Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome
Abstract Viruses are the most abundant components of the human gut microbiome with a significant impact on health and disease. The effects of human immunodeficiency virus (HIV) infection on gut virome has been scarcely analysed. Several studies suggested that integrase strand transfers inhibitors (I...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25979-5 |
| _version_ | 1828115149515390976 |
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| author | Pablo Villoslada-Blanco Patricia Pérez-Matute María Íñiguez Emma Recio-Fernández Daan Jansen Lander De Coninck Lila Close Pilar Blanco-Navarrete Luis Metola Valvanera Ibarra Jorge Alba Jelle Matthijnssens José A. Oteo |
| author_facet | Pablo Villoslada-Blanco Patricia Pérez-Matute María Íñiguez Emma Recio-Fernández Daan Jansen Lander De Coninck Lila Close Pilar Blanco-Navarrete Luis Metola Valvanera Ibarra Jorge Alba Jelle Matthijnssens José A. Oteo |
| author_sort | Pablo Villoslada-Blanco |
| collection | DOAJ |
| description | Abstract Viruses are the most abundant components of the human gut microbiome with a significant impact on health and disease. The effects of human immunodeficiency virus (HIV) infection on gut virome has been scarcely analysed. Several studies suggested that integrase strand transfers inhibitors (INSTIs) are associated with a healthier gut. Thus, the objective of this work was to evaluate the effects of HIV infection and INSTIs on gut virome composition. 26 non-HIV-infected volunteers, 15 naive HIV-infected patients and 15 INSTIs-treated HIV-infected patients were recruited and their gut virome composition was analysed using shotgun sequencing. Bacteriophages were the most abundant and diverse viruses present in gut. HIV infection was accompanied by a decrease in phage richness which was reverted after INSTIs-based treatment. β-diversity of phages revealed that samples from HIV-infected patients clustered separately from those belonging to the control group. Differential abundant analysis showed an increase in phages belonging to Caudoviricetes class in the naive group and a decrease of Malgrandaviricetes class phages in the INSTIs-treated group compared to the control group. Besides, it was observed that INSTIs-based treatment was not able to reverse the increase of lysogenic phages associated with HIV infection or to modify the decrease observed on the relative abundance of Proteobacteria-infecting phages. Our study describes for the first time the impact of HIV and INSTIs on gut virome and demonstrates that INSTIs-based treatments are able to partially restore gut dysbiosis at the viral level, which opens several opportunities for new studies focused on microbiota-based therapies. |
| first_indexed | 2024-04-11T12:36:21Z |
| format | Article |
| id | doaj.art-1a02ec931fc649e58f7b92812b09f317 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T12:36:21Z |
| publishDate | 2022-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a02ec931fc649e58f7b92812b09f3172022-12-22T04:23:36ZengNature PortfolioScientific Reports2045-23222022-12-0112111110.1038/s41598-022-25979-5Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut viromePablo Villoslada-Blanco0Patricia Pérez-Matute1María Íñiguez2Emma Recio-Fernández3Daan Jansen4Lander De Coninck5Lila Close6Pilar Blanco-Navarrete7Luis Metola8Valvanera Ibarra9Jorge Alba10Jelle Matthijnssens11José A. Oteo12Infectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR)Infectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR)Infectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR)Infectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR)Laboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU LeuvenLaboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU LeuvenLaboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU LeuvenCentro de Salud Siete Infantes de LaraInfectious Diseases Department, Hospital Universitario San PedroInfectious Diseases Department, Hospital Universitario San PedroInfectious Diseases Department, Hospital Universitario San PedroLaboratory of Viral Metagenomics, Department of Microbiology, Immunology and Transplantation, Rega Institute, KU LeuvenInfectious Diseases, Microbiota and Metabolism Unit, Infectious Diseases Department, Center for Biomedical Research of La Rioja (CIBIR)Abstract Viruses are the most abundant components of the human gut microbiome with a significant impact on health and disease. The effects of human immunodeficiency virus (HIV) infection on gut virome has been scarcely analysed. Several studies suggested that integrase strand transfers inhibitors (INSTIs) are associated with a healthier gut. Thus, the objective of this work was to evaluate the effects of HIV infection and INSTIs on gut virome composition. 26 non-HIV-infected volunteers, 15 naive HIV-infected patients and 15 INSTIs-treated HIV-infected patients were recruited and their gut virome composition was analysed using shotgun sequencing. Bacteriophages were the most abundant and diverse viruses present in gut. HIV infection was accompanied by a decrease in phage richness which was reverted after INSTIs-based treatment. β-diversity of phages revealed that samples from HIV-infected patients clustered separately from those belonging to the control group. Differential abundant analysis showed an increase in phages belonging to Caudoviricetes class in the naive group and a decrease of Malgrandaviricetes class phages in the INSTIs-treated group compared to the control group. Besides, it was observed that INSTIs-based treatment was not able to reverse the increase of lysogenic phages associated with HIV infection or to modify the decrease observed on the relative abundance of Proteobacteria-infecting phages. Our study describes for the first time the impact of HIV and INSTIs on gut virome and demonstrates that INSTIs-based treatments are able to partially restore gut dysbiosis at the viral level, which opens several opportunities for new studies focused on microbiota-based therapies.https://doi.org/10.1038/s41598-022-25979-5 |
| spellingShingle | Pablo Villoslada-Blanco Patricia Pérez-Matute María Íñiguez Emma Recio-Fernández Daan Jansen Lander De Coninck Lila Close Pilar Blanco-Navarrete Luis Metola Valvanera Ibarra Jorge Alba Jelle Matthijnssens José A. Oteo Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome Scientific Reports |
| title | Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome |
| title_full | Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome |
| title_fullStr | Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome |
| title_full_unstemmed | Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome |
| title_short | Impact of HIV infection and integrase strand transfer inhibitors-based treatment on the gut virome |
| title_sort | impact of hiv infection and integrase strand transfer inhibitors based treatment on the gut virome |
| url | https://doi.org/10.1038/s41598-022-25979-5 |
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