Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity
<i>Euphorbia nivulia</i>-Ham (EN) is a neglected medicinal plant traditionally used for a number of pathologies, but it has not been explored scientifically. In the current study, its various fractions were assessed for their phenolic and flavonoid content, radical scavenging, as well as...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Life |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2075-1729/12/2/321 |
| _version_ | 1797478603067228160 |
|---|---|
| author | Muhammad Younus Muhammad Mohtasheem-ul-Hasan Shakeel Ijaz Muhammad Kamran Ambreen Maqsood Bushra Saddique Uzair Nisar Muhammad Ashraf Eman A. Mahmoud Ahmed M. El-Sabrout Hosam O. Elansary |
| author_facet | Muhammad Younus Muhammad Mohtasheem-ul-Hasan Shakeel Ijaz Muhammad Kamran Ambreen Maqsood Bushra Saddique Uzair Nisar Muhammad Ashraf Eman A. Mahmoud Ahmed M. El-Sabrout Hosam O. Elansary |
| author_sort | Muhammad Younus |
| collection | DOAJ |
| description | <i>Euphorbia nivulia</i>-Ham (EN) is a neglected medicinal plant traditionally used for a number of pathologies, but it has not been explored scientifically. In the current study, its various fractions were assessed for their phenolic and flavonoid content, radical scavenging, as well as its enzyme inhibitory potential. The hydro-alcoholic crude extract (ENCr) was subjected to a fractionation scheme to obtain different fractions, namely n-hexane (ENHF), chloroform (ENCF), n-butanol (ENBF), and aqueous fraction (ENAF). The obtained results revealed that the highest phenolic and flavonoid content, maximum radical scavenging potential (91 ± 0.55%), urease inhibition (54.36 ± 1.47%), and α-glucosidase inhibition (97.84 ± 1.87%) were exhibited by ENCr, while the ENBF fraction exhibited the highest acetylcholinestrase inhibition (57.32 ± 0.43%). Contrary to these, hydro-alcoholic crude as well as the other fractions showed no significant butyrylcholinestrases (BChE) and carbonic anhydrase inhibition activity. Conclusively, it was found that EN possesses a significant radical scavenging and enzyme inhibitory potential. Thus, the study may be regarded a step forward towards evidence-based phyto-medicine. |
| first_indexed | 2024-03-09T21:34:02Z |
| format | Article |
| id | doaj.art-19f84dda3a5c4b639747410f2698aa1a |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T21:34:02Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-19f84dda3a5c4b639747410f2698aa1a2023-11-23T20:47:29ZengMDPI AGLife2075-17292022-02-0112232110.3390/life12020321Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging ActivityMuhammad Younus0Muhammad Mohtasheem-ul-Hasan1Shakeel Ijaz2Muhammad Kamran3Ambreen Maqsood4Bushra Saddique5Uzair Nisar6Muhammad Ashraf7Eman A. Mahmoud8Ahmed M. El-Sabrout9Hosam O. Elansary10Department of Pharmacognosy, Faculty of Pharmacy, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmacognosy, Faculty of Pharmacy, University of Karachi, Sindh 75270, PakistanSchool of Pharmacy and Pharmaceutical Sciences, Trinity College Dublin, The University of Dublin, D02 PN40 Dublin, IrelandSchool of Agriculture, Food and Wine, The University of Adelaide, Adelaide 5005, AustraliaFaculty of Agriculture, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanFaculty of Agriculture, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Pharmacology, Faculty of Pharmacy, Ziauddin University, Karachi 75600, PakistanDepartment of Chemistry, The Islamia University of Bahawalpur, Bahawalpur 63100, PakistanDepartment of Food Industries, Faculty of Agriculture, Damietta University, Damietta 34511, EgyptDepartment of Applied Entomology and Zoology, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria 21545, EgyptPlant Production Department, College of Food & Agriculture Sciences, King Saud University, Riyadh 11451, Saudi Arabia<i>Euphorbia nivulia</i>-Ham (EN) is a neglected medicinal plant traditionally used for a number of pathologies, but it has not been explored scientifically. In the current study, its various fractions were assessed for their phenolic and flavonoid content, radical scavenging, as well as its enzyme inhibitory potential. The hydro-alcoholic crude extract (ENCr) was subjected to a fractionation scheme to obtain different fractions, namely n-hexane (ENHF), chloroform (ENCF), n-butanol (ENBF), and aqueous fraction (ENAF). The obtained results revealed that the highest phenolic and flavonoid content, maximum radical scavenging potential (91 ± 0.55%), urease inhibition (54.36 ± 1.47%), and α-glucosidase inhibition (97.84 ± 1.87%) were exhibited by ENCr, while the ENBF fraction exhibited the highest acetylcholinestrase inhibition (57.32 ± 0.43%). Contrary to these, hydro-alcoholic crude as well as the other fractions showed no significant butyrylcholinestrases (BChE) and carbonic anhydrase inhibition activity. Conclusively, it was found that EN possesses a significant radical scavenging and enzyme inhibitory potential. Thus, the study may be regarded a step forward towards evidence-based phyto-medicine.https://www.mdpi.com/2075-1729/12/2/321medicinal plantoxidative damageurease inhibitionα-glucosidase inhibitionacetylcholinestrase inhibition |
| spellingShingle | Muhammad Younus Muhammad Mohtasheem-ul-Hasan Shakeel Ijaz Muhammad Kamran Ambreen Maqsood Bushra Saddique Uzair Nisar Muhammad Ashraf Eman A. Mahmoud Ahmed M. El-Sabrout Hosam O. Elansary Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity Life medicinal plant oxidative damage urease inhibition α-glucosidase inhibition acetylcholinestrase inhibition |
| title | Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity |
| title_full | Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity |
| title_fullStr | Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity |
| title_full_unstemmed | Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity |
| title_short | Investigation of <em>Euphorbia nivulia</em>-HAM for Enzyme Inhibition Potential in Relation to the Phenolic and Flavonoid Contents and Radical Scavenging Activity |
| title_sort | investigation of em euphorbia nivulia em ham for enzyme inhibition potential in relation to the phenolic and flavonoid contents and radical scavenging activity |
| topic | medicinal plant oxidative damage urease inhibition α-glucosidase inhibition acetylcholinestrase inhibition |
| url | https://www.mdpi.com/2075-1729/12/2/321 |
| work_keys_str_mv | AT muhammadyounus investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT muhammadmohtasheemulhasan investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT shakeelijaz investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT muhammadkamran investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT ambreenmaqsood investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT bushrasaddique investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT uzairnisar investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT muhammadashraf investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT emanamahmoud investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT ahmedmelsabrout investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity AT hosamoelansary investigationofemeuphorbianivuliaemhamforenzymeinhibitionpotentialinrelationtothephenolicandflavonoidcontentsandradicalscavengingactivity |