A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’
Lily is a popular flower worldwide due to its elegant appearance and pleasant fragrance. Floral volatiles of lily are predominated by monoterpenes and benzenoids. While a number of genes for monoterpene biosynthesis have been characterized, the molecular mechanism underlying floral benzenoid formati...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Plant Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1275960/full |
| _version_ | 1797670014114856960 |
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| author | Yuechong Yue Yuechong Yue Lan Wang Manyi Li Fang Liu Junle Yin Lijun Huang Bin Zhou Xinyue Li Xinyue Li Yunyi Yu Yunyi Yu Feng Chen Rangcai Yu Yanping Fan Yanping Fan |
| author_facet | Yuechong Yue Yuechong Yue Lan Wang Manyi Li Fang Liu Junle Yin Lijun Huang Bin Zhou Xinyue Li Xinyue Li Yunyi Yu Yunyi Yu Feng Chen Rangcai Yu Yanping Fan Yanping Fan |
| author_sort | Yuechong Yue |
| collection | DOAJ |
| description | Lily is a popular flower worldwide due to its elegant appearance and pleasant fragrance. Floral volatiles of lily are predominated by monoterpenes and benzenoids. While a number of genes for monoterpene biosynthesis have been characterized, the molecular mechanism underlying floral benzenoid formation in lily remains unclear. Here, we report on the identification and characterization of a novel BAHD acyltransferase gene that contributes to the biosynthesis of two related floral scent benzoate esters, ethyl benzoate and methyl benzoate, in the scented Lilium oriental hybrid ‘Siberia’. The emission of both methyl benzoate and ethyl benzoate in L. ‘Siberia’ was found to be tepal-specific, floral development-regulated and rhythmic. Through transcriptome profiling and bioinformatic analysis, a BAHD acyltransferase gene designated LoAAT1 was identified as the top candidate gene for the production of ethyl benzoate. In vitro enzyme assays and substrate feeding assays provide substantial evidence that LoAAT1 is responsible for the biosynthesis of ethyl benzoate. It was interesting to note that in in vitro enzyme assay, LoAAT1 can also catalyze the formation of methyl benzoate, which is typically formed by the action of benzoic acid methyltransferase (BAMT). The lack of an expressed putative BAMT gene in the flower transcriptome of L. ‘Siberia’, together with biochemical and expression evidence, led us to conclude that LoAAT1 is also responsible for, or at least contributes to, the biosynthesis of the floral scent compound methyl benzoate. This is the first report that a member of the plant BAHD acyltransferase family contributes to the production of both ethyl benzoate and methyl benzoate, presenting a new mechanism for the biosynthesis of benzoate esters. |
| first_indexed | 2024-03-11T20:53:22Z |
| format | Article |
| id | doaj.art-1a019193a5014f4bb99b78fc72e0a889 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-11T20:53:22Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-1a019193a5014f4bb99b78fc72e0a8892023-09-30T21:26:50ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-09-011410.3389/fpls.2023.12759601275960A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’Yuechong Yue0Yuechong Yue1Lan Wang2Manyi Li3Fang Liu4Junle Yin5Lijun Huang6Bin Zhou7Xinyue Li8Xinyue Li9Yunyi Yu10Yunyi Yu11Feng Chen12Rangcai Yu13Yanping Fan14Yanping Fan15The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaCollege of Life Sciences, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, ChinaDepartment of Plant Sciences, University of Tennessee, Knoxville, TN, United StatesCollege of Life Sciences, South China Agricultural University, Guangzhou, ChinaThe Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, ChinaGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, ChinaLily is a popular flower worldwide due to its elegant appearance and pleasant fragrance. Floral volatiles of lily are predominated by monoterpenes and benzenoids. While a number of genes for monoterpene biosynthesis have been characterized, the molecular mechanism underlying floral benzenoid formation in lily remains unclear. Here, we report on the identification and characterization of a novel BAHD acyltransferase gene that contributes to the biosynthesis of two related floral scent benzoate esters, ethyl benzoate and methyl benzoate, in the scented Lilium oriental hybrid ‘Siberia’. The emission of both methyl benzoate and ethyl benzoate in L. ‘Siberia’ was found to be tepal-specific, floral development-regulated and rhythmic. Through transcriptome profiling and bioinformatic analysis, a BAHD acyltransferase gene designated LoAAT1 was identified as the top candidate gene for the production of ethyl benzoate. In vitro enzyme assays and substrate feeding assays provide substantial evidence that LoAAT1 is responsible for the biosynthesis of ethyl benzoate. It was interesting to note that in in vitro enzyme assay, LoAAT1 can also catalyze the formation of methyl benzoate, which is typically formed by the action of benzoic acid methyltransferase (BAMT). The lack of an expressed putative BAMT gene in the flower transcriptome of L. ‘Siberia’, together with biochemical and expression evidence, led us to conclude that LoAAT1 is also responsible for, or at least contributes to, the biosynthesis of the floral scent compound methyl benzoate. This is the first report that a member of the plant BAHD acyltransferase family contributes to the production of both ethyl benzoate and methyl benzoate, presenting a new mechanism for the biosynthesis of benzoate esters.https://www.frontiersin.org/articles/10.3389/fpls.2023.1275960/fulllilyfloral scentBAHD acyltransferasemethyl benzoateethyl benzoatebiosynthesis |
| spellingShingle | Yuechong Yue Yuechong Yue Lan Wang Manyi Li Fang Liu Junle Yin Lijun Huang Bin Zhou Xinyue Li Xinyue Li Yunyi Yu Yunyi Yu Feng Chen Rangcai Yu Yanping Fan Yanping Fan A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ Frontiers in Plant Science lily floral scent BAHD acyltransferase methyl benzoate ethyl benzoate biosynthesis |
| title | A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ |
| title_full | A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ |
| title_fullStr | A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ |
| title_full_unstemmed | A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ |
| title_short | A BAHD acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of Lilium oriental hybrid ‘Siberia’ |
| title_sort | bahd acyltransferase contributes to the biosynthesis of both ethyl benzoate and methyl benzoate in the flowers of lilium oriental hybrid siberia |
| topic | lily floral scent BAHD acyltransferase methyl benzoate ethyl benzoate biosynthesis |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1275960/full |
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