Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i>
The lungworm <i>Dictyocaulus viviparus</i> is one of the most economically important bovine parasites in temperate climate regions. Following infection, <i>D. viviparus</i> induces a temporary protective immunity, and a vaccine based on attenuated, infective larvae is commerc...
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MDPI AG
2022-01-01
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| author | Andrea Springer Christian von Holtum Georg von Samson-Himmelstjerna Christina Strube |
| author_facet | Andrea Springer Christian von Holtum Georg von Samson-Himmelstjerna Christina Strube |
| author_sort | Andrea Springer |
| collection | DOAJ |
| description | The lungworm <i>Dictyocaulus viviparus</i> is one of the most economically important bovine parasites in temperate climate regions. Following infection, <i>D. viviparus</i> induces a temporary protective immunity, and a vaccine based on attenuated, infective larvae is commercially available. However, due to several disadvantages of the live vaccine, the development of a recombinant subunit vaccine is highly desirable. Therefore, the major sperm protein (MSP), which is essential for the parasite’s reproduction, was tested as a recombinantly <i>Escherichia coli</i>-expressed glutathione-S-transferase (GST)-fused vaccine antigen in immunization trials with two different adjuvants, Quil A and Al(OH)<sub>3</sub>. Calves (N = 4 per group) were immunized on study day (SD) 0, 21 and 42 and given a challenge infection on SD 63–65. The two control groups received only the respective adjuvant. Based on geometric means (GM), a 53.64% reduction in larvae per female worm was observed in the rMSP Quil A group vs. its control group (arithmetic means (AM): 54.43%), but this difference was not statistically significant. In the rMSP Al(OH)<sub>3</sub> group, the mean number of larvae per female worm was even higher than in the respective control group (GM: 9.24%, AM: 14.14%). Furthermore, male and female worm burdens and the absolute number of larvae did not differ significantly, while the Al(OH)<sub>3</sub> control group harbored significantly longer worms than the vaccinated group. Vaccinated animals showed a rise in rMSP-specific antibodies, particularly IgG and its subclass IgG1, and the native protein was detected by immunoblots. Although rMSP alone did not lead to significantly reduced worm fecundity, it might still prove useful as part of a multi-component vaccine. |
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| last_indexed | 2024-03-10T00:45:49Z |
| publishDate | 2022-01-01 |
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| series | Pathogens |
| spelling | doaj.art-19d45b6d54614f179dedf95fba2309502023-11-23T14:59:41ZengMDPI AGPathogens2076-08172022-01-011115510.3390/pathogens11010055Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i>Andrea Springer0Christian von Holtum1Georg von Samson-Himmelstjerna2Christina Strube3Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyThe lungworm <i>Dictyocaulus viviparus</i> is one of the most economically important bovine parasites in temperate climate regions. Following infection, <i>D. viviparus</i> induces a temporary protective immunity, and a vaccine based on attenuated, infective larvae is commercially available. However, due to several disadvantages of the live vaccine, the development of a recombinant subunit vaccine is highly desirable. Therefore, the major sperm protein (MSP), which is essential for the parasite’s reproduction, was tested as a recombinantly <i>Escherichia coli</i>-expressed glutathione-S-transferase (GST)-fused vaccine antigen in immunization trials with two different adjuvants, Quil A and Al(OH)<sub>3</sub>. Calves (N = 4 per group) were immunized on study day (SD) 0, 21 and 42 and given a challenge infection on SD 63–65. The two control groups received only the respective adjuvant. Based on geometric means (GM), a 53.64% reduction in larvae per female worm was observed in the rMSP Quil A group vs. its control group (arithmetic means (AM): 54.43%), but this difference was not statistically significant. In the rMSP Al(OH)<sub>3</sub> group, the mean number of larvae per female worm was even higher than in the respective control group (GM: 9.24%, AM: 14.14%). Furthermore, male and female worm burdens and the absolute number of larvae did not differ significantly, while the Al(OH)<sub>3</sub> control group harbored significantly longer worms than the vaccinated group. Vaccinated animals showed a rise in rMSP-specific antibodies, particularly IgG and its subclass IgG1, and the native protein was detected by immunoblots. Although rMSP alone did not lead to significantly reduced worm fecundity, it might still prove useful as part of a multi-component vaccine.https://www.mdpi.com/2076-0817/11/1/55major sperm protein<i>Dictyocaulus viviparus</i>recombinant vaccinevaccinationimmunization |
| spellingShingle | Andrea Springer Christian von Holtum Georg von Samson-Himmelstjerna Christina Strube Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> Pathogens major sperm protein <i>Dictyocaulus viviparus</i> recombinant vaccine vaccination immunization |
| title | Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> |
| title_full | Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> |
| title_fullStr | Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> |
| title_full_unstemmed | Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> |
| title_short | Immunization Trials with Recombinant Major Sperm Protein of the Bovine Lungworm <i>Dictyocaulus viviparus</i> |
| title_sort | immunization trials with recombinant major sperm protein of the bovine lungworm i dictyocaulus viviparus i |
| topic | major sperm protein <i>Dictyocaulus viviparus</i> recombinant vaccine vaccination immunization |
| url | https://www.mdpi.com/2076-0817/11/1/55 |
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