Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer
Currently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepa...
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MDPI AG
2020-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/14/4828 |
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| author | Edyta Barnas Joanna Skret-Magierlo Andrzej Skret Ewa Kaznowska Joanna Depciuch Kamil Szmuc Kornelia Łach Izabela Krawczyk-Marć Jozef Cebulski |
| author_facet | Edyta Barnas Joanna Skret-Magierlo Andrzej Skret Ewa Kaznowska Joanna Depciuch Kamil Szmuc Kornelia Łach Izabela Krawczyk-Marć Jozef Cebulski |
| author_sort | Edyta Barnas |
| collection | DOAJ |
| description | Currently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepancies in reassessment. Therefore, there is a need to create methods of objective evaluation allowing the diagnosis of early changes. The study aimed to simultaneously asses Fourier Transform Infrared (FTIR) and Raman spectroscopy combined with multidimensional analysis to identify the tissues of endometrial cancer, atypical hyperplasia and the normal control group, and differentiate them. The results of FTIR and Raman spectroscopy revealed quantitative and qualitative changes in the nucleic acid and protein in the groups of cancer and atypical hyperplasia, in comparison with the control group. Changes in the lipid region were also observed in Raman spectra. Pearson correlation coefficient demonstrated a statistically significant correlation between Raman spectra for the cancer and atypical hyperplasia groups (0.747, <i>p</i> < 0.05) and for atypical hyperplasia and the controls (0.507, <i>p</i> < 0.05), while FTIR spectra demonstrated a statistically significant positive correlation for the same group as in Raman data and for the control and cancer groups (0.966, <i>p</i> < 0.05). To summarize, the method of spectroscopy enables differentiation of atypical hyperplasia and endometrial cancer tissues from the physiological endometrial tissue. |
| first_indexed | 2024-03-10T18:36:15Z |
| format | Article |
| id | doaj.art-19dcd6ad1f0049b3a30c567a1bdbe94d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:36:15Z |
| publishDate | 2020-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-19dcd6ad1f0049b3a30c567a1bdbe94d2023-11-20T06:10:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-07-012114482810.3390/ijms21144828Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and CancerEdyta Barnas0Joanna Skret-Magierlo1Andrzej Skret2Ewa Kaznowska3Joanna Depciuch4Kamil Szmuc5Kornelia Łach6Izabela Krawczyk-Marć7Jozef Cebulski8Institute of Health Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszów, PolandMedical College of Rzeszow University, Institute of Medical Sciences, Kopisto 2a, 35-959 Rzeszów, PolandMedical College of Rzeszow University, Institute of Medical Sciences, Kopisto 2a, 35-959 Rzeszów, PolandDepartment of Pathomorphology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, PolandInstitute of Nuclear Physics, Polish Academy of Science, Radzikowskiego 152, 31-342 Krakow, PolandInstitute of Physics, College of Natural Sciences, University of Rzeszow, - St. Pigonia 1, 35-310 Rzeszów, PolandClinic of Paediatric Oncology and Haematology, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, PolandDepartment of Human Histology, Chair of Morphological Sciences, College of Medical Sciences, University of Rzeszow, Kopisto 2a, 35-959 Rzeszow, PolandInstitute of Physics, College of Natural Sciences, University of Rzeszow, - St. Pigonia 1, 35-310 Rzeszów, PolandCurrently, endometrial carcinoma (EC) is the most common genital cancer in high-income countries. Some types of endometrial hyperplasia (EH) may be progressing to this malignancy. The diagnosis of EC and EH is based on time consuming histopathology evaluation, which is subjective and causes discrepancies in reassessment. Therefore, there is a need to create methods of objective evaluation allowing the diagnosis of early changes. The study aimed to simultaneously asses Fourier Transform Infrared (FTIR) and Raman spectroscopy combined with multidimensional analysis to identify the tissues of endometrial cancer, atypical hyperplasia and the normal control group, and differentiate them. The results of FTIR and Raman spectroscopy revealed quantitative and qualitative changes in the nucleic acid and protein in the groups of cancer and atypical hyperplasia, in comparison with the control group. Changes in the lipid region were also observed in Raman spectra. Pearson correlation coefficient demonstrated a statistically significant correlation between Raman spectra for the cancer and atypical hyperplasia groups (0.747, <i>p</i> < 0.05) and for atypical hyperplasia and the controls (0.507, <i>p</i> < 0.05), while FTIR spectra demonstrated a statistically significant positive correlation for the same group as in Raman data and for the control and cancer groups (0.966, <i>p</i> < 0.05). To summarize, the method of spectroscopy enables differentiation of atypical hyperplasia and endometrial cancer tissues from the physiological endometrial tissue.https://www.mdpi.com/1422-0067/21/14/4828endometrial canceratypical hyperplasiaFTIR spectroscopyRaman spectroscopycorrelation test |
| spellingShingle | Edyta Barnas Joanna Skret-Magierlo Andrzej Skret Ewa Kaznowska Joanna Depciuch Kamil Szmuc Kornelia Łach Izabela Krawczyk-Marć Jozef Cebulski Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer International Journal of Molecular Sciences endometrial cancer atypical hyperplasia FTIR spectroscopy Raman spectroscopy correlation test |
| title | Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer |
| title_full | Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer |
| title_fullStr | Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer |
| title_full_unstemmed | Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer |
| title_short | Simultaneous FTIR and Raman Spectroscopy in Endometrial Atypical Hyperplasia and Cancer |
| title_sort | simultaneous ftir and raman spectroscopy in endometrial atypical hyperplasia and cancer |
| topic | endometrial cancer atypical hyperplasia FTIR spectroscopy Raman spectroscopy correlation test |
| url | https://www.mdpi.com/1422-0067/21/14/4828 |
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