Stable hepatitis C virus RNA detection by RT-PCR during four days storage
<p>Abstract</p> <p>Background</p> <p>Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2002-10-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/2/22 |
| _version_ | 1818644682206347264 |
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| author | Horsmans Yves Robert Annie Bodéus Monique de Moreau de Gerbehaye Anne-Isabelle Goubau Patrick |
| author_facet | Horsmans Yves Robert Annie Bodéus Monique de Moreau de Gerbehaye Anne-Isabelle Goubau Patrick |
| author_sort | Horsmans Yves |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples.</p> <p>Methods</p> <p>Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C) or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems).</p> <p>Results</p> <p>The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C).</p> <p>Conclusions</p> <p>We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.</p> |
| first_indexed | 2024-12-17T00:18:44Z |
| format | Article |
| id | doaj.art-19f674996c4c49188fb8ccc80943e097 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-17T00:18:44Z |
| publishDate | 2002-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-19f674996c4c49188fb8ccc80943e0972022-12-21T22:10:37ZengBMCBMC Infectious Diseases1471-23342002-10-01212210.1186/1471-2334-2-22Stable hepatitis C virus RNA detection by RT-PCR during four days storageHorsmans YvesRobert AnnieBodéus Moniquede Moreau de Gerbehaye Anne-IsabelleGoubau Patrick<p>Abstract</p> <p>Background</p> <p>Suboptimal specimen processing and storage conditions of samples which contain hepatitis C virus (HCV) RNA may result in a decline of HCV RNA concentration or false-negative results in the detection of HCV RNA in serum. We evaluated the stability of HCV RNA in serum and clotted blood samples stored at room temperature or at 4°C for 4 days with the aim of optimizing the standard procedures of processing and storage of samples.</p> <p>Methods</p> <p>Blood from five HCV RNA positive patients was collected in tubes with and without separator gel, centrifuged 1 or 6 hours after collection. Samples were then left 6, 24, 48, 72 or 96 h at room temperature (21.5 – 25.4°C) or at 4°C before determining their HCV RNA level using the COBAS AMPLICOR HCV MONITOR Test, vs 2.0 (Roche Diagnostic Systems).</p> <p>Results</p> <p>The logarithm of the HCV RNA level measurements remained within a 0.3 value of the means for 4 days at both temperatures (room temperature or 4°C).</p> <p>Conclusions</p> <p>We conclude that blood samples may be collected and aliquoted within 6 h of collection and can be stored at 4°C for 72 hours as proposed by the manufacturer without significant differences in measured HCV RNA level. Our results indicate that lapses in this scheme may still yield reliable results.</p>http://www.biomedcentral.com/1471-2334/2/22 |
| spellingShingle | Horsmans Yves Robert Annie Bodéus Monique de Moreau de Gerbehaye Anne-Isabelle Goubau Patrick Stable hepatitis C virus RNA detection by RT-PCR during four days storage BMC Infectious Diseases |
| title | Stable hepatitis C virus RNA detection by RT-PCR during four days storage |
| title_full | Stable hepatitis C virus RNA detection by RT-PCR during four days storage |
| title_fullStr | Stable hepatitis C virus RNA detection by RT-PCR during four days storage |
| title_full_unstemmed | Stable hepatitis C virus RNA detection by RT-PCR during four days storage |
| title_short | Stable hepatitis C virus RNA detection by RT-PCR during four days storage |
| title_sort | stable hepatitis c virus rna detection by rt pcr during four days storage |
| url | http://www.biomedcentral.com/1471-2334/2/22 |
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