Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine
Tobacco smoking continues to be a global epidemic and the leading preventable cause of cancer and cardiovascular disease. Nicotine vaccines have been investigated as an alternative to currently available smoking cessation strategies as a means to increase rates of success and long-term abstinence. R...
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MDPI AG
2021-02-01
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Online Access: | https://www.mdpi.com/2076-393X/9/2/118 |
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author | Nya L. Fraleigh Jordan D. Lewicky Alexandrine L. Martel Francisco Diaz-Mitoma Hoang-Thanh Le |
author_facet | Nya L. Fraleigh Jordan D. Lewicky Alexandrine L. Martel Francisco Diaz-Mitoma Hoang-Thanh Le |
author_sort | Nya L. Fraleigh |
collection | DOAJ |
description | Tobacco smoking continues to be a global epidemic and the leading preventable cause of cancer and cardiovascular disease. Nicotine vaccines have been investigated as an alternative to currently available smoking cessation strategies as a means to increase rates of success and long-term abstinence. Recently, we demonstrated that a mucosal nicotine vaccine was able to induce robust mucosal and systemic antibodies when delivered heterologously using intranasal and intramuscular routes. Herein, we investigated the neutralization ability of the anti-nicotine antibodies using both intranasal and intracardiac nicotine challenges. Combining the extraction of lyophilized organ samples with RP-HPLC methods, we were able to recover between 47% and 56% of the nicotine administered from the blood, brain, heart, and lungs up to 10 min after challenge, suggesting that the interaction of the antibodies with nicotine forms a stable complex independently of the route of vaccination or challenge. Although both challenge routes can be used for assessing systemic antibodies, only the intranasal administration of nicotine, which is more physiologically similar to the inhalation of nicotine, permitted the crucial interaction of nicotine with the mucosal antibodies generated using the heterologous vaccination route. Notably, these results were obtained 6 months after the final vaccination, demonstrating stable mucosal and systemic antibody responses. |
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issn | 2076-393X |
language | English |
last_indexed | 2024-03-09T05:57:58Z |
publishDate | 2021-02-01 |
publisher | MDPI AG |
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spelling | doaj.art-19ded2b61e2047dbbf45f99172fcad5e2023-12-03T12:11:16ZengMDPI AGVaccines2076-393X2021-02-019211810.3390/vaccines9020118Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine VaccineNya L. Fraleigh0Jordan D. Lewicky1Alexandrine L. Martel2Francisco Diaz-Mitoma3Hoang-Thanh Le4Department of Research, Health Sciences North Research Institute (HSNRI), 56 Walford Road, Sudbury, ON P3E 2H3, CanadaDepartment of Research, Health Sciences North Research Institute (HSNRI), 56 Walford Road, Sudbury, ON P3E 2H3, CanadaDepartment of Research, Health Sciences North Research Institute (HSNRI), 56 Walford Road, Sudbury, ON P3E 2H3, CanadaDepartment of Chemistry & Biochemistry, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, CanadaDepartment of Research, Health Sciences North Research Institute (HSNRI), 56 Walford Road, Sudbury, ON P3E 2H3, CanadaTobacco smoking continues to be a global epidemic and the leading preventable cause of cancer and cardiovascular disease. Nicotine vaccines have been investigated as an alternative to currently available smoking cessation strategies as a means to increase rates of success and long-term abstinence. Recently, we demonstrated that a mucosal nicotine vaccine was able to induce robust mucosal and systemic antibodies when delivered heterologously using intranasal and intramuscular routes. Herein, we investigated the neutralization ability of the anti-nicotine antibodies using both intranasal and intracardiac nicotine challenges. Combining the extraction of lyophilized organ samples with RP-HPLC methods, we were able to recover between 47% and 56% of the nicotine administered from the blood, brain, heart, and lungs up to 10 min after challenge, suggesting that the interaction of the antibodies with nicotine forms a stable complex independently of the route of vaccination or challenge. Although both challenge routes can be used for assessing systemic antibodies, only the intranasal administration of nicotine, which is more physiologically similar to the inhalation of nicotine, permitted the crucial interaction of nicotine with the mucosal antibodies generated using the heterologous vaccination route. Notably, these results were obtained 6 months after the final vaccination, demonstrating stable mucosal and systemic antibody responses.https://www.mdpi.com/2076-393X/9/2/118nicotine vaccinemucosalintranasalnicotine distributionnicotine analysis method |
spellingShingle | Nya L. Fraleigh Jordan D. Lewicky Alexandrine L. Martel Francisco Diaz-Mitoma Hoang-Thanh Le Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine Vaccines nicotine vaccine mucosal intranasal nicotine distribution nicotine analysis method |
title | Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine |
title_full | Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine |
title_fullStr | Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine |
title_full_unstemmed | Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine |
title_short | Assessing Neutralized Nicotine Distribution Using Mice Vaccinated with the Mucosal Conjugate Nicotine Vaccine |
title_sort | assessing neutralized nicotine distribution using mice vaccinated with the mucosal conjugate nicotine vaccine |
topic | nicotine vaccine mucosal intranasal nicotine distribution nicotine analysis method |
url | https://www.mdpi.com/2076-393X/9/2/118 |
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