Anti-Inflammatory and Antioxidant Activity of Acalypha hispida Leaf and Analysis of its Major Bioactive Polyphenols by HPLC
Purpose: Inflammation and oxidative stress can lead to different chronic diseases including cancer and atherosclerosis. Many medicinal plants have the potential to show as anti-inflammatory activity. Present investigation was performed to investigate anti-inflammatory, antioxidant activity, and quan...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2016-06-01
|
| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-6-275.pdf |
| Summary: | Purpose: Inflammation and oxidative stress can lead to
different chronic diseases including cancer and atherosclerosis. Many medicinal
plants have the potential to show as anti-inflammatory activity. Present
investigation was performed to investigate anti-inflammatory, antioxidant
activity, and quantification of selected bioactive plant polyphenols of the
ethanol (EAH) and aqueous (AAH) extracts of Acalypha
hispida (Euphorbiaceae) leaves.
Methods: Anti-inflammatory activity was evaluated by carragenan
and histamine induced rat paw edema models while antioxidant capacity was
evaluated by DPPH free radical scavenging, Fe+2 chelating ability,
reducing power, NO scavenging, total phenolic and total flavonoid content
assay. Identification and quantification of bioactive polyphenols was done by
HPLC.
Results: At the doses of 200 and 400 mg/kg, both EAH and AAH
showed statistically significant inhibition of paw volume in the
anti-inflammatory activity test. Both the extracts showed DPPH scavenging (IC50: 14 and 17 µg/ml,
respectively), Fe+2 ion chelating (IC50: 40 and 46 µg/ml,
respectively), NO scavenging activity (65.49 and 60.66% inhibition at 100
µg/ml), and concentration dependent reducing power ability. For EAH and
AAH, flavonoid content was 126.30 and
149.72 mg QE/g dry extract, while phenolic
content was 130.51 and 173.80 mg
GAE/g dry extract, respectively. HPLC analysis of EAH and AAH indicated the presence of high content of ellagic
acid along with other phenolic constituents.
Conclusion: High content of ellagic acid
along with other phenolic constituents might have played an important role in
the observed anti-inflammatory and antioxidant activity. |
|---|---|
| ISSN: | 2228-5881 2251-7308 |