The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses
The accumulation of the auxin precursor indole-3-acetamide (IAM) in the ami1 mutant has recently been reported to reduce plant growth and to trigger abiotic stress responses in Arabidopsis thaliana. The observed response includes the induction of abscisic acid (ABA) biosynthesis through the promotio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2022.928386/full |
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| author | Paloma Ortiz-García Marta-Marina Pérez-Alonso Marta-Marina Pérez-Alonso Adrián González Ortega-Villaizán Beatriz Sánchez-Parra Beatriz Sánchez-Parra Jutta Ludwig-Müller Mark D. Wilkinson Stephan Pollmann Stephan Pollmann |
| author_facet | Paloma Ortiz-García Marta-Marina Pérez-Alonso Marta-Marina Pérez-Alonso Adrián González Ortega-Villaizán Beatriz Sánchez-Parra Beatriz Sánchez-Parra Jutta Ludwig-Müller Mark D. Wilkinson Stephan Pollmann Stephan Pollmann |
| author_sort | Paloma Ortiz-García |
| collection | DOAJ |
| description | The accumulation of the auxin precursor indole-3-acetamide (IAM) in the ami1 mutant has recently been reported to reduce plant growth and to trigger abiotic stress responses in Arabidopsis thaliana. The observed response includes the induction of abscisic acid (ABA) biosynthesis through the promotion of NCED3 expression. The mechanism by which plant growth is limited, however, remained largely unclear. Here, we investigated the transcriptional responses evoked by the exogenous application of IAM using comprehensive RNA-sequencing (RNA-seq) and reverse genetics approaches. The RNA-seq results highlighted the induction of a small number of genes, including the R2R3 MYB transcription factor genes MYB74 and MYB102. The two MYB factors are known to respond to various stress cues and to ABA. Consistent with a role as negative plant growth regulator, conditional MYB74 overexpressor lines showed a considerable growth reduction. RNA-seq analysis of MYB74 mutants indicated an association of MYB74 with responses to osmotic stress, water deprivation, and seed development, which further linked MYB74 with the observed ami1 osmotic stress and seed phenotype. Collectively, our findings point toward a role for MYB74 in plant growth control and in responses to abiotic stress stimuli. |
| first_indexed | 2024-04-13T16:53:45Z |
| format | Article |
| id | doaj.art-19dcbefe04f04aa693fa25ea1685bcf3 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-13T16:53:45Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-19dcbefe04f04aa693fa25ea1685bcf32022-12-22T02:38:52ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2022-06-011310.3389/fpls.2022.928386928386The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress ResponsesPaloma Ortiz-García0Marta-Marina Pérez-Alonso1Marta-Marina Pérez-Alonso2Adrián González Ortega-Villaizán3Beatriz Sánchez-Parra4Beatriz Sánchez-Parra5Jutta Ludwig-Müller6Mark D. Wilkinson7Stephan Pollmann8Stephan Pollmann9Centro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainCentro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainUmeå Plant Science Center, Umeå University, Umeå, SwedenCentro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainCentro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainInstitute of Biology, University of Graz, Graz, AustriaInstitute of Botany, Technische Universität Dresden, Dresden, GermanyCentro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainCentro de Biotecnología y Genómica de Plantas,Universidad Politécnica de Madrid (UPM)–Instituto Nacional de Investigación y Tecnología Agraria y Alimentación (INIA /CSIC), Madrid, SpainDepartamento de Biotecnología-Biología Vegetal, Escuela Técnica Superior de Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid (UPM), Madrid, SpainThe accumulation of the auxin precursor indole-3-acetamide (IAM) in the ami1 mutant has recently been reported to reduce plant growth and to trigger abiotic stress responses in Arabidopsis thaliana. The observed response includes the induction of abscisic acid (ABA) biosynthesis through the promotion of NCED3 expression. The mechanism by which plant growth is limited, however, remained largely unclear. Here, we investigated the transcriptional responses evoked by the exogenous application of IAM using comprehensive RNA-sequencing (RNA-seq) and reverse genetics approaches. The RNA-seq results highlighted the induction of a small number of genes, including the R2R3 MYB transcription factor genes MYB74 and MYB102. The two MYB factors are known to respond to various stress cues and to ABA. Consistent with a role as negative plant growth regulator, conditional MYB74 overexpressor lines showed a considerable growth reduction. RNA-seq analysis of MYB74 mutants indicated an association of MYB74 with responses to osmotic stress, water deprivation, and seed development, which further linked MYB74 with the observed ami1 osmotic stress and seed phenotype. Collectively, our findings point toward a role for MYB74 in plant growth control and in responses to abiotic stress stimuli.https://www.frontiersin.org/articles/10.3389/fpls.2022.928386/fullauxinplant hormone crosstalkgrowth repressionplant stress responseArabidopsis thaliana |
| spellingShingle | Paloma Ortiz-García Marta-Marina Pérez-Alonso Marta-Marina Pérez-Alonso Adrián González Ortega-Villaizán Beatriz Sánchez-Parra Beatriz Sánchez-Parra Jutta Ludwig-Müller Mark D. Wilkinson Stephan Pollmann Stephan Pollmann The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses Frontiers in Plant Science auxin plant hormone crosstalk growth repression plant stress response Arabidopsis thaliana |
| title | The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses |
| title_full | The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses |
| title_fullStr | The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses |
| title_full_unstemmed | The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses |
| title_short | The Indole-3-Acetamide-Induced Arabidopsis Transcription Factor MYB74 Decreases Plant Growth and Contributes to the Control of Osmotic Stress Responses |
| title_sort | indole 3 acetamide induced arabidopsis transcription factor myb74 decreases plant growth and contributes to the control of osmotic stress responses |
| topic | auxin plant hormone crosstalk growth repression plant stress response Arabidopsis thaliana |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2022.928386/full |
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