Covalent immobilization of lipase from Candida rugosa on Eupergit®
An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit® with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Faculty of Technology, Novi Sad
2005-01-01
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| Series: | Acta Periodica Technologica |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/1450-7188/2005/1450-71880536179B.pdf |
| _version_ | 1818567848004419584 |
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| author | Bezbradica Dejan I. Ćorović Jasmina J. Prodanović Radivoje M. Milosavić Nenad B. Knežević Zorica D. |
| author_facet | Bezbradica Dejan I. Ćorović Jasmina J. Prodanović Radivoje M. Milosavić Nenad B. Knežević Zorica D. |
| author_sort | Bezbradica Dejan I. |
| collection | DOAJ |
| description | An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit® with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of the enzyme functional groups that are probably important in the catalytic mechanism. The hydrolytic activities of the lipase immobilized on Eupergif1 by two conventional methods (via oxirane group and via glutaralde-hyde) and with periodate method were compared. Results of lipase assays suggest that periodate method is superior for lipase immobilization on Eupergit® among methods applied in this study with respect to both, yield of immobilization and hydrolytic activity of the immobilized enzyme. |
| first_indexed | 2024-12-14T06:28:36Z |
| format | Article |
| id | doaj.art-19d7a324ed7545d5b349e2d9afeb96be |
| institution | Directory Open Access Journal |
| issn | 1450-7188 |
| language | English |
| last_indexed | 2024-12-14T06:28:36Z |
| publishDate | 2005-01-01 |
| publisher | Faculty of Technology, Novi Sad |
| record_format | Article |
| series | Acta Periodica Technologica |
| spelling | doaj.art-19d7a324ed7545d5b349e2d9afeb96be2022-12-21T23:13:35ZengFaculty of Technology, Novi SadActa Periodica Technologica1450-71882005-01-0120053617918610.2298/APT0536179BCovalent immobilization of lipase from Candida rugosa on Eupergit®Bezbradica Dejan I.Ćorović Jasmina J.Prodanović Radivoje M.Milosavić Nenad B.Knežević Zorica D.An approach is presented for the stable covalent immobilization of Upase from Candida rugosa on Eupergit® with a high retention of hydrolytic activity. It comprises covalent bonding via lipase carbohydrate moiety previously modified by periodate oxidation, allowing a reduction in the involvement of the enzyme functional groups that are probably important in the catalytic mechanism. The hydrolytic activities of the lipase immobilized on Eupergif1 by two conventional methods (via oxirane group and via glutaralde-hyde) and with periodate method were compared. Results of lipase assays suggest that periodate method is superior for lipase immobilization on Eupergit® among methods applied in this study with respect to both, yield of immobilization and hydrolytic activity of the immobilized enzyme.http://www.doiserbia.nb.rs/img/doi/1450-7188/2005/1450-71880536179B.pdfEupergit®lipaseCandida rugosa'immobilization |
| spellingShingle | Bezbradica Dejan I. Ćorović Jasmina J. Prodanović Radivoje M. Milosavić Nenad B. Knežević Zorica D. Covalent immobilization of lipase from Candida rugosa on Eupergit® Acta Periodica Technologica Eupergit® lipase Candida rugosa' immobilization |
| title | Covalent immobilization of lipase from Candida rugosa on Eupergit® |
| title_full | Covalent immobilization of lipase from Candida rugosa on Eupergit® |
| title_fullStr | Covalent immobilization of lipase from Candida rugosa on Eupergit® |
| title_full_unstemmed | Covalent immobilization of lipase from Candida rugosa on Eupergit® |
| title_short | Covalent immobilization of lipase from Candida rugosa on Eupergit® |
| title_sort | covalent immobilization of lipase from candida rugosa on eupergit r |
| topic | Eupergit® lipase Candida rugosa' immobilization |
| url | http://www.doiserbia.nb.rs/img/doi/1450-7188/2005/1450-71880536179B.pdf |
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