Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study
Introduction: Soft Tissue Tumours (STTs) are a heterogeneous group of lesions arising from non-epithelial extraskeletal tissue of the body. The use of Fine Needle Aspiration Cytology (FNAC) in the evaluation of STTs is debatable because of their extremely varied morphology. Aim: To assess the ef...
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| Format: | Article |
| Language: | English |
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JCDR Research and Publications Pvt. Ltd.
2021-07-01
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| Series: | National Journal of Laboratory Medicine |
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| Online Access: | http://www.njlm.net/articles/PDF/2489/48487_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
| _version_ | 1818883564815515648 |
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| author | Alaka Sahu Chitta Ranjan Prasad Anupa Toppo |
| author_facet | Alaka Sahu Chitta Ranjan Prasad Anupa Toppo |
| author_sort | Alaka Sahu |
| collection | DOAJ |
| description | Introduction: Soft Tissue Tumours (STTs) are a heterogeneous group
of lesions arising from non-epithelial extraskeletal tissue of the body.
The use of Fine Needle Aspiration Cytology (FNAC) in the evaluation
of STTs is debatable because of their extremely varied morphology.
Aim: To assess the efficacy of FNAC in the diagnosis of STTs as
a routine procedure.
Materials and Methods: The present study was a retrospective
study conducted at VSS Institute of Medical Science and
Research, Burla, Sambalpur, Odisha, India, from December 2016
to November 2018. A total of 186 cases of STT were correlated
with histopathological studies. Fine needle aspirate air-dried
smears were stained with Diff-Quik and 95% alcohol fixed smears
were stained with Papanicolaou (PAP) stain. Corresponding
histopathological sections were stained with Haematoxylin and
Eosin (H&E) stain. Immunostaining was performed as and when
required to confirm histological diagnosis. Results were analysed
using International Business Machines (IBM) Statistical Package
for the Social Sciences (SPSS) Statistics 22.0.
Results: Out of 186 cases, 162 (87.09%) were diagnosed as
benign and 24 (12.91%) as malignant. Three false negative and
two false positive cases were reported. The sensitivity, specificity,
Positive Predictive Values (PPV), Negative Predictive Values
(NPV) and overall diagnostic accuracy were 87.5%, 98.7%,
91.3%, 98.1% and 97.3%, respectively. There was statistically
significant difference between the cytological diagnosis and the
final histological diagnosis (χ2
=35.5, p<0.05).
Conclusion: Fine Needle Aspiration (FNA) is very important for
initial diagnostic work-up, while histopathology with the help of
immunostain provides a final diagnosis. |
| first_indexed | 2024-12-19T15:35:40Z |
| format | Article |
| id | doaj.art-19f24665b0ba4cbbba497b75320df6a8 |
| institution | Directory Open Access Journal |
| issn | 2277-8551 2455-6882 |
| language | English |
| last_indexed | 2024-12-19T15:35:40Z |
| publishDate | 2021-07-01 |
| publisher | JCDR Research and Publications Pvt. Ltd. |
| record_format | Article |
| series | National Journal of Laboratory Medicine |
| spelling | doaj.art-19f24665b0ba4cbbba497b75320df6a82022-12-21T20:15:36ZengJCDR Research and Publications Pvt. Ltd.National Journal of Laboratory Medicine2277-85512455-68822021-07-01103PO07PO1010.7860/NJLM/2021/48487:2489Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective StudyAlaka Sahu0Chitta Ranjan Prasad1Anupa Toppo2Associate Professor, Department of Pathology, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India.Associate Professor, Department of Transfusion Medicine, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India.Assistant Professor, Department of Pathology, Veer Surendra Sai Institute of Medical Sciences and Research, Burla, Odisha, India.Introduction: Soft Tissue Tumours (STTs) are a heterogeneous group of lesions arising from non-epithelial extraskeletal tissue of the body. The use of Fine Needle Aspiration Cytology (FNAC) in the evaluation of STTs is debatable because of their extremely varied morphology. Aim: To assess the efficacy of FNAC in the diagnosis of STTs as a routine procedure. Materials and Methods: The present study was a retrospective study conducted at VSS Institute of Medical Science and Research, Burla, Sambalpur, Odisha, India, from December 2016 to November 2018. A total of 186 cases of STT were correlated with histopathological studies. Fine needle aspirate air-dried smears were stained with Diff-Quik and 95% alcohol fixed smears were stained with Papanicolaou (PAP) stain. Corresponding histopathological sections were stained with Haematoxylin and Eosin (H&E) stain. Immunostaining was performed as and when required to confirm histological diagnosis. Results were analysed using International Business Machines (IBM) Statistical Package for the Social Sciences (SPSS) Statistics 22.0. Results: Out of 186 cases, 162 (87.09%) were diagnosed as benign and 24 (12.91%) as malignant. Three false negative and two false positive cases were reported. The sensitivity, specificity, Positive Predictive Values (PPV), Negative Predictive Values (NPV) and overall diagnostic accuracy were 87.5%, 98.7%, 91.3%, 98.1% and 97.3%, respectively. There was statistically significant difference between the cytological diagnosis and the final histological diagnosis (χ2 =35.5, p<0.05). Conclusion: Fine Needle Aspiration (FNA) is very important for initial diagnostic work-up, while histopathology with the help of immunostain provides a final diagnosis.http://www.njlm.net/articles/PDF/2489/48487_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(KM).pdfbenignimmunostainmalignant |
| spellingShingle | Alaka Sahu Chitta Ranjan Prasad Anupa Toppo Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study National Journal of Laboratory Medicine benign immunostain malignant |
| title | Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study |
| title_full | Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study |
| title_fullStr | Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study |
| title_full_unstemmed | Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study |
| title_short | Histocytopathological Evaluation of Soft Tissue Tumours: A Retrospective Study |
| title_sort | histocytopathological evaluation of soft tissue tumours a retrospective study |
| topic | benign immunostain malignant |
| url | http://www.njlm.net/articles/PDF/2489/48487_CE[Ra1]_F(SHU)_PF1(AG_SHU)_PFA(KM)_PB(AG_KM)_PN(KM).pdf |
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