In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation
We demonstrated that arthritis could be visualized noninvasively using hydrophobically modified glycol chitosan nanoparticles labeled with Cy5.5 (HGC-Cy5.5) and an optical imaging system. Activated macrophages expressing Mac-1 molecules effectively phagocytosed HGC-Cy5.5, which formed spherical nano...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publications
2012-09-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2011.00056 |
| _version_ | 1797288211038339072 |
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| author | Kyeong Soon Park Jin Hee Kang Keum Hee Sa Hee Beom Koo Hyun Jung Cho Eon Jeong Nam In Chan Youn Kwang Meyung Kim In San Kim Ick Chan Kwon Kui Won Choi Young Mo Kang |
| author_facet | Kyeong Soon Park Jin Hee Kang Keum Hee Sa Hee Beom Koo Hyun Jung Cho Eon Jeong Nam In Chan Youn Kwang Meyung Kim In San Kim Ick Chan Kwon Kui Won Choi Young Mo Kang |
| author_sort | Kyeong Soon Park |
| collection | DOAJ |
| description | We demonstrated that arthritis could be visualized noninvasively using hydrophobically modified glycol chitosan nanoparticles labeled with Cy5.5 (HGC-Cy5.5) and an optical imaging system. Activated macrophages expressing Mac-1 molecules effectively phagocytosed HGC-Cy5.5, which formed spherical nanoparticles under physiologic conditions. We estimated the applicability of HGC-Cy5.5 to quantitative analysis of arthritis development and progression. Near-infrared fluorescence images, captured after HGC-Cy5.5 injection in mice with collagen-induced arthritis, showed stronger fluorescence intensity in the active arthritis group than in the nonarthritis group. According to the progression of arthritis in both collagen-induced arthritis and collagen antibody-induced arthritis models, total photon counts (TPCs) increased in parallel with the clinical arthritis index. Quantitative analysis of fluorescence after treatment with methotrexate showed a significant decrease in TPC in a dose-dependent manner. Histologic evaluation confirmed that the mechanism underlying selective accumulation of HGC-Cy5.5 within synovitis tissues included enhanced phagocytosis of the probe by Mac-1-expressing macrophages as well as enhanced permeability through leaky vessels. These results suggest that optical imaging of arthritis using HGC-Cy5.5 can provide an objective measurement of disease activity and, at the same time, therapeutic responses in rheumatoid arthritis. |
| first_indexed | 2024-03-07T18:46:04Z |
| format | Article |
| id | doaj.art-19ffc1a21c244a41b41a8ccb5d64ec92 |
| institution | Directory Open Access Journal |
| issn | 1536-0121 |
| language | English |
| last_indexed | 2024-03-07T18:46:04Z |
| publishDate | 2012-09-01 |
| publisher | SAGE Publications |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj.art-19ffc1a21c244a41b41a8ccb5d64ec922024-03-02T02:32:21ZengSAGE PublicationsMolecular Imaging1536-01212012-09-011110.2310/7290.2011.0005610.2310_7290.2011.00056In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive AccumulationKyeong Soon ParkJin Hee KangKeum Hee SaHee Beom KooHyun Jung ChoEon Jeong NamIn Chan YounKwang Meyung KimIn San KimIck Chan KwonKui Won ChoiYoung Mo KangWe demonstrated that arthritis could be visualized noninvasively using hydrophobically modified glycol chitosan nanoparticles labeled with Cy5.5 (HGC-Cy5.5) and an optical imaging system. Activated macrophages expressing Mac-1 molecules effectively phagocytosed HGC-Cy5.5, which formed spherical nanoparticles under physiologic conditions. We estimated the applicability of HGC-Cy5.5 to quantitative analysis of arthritis development and progression. Near-infrared fluorescence images, captured after HGC-Cy5.5 injection in mice with collagen-induced arthritis, showed stronger fluorescence intensity in the active arthritis group than in the nonarthritis group. According to the progression of arthritis in both collagen-induced arthritis and collagen antibody-induced arthritis models, total photon counts (TPCs) increased in parallel with the clinical arthritis index. Quantitative analysis of fluorescence after treatment with methotrexate showed a significant decrease in TPC in a dose-dependent manner. Histologic evaluation confirmed that the mechanism underlying selective accumulation of HGC-Cy5.5 within synovitis tissues included enhanced phagocytosis of the probe by Mac-1-expressing macrophages as well as enhanced permeability through leaky vessels. These results suggest that optical imaging of arthritis using HGC-Cy5.5 can provide an objective measurement of disease activity and, at the same time, therapeutic responses in rheumatoid arthritis.https://doi.org/10.2310/7290.2011.00056 |
| spellingShingle | Kyeong Soon Park Jin Hee Kang Keum Hee Sa Hee Beom Koo Hyun Jung Cho Eon Jeong Nam In Chan Youn Kwang Meyung Kim In San Kim Ick Chan Kwon Kui Won Choi Young Mo Kang In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation Molecular Imaging |
| title | In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation |
| title_full | In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation |
| title_fullStr | In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation |
| title_full_unstemmed | In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation |
| title_short | In Vivo Quantitative Measurement of Arthritis Activity Based on Hydrophobically Modified Glycol Chitosan in Inflammatory Arthritis: More Active than Passive Accumulation |
| title_sort | in vivo quantitative measurement of arthritis activity based on hydrophobically modified glycol chitosan in inflammatory arthritis more active than passive accumulation |
| url | https://doi.org/10.2310/7290.2011.00056 |
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