Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b
The neuropeptide Y (NPY) system influences numerous physiological functions including feeding behavior, endocrine regulation, and cardiovascular regulation. In jawed vertebrates it consists of 3-4 peptides and 4-7 receptors. Teleost fishes have unique duplicates of NPY and PYY as well as the Y8 rece...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2013-03-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00029/full |
| _version_ | 1818108696965677056 |
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| author | Görel eSundström Tomas A Larsson Bo eXu Johan eHeldin Dan eLarhammar |
| author_facet | Görel eSundström Tomas A Larsson Bo eXu Johan eHeldin Dan eLarhammar |
| author_sort | Görel eSundström |
| collection | DOAJ |
| description | The neuropeptide Y (NPY) system influences numerous physiological functions including feeding behavior, endocrine regulation, and cardiovascular regulation. In jawed vertebrates it consists of 3-4 peptides and 4-7 receptors. Teleost fishes have unique duplicates of NPY and PYY as well as the Y8 receptor. In the zebrafish, the NPY system consists of the peptides NPYa, PYYa, and PYYb (NPYb appears to have been lost) and at least seven NPY receptors: Y1, Y2, Y2-2, Y4, Y7, Y8a and Y8b. Previously PYYb binding has been reported for Y2 and Y2-2. To search for peptide-receptor preferences, we have investigated PYYb binding to four of the remaining receptors and compared with NPYa and PYYa. Taken together, the most striking observations are that PYYa displays reduced affinity for Y2 (3 nM) compared to the other peptides and receptors and that all three peptides have higher affinity for Y4 (0.028-0.034 nM) than for the other five receptors. The strongest peptide preference by any receptor selectivity is the one previously reported for PYYb by the Y2 receptor, as compared to NPY and PYYa. These affinity differences may be helpful to elucidate specific details of peptide-receptor interactions. Also, we have investigated the level of mRNA expression in different organs using qPCR. All peptides and receptors have higher expression in heart, kidney and brain. These quantitative aspects on receptor affinities and mRNA distribution help provide a more complete picture of the NPY system. |
| first_indexed | 2024-12-11T02:19:28Z |
| format | Article |
| id | doaj.art-19d5c46b05ee47cabbad22105c22f44a |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-12-11T02:19:28Z |
| publishDate | 2013-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-19d5c46b05ee47cabbad22105c22f44a2022-12-22T01:24:05ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2013-03-01710.3389/fnins.2013.0002941156Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8bGörel eSundström0Tomas A Larsson1Bo eXu2Johan eHeldin3Dan eLarhammar4Uppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityUppsala UniversityThe neuropeptide Y (NPY) system influences numerous physiological functions including feeding behavior, endocrine regulation, and cardiovascular regulation. In jawed vertebrates it consists of 3-4 peptides and 4-7 receptors. Teleost fishes have unique duplicates of NPY and PYY as well as the Y8 receptor. In the zebrafish, the NPY system consists of the peptides NPYa, PYYa, and PYYb (NPYb appears to have been lost) and at least seven NPY receptors: Y1, Y2, Y2-2, Y4, Y7, Y8a and Y8b. Previously PYYb binding has been reported for Y2 and Y2-2. To search for peptide-receptor preferences, we have investigated PYYb binding to four of the remaining receptors and compared with NPYa and PYYa. Taken together, the most striking observations are that PYYa displays reduced affinity for Y2 (3 nM) compared to the other peptides and receptors and that all three peptides have higher affinity for Y4 (0.028-0.034 nM) than for the other five receptors. The strongest peptide preference by any receptor selectivity is the one previously reported for PYYb by the Y2 receptor, as compared to NPY and PYYa. These affinity differences may be helpful to elucidate specific details of peptide-receptor interactions. Also, we have investigated the level of mRNA expression in different organs using qPCR. All peptides and receptors have higher expression in heart, kidney and brain. These quantitative aspects on receptor affinities and mRNA distribution help provide a more complete picture of the NPY system.http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00029/fullZebrafishevolutionNPYPYYG-protein-coupled receptor |
| spellingShingle | Görel eSundström Tomas A Larsson Bo eXu Johan eHeldin Dan eLarhammar Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b Frontiers in Neuroscience Zebrafish evolution NPY PYY G-protein-coupled receptor |
| title | Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b |
| title_full | Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b |
| title_fullStr | Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b |
| title_full_unstemmed | Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b |
| title_short | Interactions of zebrafish peptide YYb with the neuropeptide Y-family receptors Y4, Y7, Y8a and Y8b |
| title_sort | interactions of zebrafish peptide yyb with the neuropeptide y family receptors y4 y7 y8a and y8b |
| topic | Zebrafish evolution NPY PYY G-protein-coupled receptor |
| url | http://journal.frontiersin.org/Journal/10.3389/fnins.2013.00029/full |
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