Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production
One-fifth of the world’s water pollution has been generated by textile dyeing and fabric finishing process. Textile dyes affect the photosynthetic process and reduce the oxygen content in water leading to the death of aquatic plants. Industrial wastewater can be subjected to bioremediation by employ...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Cleaner Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772397622001095 |
| _version_ | 1828242637952385024 |
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| author | Mira chares Subash A.S. Maheshwari |
| author_facet | Mira chares Subash A.S. Maheshwari |
| author_sort | Mira chares Subash |
| collection | DOAJ |
| description | One-fifth of the world’s water pollution has been generated by textile dyeing and fabric finishing process. Textile dyes affect the photosynthetic process and reduce the oxygen content in water leading to the death of aquatic plants. Industrial wastewater can be subjected to bioremediation by employing efficient ligninolytic laccase enzymes. The laccases have gained popularity with efficient oxidation of recalcitrant environment pollutants. Similarly, microbial fuel cells can also be concerned with bioremediation treatment with atmospheric carbon dioxide reduction and production of potential energy sources. In this study, a new laccase-producing bacterium was isolated and identified as Ochrobacter pseudintermedium ASMCS06 by 16sr-RNA sequencing. Rice bran exhibited enhanced laccase activity compared to carbon and nitrogen sources. Laccase produced by O. pseudintermedium ASMCS06 was found to be stable at 75 °C and also at alkaline pH 8. Further, ASMCS06 was capable of degrading synthetic azo dye. After 72 h, the decolourization and degradation efficiencies of azo dye were 65 ± 2 % and 73 ± 1.8 %. Suggesting the possible degradation pathway, ASMSC06 was found to be potential with higher laccase activity and is suitable for dye degradation and other bioremediation applications. In addition, isolated bacteria also play a major role in bioelectricity production. The maximum voltage produced was identified as 415 mV on day 7. The isolated bacterium O. pseudintermedium ASMCS06 was able to produce a maximum current density of 930 (mA/cm2) and a maximum power density of 350 (mW/cm2). |
| first_indexed | 2024-04-12T22:18:09Z |
| format | Article |
| id | doaj.art-19f82cfe2d864331a0ea9393512d441f |
| institution | Directory Open Access Journal |
| issn | 2772-3976 |
| language | English |
| last_indexed | 2024-04-12T22:18:09Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cleaner Materials |
| spelling | doaj.art-19f82cfe2d864331a0ea9393512d441f2022-12-22T03:14:27ZengElsevierCleaner Materials2772-39762022-12-016100149Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity productionMira chares Subash0A.S. Maheshwari1Department of Chemical Engineering, National Institute of Technology, Tiruchirappalli, Tamil Nadu, India; Corresponding author.Department of Biotechnology, BIT-Campus, Anna University, Tiruchirappalli, Tamil Nadu, IndiaOne-fifth of the world’s water pollution has been generated by textile dyeing and fabric finishing process. Textile dyes affect the photosynthetic process and reduce the oxygen content in water leading to the death of aquatic plants. Industrial wastewater can be subjected to bioremediation by employing efficient ligninolytic laccase enzymes. The laccases have gained popularity with efficient oxidation of recalcitrant environment pollutants. Similarly, microbial fuel cells can also be concerned with bioremediation treatment with atmospheric carbon dioxide reduction and production of potential energy sources. In this study, a new laccase-producing bacterium was isolated and identified as Ochrobacter pseudintermedium ASMCS06 by 16sr-RNA sequencing. Rice bran exhibited enhanced laccase activity compared to carbon and nitrogen sources. Laccase produced by O. pseudintermedium ASMCS06 was found to be stable at 75 °C and also at alkaline pH 8. Further, ASMCS06 was capable of degrading synthetic azo dye. After 72 h, the decolourization and degradation efficiencies of azo dye were 65 ± 2 % and 73 ± 1.8 %. Suggesting the possible degradation pathway, ASMSC06 was found to be potential with higher laccase activity and is suitable for dye degradation and other bioremediation applications. In addition, isolated bacteria also play a major role in bioelectricity production. The maximum voltage produced was identified as 415 mV on day 7. The isolated bacterium O. pseudintermedium ASMCS06 was able to produce a maximum current density of 930 (mA/cm2) and a maximum power density of 350 (mW/cm2).http://www.sciencedirect.com/science/article/pii/S2772397622001095LaccaseO. pseudintermedium ASMCS06Dye-degradationBioelectricity |
| spellingShingle | Mira chares Subash A.S. Maheshwari Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production Cleaner Materials Laccase O. pseudintermedium ASMCS06 Dye-degradation Bioelectricity |
| title | Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production |
| title_full | Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production |
| title_fullStr | Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production |
| title_full_unstemmed | Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production |
| title_short | Investigation of Ochrobacter pseudintermedium ASMCS06 for Cleaner biodegradation and bioelectricity production |
| title_sort | investigation of ochrobacter pseudintermedium asmcs06 for cleaner biodegradation and bioelectricity production |
| topic | Laccase O. pseudintermedium ASMCS06 Dye-degradation Bioelectricity |
| url | http://www.sciencedirect.com/science/article/pii/S2772397622001095 |
| work_keys_str_mv | AT miracharessubash investigationofochrobacterpseudintermediumasmcs06forcleanerbiodegradationandbioelectricityproduction AT asmaheshwari investigationofochrobacterpseudintermediumasmcs06forcleanerbiodegradationandbioelectricityproduction |