Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera
Abstract The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium caro...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02101-6 |
| _version_ | 1797822584181489664 |
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| author | Alia A. Shoeib Nader A. Ashmawy Ayman Kamal Sahar Abd El Fatah Zaki |
| author_facet | Alia A. Shoeib Nader A. Ashmawy Ayman Kamal Sahar Abd El Fatah Zaki |
| author_sort | Alia A. Shoeib |
| collection | DOAJ |
| description | Abstract The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA, decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm. Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn’t show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. It recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases, instead of copper pesticides. It could be a new, approach for disease management and increase the plant’s nutritional value. |
| first_indexed | 2024-03-13T10:11:15Z |
| format | Article |
| id | doaj.art-19ea836bc5bd4cacab3eba5d46f4d387 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-13T10:11:15Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-19ea836bc5bd4cacab3eba5d46f4d3872023-05-21T11:30:50ZengBMCMicrobial Cell Factories1475-28592023-05-0122111410.1186/s12934-023-02101-6Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg generaAlia A. Shoeib0Nader A. Ashmawy1Ayman Kamal2Sahar Abd El Fatah Zaki3Plant Pathology Department, Faculty of Agriculture, Alexandria UniversityPlant Pathology Department, Faculty of Agriculture, Alexandria UniversityPlant Pathology Department, Faculty of Agriculture, Alexandria UniversityEnvironmental Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, City of Scientific Research and Technological ApplicationsAbstract The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on bacterial DNA, proteins, and carbohydrates concentration of Pectobacterium carotovorum subsp. carotovorum, Enterobacter cloacae (soft rot), and Dickeya solani (soft rot/blackleg). Treated cells showed degradation in isolated DNA, decreased proteins and carbohydrates concentration compared with untreated cells. Using Scanning Electron Microscope (SEM), the treated cells showed collapsed and small pits in the cell wall. Using Transmission Electron Microscope (TEM), internal changes showed penetration of NPs inside the tested bacterial cells, the appearance of periplasmic space, formation of vacuoles, and condensation of cytoplasm. Disease severity ex vivo of potato tuber infected with tested genera demonstrated that NPs treatment didn’t show any rotted tissue compared with untreated. The ability to uptake and accumulate FeNPs from the soil in potato (Solanum tuberosum) seedlings; Inductively Coupled Plasma-Optical Emission Spectroscopy (ICP-OES) was used. It recorded an increase in iron content of treated potato (Solanum tuberosum) seedlings with NPs, compared with untreated. FeNPs can be used to control soft rot/blackleg diseases, instead of copper pesticides. It could be a new, approach for disease management and increase the plant’s nutritional value.https://doi.org/10.1186/s12934-023-02101-6Nano-MetalsBiomoleculesDickeya solaniProteinCarbohydrate |
| spellingShingle | Alia A. Shoeib Nader A. Ashmawy Ayman Kamal Sahar Abd El Fatah Zaki Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera Microbial Cell Factories Nano-Metals Biomolecules Dickeya solani Protein Carbohydrate |
| title | Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera |
| title_full | Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera |
| title_fullStr | Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera |
| title_full_unstemmed | Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera |
| title_short | Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera |
| title_sort | nano metals forming bacteria in egypt ii efficacy towards biomolecules ultrastructure growth parameters and eco friendly therapeutic of soft rot blackleg genera |
| topic | Nano-Metals Biomolecules Dickeya solani Protein Carbohydrate |
| url | https://doi.org/10.1186/s12934-023-02101-6 |
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