Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice
Ceramide sphingolipids are major components of membranes. C2 and C6 ceramides induce programmed cell death (PCD) in animals and plants, and we previously showed that C2 and C6 ceramides induce PCD in rice (Oryza sativa) protoplasts. However, the mechanistic link between sphingolipids and PCD in rice...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fpls.2020.00145/full |
| _version_ | 1818326985447833600 |
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| author | Quan-Fang Zhang Jian Li Fang-Cheng Bi Zhe Liu Zhen-Yi Chang Ling-Yan Wang Li-Qun Huang Nan Yao |
| author_facet | Quan-Fang Zhang Jian Li Fang-Cheng Bi Zhe Liu Zhen-Yi Chang Ling-Yan Wang Li-Qun Huang Nan Yao |
| author_sort | Quan-Fang Zhang |
| collection | DOAJ |
| description | Ceramide sphingolipids are major components of membranes. C2 and C6 ceramides induce programmed cell death (PCD) in animals and plants, and we previously showed that C2 and C6 ceramides induce PCD in rice (Oryza sativa) protoplasts. However, the mechanistic link between sphingolipids and PCD in rice remains unclear. Here, we observed that calcium levels increased rapidly after ceramide treatment. Moreover, the calcium channel inhibitor LaCl3 and the intracellular calcium chelator acetoxymethyl-1, 2-bis (2-aminophenoxy) ethic acid (BAPTA-AM) inhibited this calcium increase and prevented ceramide-induced PCD. Moreover, caspase-3-like protease activity increased significantly in C6 ceramide-treated protoplasts, and a caspase-specific inhibitor prevented C6 ceramide-induced cell death. We also detected the other typical PCD events including ATP loss. DIDS (4, 49-diisothiocyanatostilbene- 2, 29-disulfonic acid), an inhibitor of voltage-dependent anion channels (VDACs), decreased C6 ceramide-induced cell death. Together, this evidence suggests that mitochondria played an important role in C6 ceramide-induced PCD. |
| first_indexed | 2024-12-13T12:09:04Z |
| format | Article |
| id | doaj.art-19d3dab009544b4c952e7ab737dae705 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-13T12:09:04Z |
| publishDate | 2020-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-19d3dab009544b4c952e7ab737dae7052022-12-21T23:46:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2020-02-011110.3389/fpls.2020.00145477141Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in RiceQuan-Fang ZhangJian LiFang-Cheng BiZhe LiuZhen-Yi ChangLing-Yan WangLi-Qun HuangNan YaoCeramide sphingolipids are major components of membranes. C2 and C6 ceramides induce programmed cell death (PCD) in animals and plants, and we previously showed that C2 and C6 ceramides induce PCD in rice (Oryza sativa) protoplasts. However, the mechanistic link between sphingolipids and PCD in rice remains unclear. Here, we observed that calcium levels increased rapidly after ceramide treatment. Moreover, the calcium channel inhibitor LaCl3 and the intracellular calcium chelator acetoxymethyl-1, 2-bis (2-aminophenoxy) ethic acid (BAPTA-AM) inhibited this calcium increase and prevented ceramide-induced PCD. Moreover, caspase-3-like protease activity increased significantly in C6 ceramide-treated protoplasts, and a caspase-specific inhibitor prevented C6 ceramide-induced cell death. We also detected the other typical PCD events including ATP loss. DIDS (4, 49-diisothiocyanatostilbene- 2, 29-disulfonic acid), an inhibitor of voltage-dependent anion channels (VDACs), decreased C6 ceramide-induced cell death. Together, this evidence suggests that mitochondria played an important role in C6 ceramide-induced PCD.https://www.frontiersin.org/article/10.3389/fpls.2020.00145/fullcalciumcaspaseceramideprogrammed cell deathrice |
| spellingShingle | Quan-Fang Zhang Jian Li Fang-Cheng Bi Zhe Liu Zhen-Yi Chang Ling-Yan Wang Li-Qun Huang Nan Yao Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice Frontiers in Plant Science calcium caspase ceramide programmed cell death rice |
| title | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
| title_full | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
| title_fullStr | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
| title_full_unstemmed | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
| title_short | Ceramide-Induced Cell Death Depends on Calcium and Caspase-Like Activity in Rice |
| title_sort | ceramide induced cell death depends on calcium and caspase like activity in rice |
| topic | calcium caspase ceramide programmed cell death rice |
| url | https://www.frontiersin.org/article/10.3389/fpls.2020.00145/full |
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