Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1
Chitosan (CHI) and carboxymethyl cellulose (CMC) are naturally sourced materials with excellent physical, chemical, and biological properties, which make them a promising tool for the development of different medical devices. In this research, CHI-CMC wound dressings were manufactured, by using diff...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022015468 |
| _version_ | 1818028640735068160 |
|---|---|
| author | Jerónimo Osorio Echavarría Natalia Andrea Gómez Vanegas Claudia Patricia Ossa Orozco |
| author_facet | Jerónimo Osorio Echavarría Natalia Andrea Gómez Vanegas Claudia Patricia Ossa Orozco |
| author_sort | Jerónimo Osorio Echavarría |
| collection | DOAJ |
| description | Chitosan (CHI) and carboxymethyl cellulose (CMC) are naturally sourced materials with excellent physical, chemical, and biological properties, which make them a promising tool for the development of different medical devices. In this research, CHI-CMC wound dressings were manufactured, by using different colloidal suspensions of silver nanoparticles (AgNPs) synthesized from the ligninolytic fungus Anamorphous Bjerkandera sp. R1, called CS and SN. Transmission electron microscopy (TEM), UV-Vis spectroscopy, and dynamic light scattering (DLS) analysis were used to characterize AgNPs. The wound dressings were characterized, by scanning electron microscopy (SEM), optical microscopy and their mechanical, antimicrobial, and biological properties were evaluated. The results of the different characterizations revealed the formation of spherical AgNPs with a mean size between 10 and 70 nm for the different mixtures worked. The mechanical properties of CHI-CMS-AgNPs doped with CS and SN suspensions showed superior mechanical properties with respect to CHI-CMC wound dressings. Compared to the latter, CHI-CMC-AgNPs wound dressings yielded better antibacterial activity against the pathogen Escherichia coli. In biological assays, it was observed that manufactured CHI-CMC-AgNPs wound dressings were not toxic when in contact with human skin fibroblasts (Detroit). This study, then, suggests that this type of wound dressings with a chitosan matrix and carboxymethyl cellulose doped with biologically synthesized nanoparticles from the fungus Bjerkandera sp., may be an ideal alternative for the manufacture of new wound dressings. |
| first_indexed | 2024-12-10T05:07:01Z |
| format | Article |
| id | doaj.art-19e42a7c158e4a2eb7787d4d4c3f437a |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-12-10T05:07:01Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-19e42a7c158e4a2eb7787d4d4c3f437a2022-12-22T02:01:13ZengElsevierHeliyon2405-84402022-09-0189e10258Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1Jerónimo Osorio Echavarría0Natalia Andrea Gómez Vanegas1Claudia Patricia Ossa Orozco2Bioprocess Group, Department of Chemical Engineering, University of Antioquia, Street 70 # 52 – 21, Medellin 1226, Colombia; Corresponding author.Bioprocess Group, Department of Chemical Engineering, University of Antioquia, Street 70 # 52 – 21, Medellin 1226, ColombiaBiomaterials Research Group, Bioengineering Program, University of Antioquia, Street 70 # 52 – 21, Medellin 1226, ColombiaChitosan (CHI) and carboxymethyl cellulose (CMC) are naturally sourced materials with excellent physical, chemical, and biological properties, which make them a promising tool for the development of different medical devices. In this research, CHI-CMC wound dressings were manufactured, by using different colloidal suspensions of silver nanoparticles (AgNPs) synthesized from the ligninolytic fungus Anamorphous Bjerkandera sp. R1, called CS and SN. Transmission electron microscopy (TEM), UV-Vis spectroscopy, and dynamic light scattering (DLS) analysis were used to characterize AgNPs. The wound dressings were characterized, by scanning electron microscopy (SEM), optical microscopy and their mechanical, antimicrobial, and biological properties were evaluated. The results of the different characterizations revealed the formation of spherical AgNPs with a mean size between 10 and 70 nm for the different mixtures worked. The mechanical properties of CHI-CMS-AgNPs doped with CS and SN suspensions showed superior mechanical properties with respect to CHI-CMC wound dressings. Compared to the latter, CHI-CMC-AgNPs wound dressings yielded better antibacterial activity against the pathogen Escherichia coli. In biological assays, it was observed that manufactured CHI-CMC-AgNPs wound dressings were not toxic when in contact with human skin fibroblasts (Detroit). This study, then, suggests that this type of wound dressings with a chitosan matrix and carboxymethyl cellulose doped with biologically synthesized nanoparticles from the fungus Bjerkandera sp., may be an ideal alternative for the manufacture of new wound dressings.http://www.sciencedirect.com/science/article/pii/S2405844022015468BiosynthesisBiocompatibilityChitosan (CHI)Carboxymethyl Cellulose (CMC)Silver Nanoparticles (AgNPs)Anamorphous Bjerkandera sp. R1 |
| spellingShingle | Jerónimo Osorio Echavarría Natalia Andrea Gómez Vanegas Claudia Patricia Ossa Orozco Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 Heliyon Biosynthesis Biocompatibility Chitosan (CHI) Carboxymethyl Cellulose (CMC) Silver Nanoparticles (AgNPs) Anamorphous Bjerkandera sp. R1 |
| title | Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 |
| title_full | Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 |
| title_fullStr | Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 |
| title_full_unstemmed | Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 |
| title_short | Chitosan/carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus Anamorphous Bjerkandera sp. R1 |
| title_sort | chitosan carboxymethyl cellulose wound dressings supplemented with biologically synthesized silver nanoparticles from the ligninolytic fungus anamorphous bjerkandera sp r1 |
| topic | Biosynthesis Biocompatibility Chitosan (CHI) Carboxymethyl Cellulose (CMC) Silver Nanoparticles (AgNPs) Anamorphous Bjerkandera sp. R1 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022015468 |
| work_keys_str_mv | AT jeronimoosorioechavarria chitosancarboxymethylcellulosewounddressingssupplementedwithbiologicallysynthesizedsilvernanoparticlesfromtheligninolyticfungusanamorphousbjerkanderaspr1 AT nataliaandreagomezvanegas chitosancarboxymethylcellulosewounddressingssupplementedwithbiologicallysynthesizedsilvernanoparticlesfromtheligninolyticfungusanamorphousbjerkanderaspr1 AT claudiapatriciaossaorozco chitosancarboxymethylcellulosewounddressingssupplementedwithbiologicallysynthesizedsilvernanoparticlesfromtheligninolyticfungusanamorphousbjerkanderaspr1 |