Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells
Lichens comprise a number of unique secondary metabolites with remarkable biological activities and have become an interesting research topic for cancer therapy. However, only a few of these metabolites have been assessed for their effectiveness against various in vitro models. Therefore, the aim of...
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MDPI AG
2021-03-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/3/420 |
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| author | Klaudia Petrova Martin Kello Tomas Kuruc Miriam Backorova Eva Petrovova Maria Vilkova Michal Goga Dajana Rucova Martin Backor Jan Mojzis |
| author_facet | Klaudia Petrova Martin Kello Tomas Kuruc Miriam Backorova Eva Petrovova Maria Vilkova Michal Goga Dajana Rucova Martin Backor Jan Mojzis |
| author_sort | Klaudia Petrova |
| collection | DOAJ |
| description | Lichens comprise a number of unique secondary metabolites with remarkable biological activities and have become an interesting research topic for cancer therapy. However, only a few of these metabolites have been assessed for their effectiveness against various in vitro models. Therefore, the aim of the present study was to assess the effect of extract <i>Pseudevernia furfuracea</i> (L.) Zopf (PSE) and its metabolite physodic acid (Phy) on tumour microenvironment (TME) modulation, focusing on epithelial–mesenchymal transition (EMT), cancer-associated fibroblasts (CAFs) transformation and angiogenesis. Here, we demonstrate, by using flow cytometry, Western blot and immunofluorescence microscopy, that tested compounds inhibited the EMT process in MCF-10A breast cells through decreasing the level of different mesenchymal markers in a time- and dose-dependent manner. By the same mechanisms, PSE and Phy suppressed the function of Transforming growth factor beta (TGF-β)-stimulated fibroblasts. Moreover, PSE and Phy resulted in a decreasing level of the TGF-β canonical pathway Smad2/3, which is essential for tumour growth. Furthermore, PSE and Phy inhibited angiogenesis <i>ex ovo</i> in a quail embryo chorioallantoic model, which indicates their potential anti-angiogenic activity. These results also provided the first evidence of the modulation of TME by these substances. |
| first_indexed | 2024-03-10T13:18:04Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T13:18:04Z |
| publishDate | 2021-03-01 |
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| series | Biomolecules |
| spelling | doaj.art-19fcd2b4d5cf46f98f4b57129b3e46f52023-11-21T10:13:40ZengMDPI AGBiomolecules2218-273X2021-03-0111342010.3390/biom11030420Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A CellsKlaudia Petrova0Martin Kello1Tomas Kuruc2Miriam Backorova3Eva Petrovova4Maria Vilkova5Michal Goga6Dajana Rucova7Martin Backor8Jan Mojzis9Department of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaDepartment of Pharmaceutical Technology, Pharmacognosy and Botany, University of Veterinary Medicine and Pharmacy, 041 81 Košice, SlovakiaDepartment of Anatomy, Histology and Physiology, University of Veterinary Medicine and Pharmacy, 041 81 Košice, SlovakiaDepartment of NMR Spectroscopy, Institute of Chemistry, Faculty of Science, Pavol Jozef Šafárik University, Moyzesova 11, 040 11 Košice, SlovakiaCore Facility Cell Imaging and Ultrastructure Research, University of Vienna, Althanstrasse 14, 1090 Vienna, AustriaDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Mánesova 23, 041 67 Košice, SlovakiaDepartment of Botany, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Mánesova 23, 041 67 Košice, SlovakiaDepartment of Pharmacology, Faculty of Medicine, Pavol Jozef Šafárik University, 040 01 Košice, SlovakiaLichens comprise a number of unique secondary metabolites with remarkable biological activities and have become an interesting research topic for cancer therapy. However, only a few of these metabolites have been assessed for their effectiveness against various in vitro models. Therefore, the aim of the present study was to assess the effect of extract <i>Pseudevernia furfuracea</i> (L.) Zopf (PSE) and its metabolite physodic acid (Phy) on tumour microenvironment (TME) modulation, focusing on epithelial–mesenchymal transition (EMT), cancer-associated fibroblasts (CAFs) transformation and angiogenesis. Here, we demonstrate, by using flow cytometry, Western blot and immunofluorescence microscopy, that tested compounds inhibited the EMT process in MCF-10A breast cells through decreasing the level of different mesenchymal markers in a time- and dose-dependent manner. By the same mechanisms, PSE and Phy suppressed the function of Transforming growth factor beta (TGF-β)-stimulated fibroblasts. Moreover, PSE and Phy resulted in a decreasing level of the TGF-β canonical pathway Smad2/3, which is essential for tumour growth. Furthermore, PSE and Phy inhibited angiogenesis <i>ex ovo</i> in a quail embryo chorioallantoic model, which indicates their potential anti-angiogenic activity. These results also provided the first evidence of the modulation of TME by these substances.https://www.mdpi.com/2218-273X/11/3/420tumour microenvironmentEMTMCF-10AfibroblastsHUVECsangiogenesis |
| spellingShingle | Klaudia Petrova Martin Kello Tomas Kuruc Miriam Backorova Eva Petrovova Maria Vilkova Michal Goga Dajana Rucova Martin Backor Jan Mojzis Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells Biomolecules tumour microenvironment EMT MCF-10A fibroblasts HUVECs angiogenesis |
| title | Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells |
| title_full | Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells |
| title_fullStr | Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells |
| title_full_unstemmed | Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells |
| title_short | Potential Effect of <i>Pseudevernia furfuracea</i> (L.) Zopf Extract and Metabolite Physodic Acid on Tumour Microenvironment Modulation in MCF-10A Cells |
| title_sort | potential effect of i pseudevernia furfuracea i l zopf extract and metabolite physodic acid on tumour microenvironment modulation in mcf 10a cells |
| topic | tumour microenvironment EMT MCF-10A fibroblasts HUVECs angiogenesis |
| url | https://www.mdpi.com/2218-273X/11/3/420 |
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