Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition
Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, the single-cell or low-input proteomic research in this field is relatively underdeveloped because of the higher threshold of the starting material for mammalian embryo samp...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Pharmaceutical Analysis |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095177923000916 |
| _version_ | 1797733047260413952 |
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| author | Lei Gu Xumiao Li Wencheng Zhu Yi Shen Qinqin Wang Wenjun Liu Junfeng Zhang Huiping Zhang Jingquan Li Ziyi Li Zhen Liu Chen Li Hui Wang |
| author_facet | Lei Gu Xumiao Li Wencheng Zhu Yi Shen Qinqin Wang Wenjun Liu Junfeng Zhang Huiping Zhang Jingquan Li Ziyi Li Zhen Liu Chen Li Hui Wang |
| author_sort | Lei Gu |
| collection | DOAJ |
| description | Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, the single-cell or low-input proteomic research in this field is relatively underdeveloped because of the higher threshold of the starting material for mammalian embryo samples and the lack of hypersensitive proteome technology. In this study, a comprehensive solution of ultrasensitive proteome technology (CS-UPT) was developed for single-cell or low-input mouse oocyte/embryo samples. The deep coverage and high-throughput routes significantly reduced the starting material and were selected by investigators based on their demands. Using the deep coverage route, we provided the first large-scale snapshot of the very early stage of mouse maternal-to-zygotic transition, including almost 5,500 protein groups from 20 mouse oocytes or zygotes for each sample. Moreover, significant protein regulatory networks centered on transcription factors and kinases between the MII oocyte and 1-cell embryo provided rich insights into minor zygotic genome activation. |
| first_indexed | 2024-03-12T12:23:23Z |
| format | Article |
| id | doaj.art-19d66c4eb9084a8987015f6105be7494 |
| institution | Directory Open Access Journal |
| issn | 2095-1779 |
| language | English |
| last_indexed | 2024-03-12T12:23:23Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmaceutical Analysis |
| spelling | doaj.art-19d66c4eb9084a8987015f6105be74942023-08-30T05:50:23ZengElsevierJournal of Pharmaceutical Analysis2095-17792023-08-01138942954Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transitionLei Gu0Xumiao Li1Wencheng Zhu2Yi Shen3Qinqin Wang4Wenjun Liu5Junfeng Zhang6Huiping Zhang7Jingquan Li8Ziyi Li9Zhen Liu10Chen Li11Hui Wang12Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, ChinaShanghai Applied Protein Technology Co., Ltd., Shanghai, 201100, ChinaCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaInstitute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China; University of Chinese Academy of Sciences, Beijing, 101408, ChinaShanghai Applied Protein Technology Co., Ltd., Shanghai, 201100, ChinaShanghai Applied Protein Technology Co., Ltd., Shanghai, 201100, ChinaCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, ChinaShanghai Applied Protein Technology Co., Ltd., Shanghai, 201100, China; Corresponding author.Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China; Shanghai Center for Brain Science and Brain-Inspired Intelligence Technology, Shanghai, 200031, China; Corresponding author. Institute of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, CAS Key Laboratory of Primate Neurobiology, State Key Laboratory of Neuroscience, Chinese Academy of Sciences, Shanghai, 200031, China.Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; Corresponding author.Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China; Corresponding author.Single-cell or low-input multi-omics techniques have revolutionized the study of pre-implantation embryo development. However, the single-cell or low-input proteomic research in this field is relatively underdeveloped because of the higher threshold of the starting material for mammalian embryo samples and the lack of hypersensitive proteome technology. In this study, a comprehensive solution of ultrasensitive proteome technology (CS-UPT) was developed for single-cell or low-input mouse oocyte/embryo samples. The deep coverage and high-throughput routes significantly reduced the starting material and were selected by investigators based on their demands. Using the deep coverage route, we provided the first large-scale snapshot of the very early stage of mouse maternal-to-zygotic transition, including almost 5,500 protein groups from 20 mouse oocytes or zygotes for each sample. Moreover, significant protein regulatory networks centered on transcription factors and kinases between the MII oocyte and 1-cell embryo provided rich insights into minor zygotic genome activation.http://www.sciencedirect.com/science/article/pii/S2095177923000916Single-cell proteomicsLow-input proteomicsMaternal-to-zygotic transitionOocyteEmbryo |
| spellingShingle | Lei Gu Xumiao Li Wencheng Zhu Yi Shen Qinqin Wang Wenjun Liu Junfeng Zhang Huiping Zhang Jingquan Li Ziyi Li Zhen Liu Chen Li Hui Wang Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition Journal of Pharmaceutical Analysis Single-cell proteomics Low-input proteomics Maternal-to-zygotic transition Oocyte Embryo |
| title | Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition |
| title_full | Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition |
| title_fullStr | Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition |
| title_full_unstemmed | Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition |
| title_short | Ultrasensitive proteomics depicted an in-depth landscape for the very early stage of mouse maternal-to-zygotic transition |
| title_sort | ultrasensitive proteomics depicted an in depth landscape for the very early stage of mouse maternal to zygotic transition |
| topic | Single-cell proteomics Low-input proteomics Maternal-to-zygotic transition Oocyte Embryo |
| url | http://www.sciencedirect.com/science/article/pii/S2095177923000916 |
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