Microbiome composition and function within the Kellet’s whelk perivitelline fluid
ABSTRACTMicrobiomes have gained significant attention in ecological research, owing to their diverse interactions and essential roles within different organismal ecosystems. Microorganisms, such as bacteria, archaea, and viruses, have profound impact on host health, influencing digestion, metabolism...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-03-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.03514-23 |
| _version_ | 1797276722667716608 |
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| author | Benjamin N. Daniels Jenna Nurge Chanel De Smet Olivia Sleeper Crow White Jean M. Davidson Pat Fidopiastis |
| author_facet | Benjamin N. Daniels Jenna Nurge Chanel De Smet Olivia Sleeper Crow White Jean M. Davidson Pat Fidopiastis |
| author_sort | Benjamin N. Daniels |
| collection | DOAJ |
| description | ABSTRACTMicrobiomes have gained significant attention in ecological research, owing to their diverse interactions and essential roles within different organismal ecosystems. Microorganisms, such as bacteria, archaea, and viruses, have profound impact on host health, influencing digestion, metabolism, immune function, tissue development, and behavior. This study investigates the microbiome diversity and function of Kellet’s whelk (Kelletia kelletii) perivitelline fluid (PVF), which sustains thousands of developing K. kelletii embryos within a polysaccharide and protein matrix. Our core microbiome analysis reveals a diverse range of bacteria, with the Roseobacter genus being the most abundant. Additionally, genes related to host-microbe interactions, symbiosis, and quorum sensing were detected, indicating a potential symbiotic relationship between the microbiome and Kellet’s whelk embryos. Furthermore, the microbiome exhibits gene expression related to antibiotic biosynthesis, suggesting a defensive role against pathogenic bacteria and potential discovery of novel antibiotics. Overall, this study sheds light on the microbiome’s role in Kellet’s whelk development, emphasizing the significance of host-microbe interactions in vulnerable life history stages. To our knowledge, ours is the first study to use 16S sequencing coupled with RNA sequencing (RNA-seq) to profile the microbiome of an invertebrate PVF.IMPORTANCEThis study provides novel insight to an encapsulated system with strong evidence of symbiosis between the microbial inhabitants and developing host embryos. The Kellet’s whelk perivitelline fluid (PVF) contains microbial organisms of interest that may be providing symbiotic functions and potential antimicrobial properties during this vulnerable life history stage. This study, the first to utilize a comprehensive approach to investigating Kellet’s whelk PVF microbiome, couples 16S rRNA gene long-read sequencing with RNA-seq. This research contributes to and expands our knowledge on the roles of beneficial host-associated microbes. |
| first_indexed | 2024-03-07T15:33:22Z |
| format | Article |
| id | doaj.art-19ddea373c9b49508dc91a2df2486059 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-07T15:33:22Z |
| publishDate | 2024-03-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-19ddea373c9b49508dc91a2df24860592024-03-05T14:04:36ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-03-0112310.1128/spectrum.03514-23Microbiome composition and function within the Kellet’s whelk perivitelline fluidBenjamin N. Daniels0Jenna Nurge1Chanel De Smet2Olivia Sleeper3Crow White4Jean M. Davidson5Pat Fidopiastis6Department of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USADepartment of Biological Sciences, California Polytechnic State University, San Luis Obispo, California, USAABSTRACTMicrobiomes have gained significant attention in ecological research, owing to their diverse interactions and essential roles within different organismal ecosystems. Microorganisms, such as bacteria, archaea, and viruses, have profound impact on host health, influencing digestion, metabolism, immune function, tissue development, and behavior. This study investigates the microbiome diversity and function of Kellet’s whelk (Kelletia kelletii) perivitelline fluid (PVF), which sustains thousands of developing K. kelletii embryos within a polysaccharide and protein matrix. Our core microbiome analysis reveals a diverse range of bacteria, with the Roseobacter genus being the most abundant. Additionally, genes related to host-microbe interactions, symbiosis, and quorum sensing were detected, indicating a potential symbiotic relationship between the microbiome and Kellet’s whelk embryos. Furthermore, the microbiome exhibits gene expression related to antibiotic biosynthesis, suggesting a defensive role against pathogenic bacteria and potential discovery of novel antibiotics. Overall, this study sheds light on the microbiome’s role in Kellet’s whelk development, emphasizing the significance of host-microbe interactions in vulnerable life history stages. To our knowledge, ours is the first study to use 16S sequencing coupled with RNA sequencing (RNA-seq) to profile the microbiome of an invertebrate PVF.IMPORTANCEThis study provides novel insight to an encapsulated system with strong evidence of symbiosis between the microbial inhabitants and developing host embryos. The Kellet’s whelk perivitelline fluid (PVF) contains microbial organisms of interest that may be providing symbiotic functions and potential antimicrobial properties during this vulnerable life history stage. This study, the first to utilize a comprehensive approach to investigating Kellet’s whelk PVF microbiome, couples 16S rRNA gene long-read sequencing with RNA-seq. This research contributes to and expands our knowledge on the roles of beneficial host-associated microbes.https://journals.asm.org/doi/10.1128/spectrum.03514-23microbiomeRNA-seqantimicrobial activityantibiotic biosynthesisgene expressionGO term |
| spellingShingle | Benjamin N. Daniels Jenna Nurge Chanel De Smet Olivia Sleeper Crow White Jean M. Davidson Pat Fidopiastis Microbiome composition and function within the Kellet’s whelk perivitelline fluid Microbiology Spectrum microbiome RNA-seq antimicrobial activity antibiotic biosynthesis gene expression GO term |
| title | Microbiome composition and function within the Kellet’s whelk perivitelline fluid |
| title_full | Microbiome composition and function within the Kellet’s whelk perivitelline fluid |
| title_fullStr | Microbiome composition and function within the Kellet’s whelk perivitelline fluid |
| title_full_unstemmed | Microbiome composition and function within the Kellet’s whelk perivitelline fluid |
| title_short | Microbiome composition and function within the Kellet’s whelk perivitelline fluid |
| title_sort | microbiome composition and function within the kellet s whelk perivitelline fluid |
| topic | microbiome RNA-seq antimicrobial activity antibiotic biosynthesis gene expression GO term |
| url | https://journals.asm.org/doi/10.1128/spectrum.03514-23 |
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