minas Gerais |
| title | Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil |
| title_full | Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil |
| title_fullStr | Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil |
| title_full_unstemmed | Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil |
| title_short | Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil |
| title_sort | spoligotyping of clinical mycobacterium tuberculosis isolates from the state of minas gerais brazil |
| topic | Mycobacterium tuberculosis spoligotyping Brazil Minas Gerais |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000300003&lng=en&tlng=en |
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