Single-cell proteomics reveals changes in expression during hair-cell development
Hearing and balance rely on small sensory hair cells that reside in the inner ear. To explore dynamic changes in the abundant proteins present in differentiating hair cells, we used nanoliter-scale shotgun mass spectrometry of single cells, each ~1 picoliter, from utricles of embryonic day 15 chicke...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-11-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/50777 |
| _version_ | 1828376254215094272 |
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| author | Ying Zhu Mirko Scheibinger Daniel Christian Ellwanger Jocelyn F Krey Dongseok Choi Ryan T Kelly Stefan Heller Peter G Barr-Gillespie |
| author_facet | Ying Zhu Mirko Scheibinger Daniel Christian Ellwanger Jocelyn F Krey Dongseok Choi Ryan T Kelly Stefan Heller Peter G Barr-Gillespie |
| author_sort | Ying Zhu |
| collection | DOAJ |
| description | Hearing and balance rely on small sensory hair cells that reside in the inner ear. To explore dynamic changes in the abundant proteins present in differentiating hair cells, we used nanoliter-scale shotgun mass spectrometry of single cells, each ~1 picoliter, from utricles of embryonic day 15 chickens. We identified unique constellations of proteins or protein groups from presumptive hair cells and from progenitor cells. The single-cell proteomes enabled the de novo reconstruction of a developmental trajectory using protein expression levels, revealing proteins that greatly increased in expression during differentiation of hair cells (e.g., OCM, CRABP1, GPX2, AK1, GSTO1) and those that decreased during differentiation (e.g., TMSB4X, AGR3). Complementary single-cell transcriptome profiling showed corresponding changes in mRNA during maturation of hair cells. Single-cell proteomics data thus can be mined to reveal features of cellular development that may be missed with transcriptomics. |
| first_indexed | 2024-04-14T07:57:17Z |
| format | Article |
| id | doaj.art-19e6a3669263492e9fe408c93b8d336a |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:57:17Z |
| publishDate | 2019-11-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-19e6a3669263492e9fe408c93b8d336a2022-12-22T02:04:59ZengeLife Sciences Publications LtdeLife2050-084X2019-11-01810.7554/eLife.50777Single-cell proteomics reveals changes in expression during hair-cell developmentYing Zhu0Mirko Scheibinger1Daniel Christian Ellwanger2Jocelyn F Krey3Dongseok Choi4Ryan T Kelly5Stefan Heller6Peter G Barr-Gillespie7https://orcid.org/0000-0002-9787-5860Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, United StatesDepartment of Otolaryngology Head and Neck Surgery, Stanford University, Stanford, United StatesDepartment of Otolaryngology Head and Neck Surgery, Stanford University, Stanford, United States; Genome Analysis Unit, Amgen Research, Amgen Inc, South San Francisco, United StatesOregon Hearing Research Center, Oregon Health & Science University, Portland, United States; Vollum Institute, Oregon Health & Science University, Portland, United StatesOHSU-PSU School of Public Health, Oregon Health & Science University, Portland, United States; Graduate School of Dentistry, Kyung Hee University, Seoul, Republic of KoreaEnvironmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, United States; Department of Chemistry and Biochemistry, Brigham Young University, Provo, United StatesDepartment of Otolaryngology Head and Neck Surgery, Stanford University, Stanford, United StatesOregon Hearing Research Center, Oregon Health & Science University, Portland, United States; Vollum Institute, Oregon Health & Science University, Portland, United StatesHearing and balance rely on small sensory hair cells that reside in the inner ear. To explore dynamic changes in the abundant proteins present in differentiating hair cells, we used nanoliter-scale shotgun mass spectrometry of single cells, each ~1 picoliter, from utricles of embryonic day 15 chickens. We identified unique constellations of proteins or protein groups from presumptive hair cells and from progenitor cells. The single-cell proteomes enabled the de novo reconstruction of a developmental trajectory using protein expression levels, revealing proteins that greatly increased in expression during differentiation of hair cells (e.g., OCM, CRABP1, GPX2, AK1, GSTO1) and those that decreased during differentiation (e.g., TMSB4X, AGR3). Complementary single-cell transcriptome profiling showed corresponding changes in mRNA during maturation of hair cells. Single-cell proteomics data thus can be mined to reveal features of cellular development that may be missed with transcriptomics.https://elifesciences.org/articles/50777actinhair cellsmass spectrometrysingle cellsRNA sequencingdevelopment |
| spellingShingle | Ying Zhu Mirko Scheibinger Daniel Christian Ellwanger Jocelyn F Krey Dongseok Choi Ryan T Kelly Stefan Heller Peter G Barr-Gillespie Single-cell proteomics reveals changes in expression during hair-cell development eLife actin hair cells mass spectrometry single cells RNA sequencing development |
| title | Single-cell proteomics reveals changes in expression during hair-cell development |
| title_full | Single-cell proteomics reveals changes in expression during hair-cell development |
| title_fullStr | Single-cell proteomics reveals changes in expression during hair-cell development |
| title_full_unstemmed | Single-cell proteomics reveals changes in expression during hair-cell development |
| title_short | Single-cell proteomics reveals changes in expression during hair-cell development |
| title_sort | single cell proteomics reveals changes in expression during hair cell development |
| topic | actin hair cells mass spectrometry single cells RNA sequencing development |
| url | https://elifesciences.org/articles/50777 |
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