Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)
Abstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese q...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-021-00271-7 |
| _version_ | 1797203211714560000 |
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| author | Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh |
| author_facet | Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh |
| author_sort | Dhafer A. Ali |
| collection | DOAJ |
| description | Abstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese quail (Coturnix japonica) using PCR-SSCP-sequencing, and to assess the possible association of the polymorphism of these regions with the main egg production traits. A total of 240 female birds with an equal number of three Japanese quail populations (Brown or BR, Black or BL, and White or WT) were included in this study. Results All the genotyped regions exerted no heterogeneity in their sequences with one exception detected in the exon 2. In this locus, a novel single nucleotide polymorphism (SNP) was detected in which “A” was substituted with “G” at 81 position with a silent effect (p.F79=SNP) on IGF2 protein. Association analyses indicated a significant (P < 0.05) relation of this SNP with egg number (EN) and bird weight (BW) in the analyzed populations, in which the birds with AG genotype had lower EN and BW than those with AA genotype. The p.F79=SNP was largely detected in the WT line than the other two lines. Conclusion The detected p.F79=SNP has a highly negative effect on EN and BW in Japanese quail. Thus, the implementation of the variations of the IGF2 gene can be a useful marker in the marker-assisted selection of Japanese quail. This is the first report to describe IGF2 gene variations in Japanese quail, which strongly suggests raising the birds from the BR line with AA genotype when breeders desire to raise Japanese quail for large-scale egg production. |
| first_indexed | 2024-04-24T08:15:44Z |
| format | Article |
| id | doaj.art-19f91cf240394bcaa39b41c40872b2bd |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:15:44Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-19f91cf240394bcaa39b41c40872b2bd2024-04-17T03:46:13ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202021-11-011911910.1186/s43141-021-00271-7Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica)Dhafer A. Ali0Mohammed Baqur S. Al-Shuhaib1Golzar Farhadi2Fadhil R. Al-Kafajy3Tahreer M. Al-Thuwaini4Ali Esmailizadeh5Department of Animal Production, College of Agriculture, Al-Qasim Green UniversityDepartment of Animal Production, College of Agriculture, Al-Qasim Green UniversityDepartment of Animal Science, Faculty of Agriculture, Shahid Bahonar University of KermanDepartment of Animal Production, College of Agriculture, Al-Qasim Green UniversityDepartment of Animal Production, College of Agriculture, Al-Qasim Green UniversityDepartment of Animal Science, Faculty of Agriculture, Shahid Bahonar University of KermanAbstract Background Insulin-like growth factor 2 (IGF2) is one of three hormones that share high structural similarity to insulin. It is involved in several insulin-like growth-regulating and mitogenic activities. This study was conducted to genotype the coding regions of the IGF2 gene in Japanese quail (Coturnix japonica) using PCR-SSCP-sequencing, and to assess the possible association of the polymorphism of these regions with the main egg production traits. A total of 240 female birds with an equal number of three Japanese quail populations (Brown or BR, Black or BL, and White or WT) were included in this study. Results All the genotyped regions exerted no heterogeneity in their sequences with one exception detected in the exon 2. In this locus, a novel single nucleotide polymorphism (SNP) was detected in which “A” was substituted with “G” at 81 position with a silent effect (p.F79=SNP) on IGF2 protein. Association analyses indicated a significant (P < 0.05) relation of this SNP with egg number (EN) and bird weight (BW) in the analyzed populations, in which the birds with AG genotype had lower EN and BW than those with AA genotype. The p.F79=SNP was largely detected in the WT line than the other two lines. Conclusion The detected p.F79=SNP has a highly negative effect on EN and BW in Japanese quail. Thus, the implementation of the variations of the IGF2 gene can be a useful marker in the marker-assisted selection of Japanese quail. This is the first report to describe IGF2 gene variations in Japanese quail, which strongly suggests raising the birds from the BR line with AA genotype when breeders desire to raise Japanese quail for large-scale egg production.https://doi.org/10.1186/s43141-021-00271-7Eggs productionGene variationIGF2Japanese quails |
| spellingShingle | Dhafer A. Ali Mohammed Baqur S. Al-Shuhaib Golzar Farhadi Fadhil R. Al-Kafajy Tahreer M. Al-Thuwaini Ali Esmailizadeh Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) Journal of Genetic Engineering and Biotechnology Eggs production Gene variation IGF2 Japanese quails |
| title | Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) |
| title_full | Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) |
| title_fullStr | Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) |
| title_full_unstemmed | Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) |
| title_short | Detection of a novel single nucleotide polymorphism in IGF2 gene with a negative impact on egg production and body weight in Japanese quail (Coturnix japonica) |
| title_sort | detection of a novel single nucleotide polymorphism in igf2 gene with a negative impact on egg production and body weight in japanese quail coturnix japonica |
| topic | Eggs production Gene variation IGF2 Japanese quails |
| url | https://doi.org/10.1186/s43141-021-00271-7 |
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