Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization
Single-molecule 3D tracking is critical to understand macromolecular dynamics but achieving this at a sub-millisecond resolution remains challenging. Here the authors present a 3D tracking method based on cross-entropy minimization and the true excitation point spread function.
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-36879-1 |
| _version_ | 1827983310097219584 |
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| author | Elias Amselem Bo Broadwater Tora Hävermark Magnus Johansson Johan Elf |
| author_facet | Elias Amselem Bo Broadwater Tora Hävermark Magnus Johansson Johan Elf |
| author_sort | Elias Amselem |
| collection | DOAJ |
| description | Single-molecule 3D tracking is critical to understand macromolecular dynamics but achieving this at a sub-millisecond resolution remains challenging. Here the authors present a 3D tracking method based on cross-entropy minimization and the true excitation point spread function. |
| first_indexed | 2024-04-09T22:46:40Z |
| format | Article |
| id | doaj.art-1a04ff4fc0d140b6a88699fe670fa71d |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-04-09T22:46:40Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-1a04ff4fc0d140b6a88699fe670fa71d2023-03-22T11:49:18ZengNature PortfolioNature Communications2041-17232023-03-0114111110.1038/s41467-023-36879-1Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimizationElias Amselem0Bo Broadwater1Tora Hävermark2Magnus Johansson3Johan Elf4Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala UniversityDepartment of Cell and Molecular Biology, Science for Life Laboratory, Uppsala UniversityDepartment of Cell and Molecular Biology, Science for Life Laboratory, Uppsala UniversityDepartment of Cell and Molecular Biology, Science for Life Laboratory, Uppsala UniversityDepartment of Cell and Molecular Biology, Science for Life Laboratory, Uppsala UniversitySingle-molecule 3D tracking is critical to understand macromolecular dynamics but achieving this at a sub-millisecond resolution remains challenging. Here the authors present a 3D tracking method based on cross-entropy minimization and the true excitation point spread function.https://doi.org/10.1038/s41467-023-36879-1 |
| spellingShingle | Elias Amselem Bo Broadwater Tora Hävermark Magnus Johansson Johan Elf Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization Nature Communications |
| title | Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization |
| title_full | Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization |
| title_fullStr | Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization |
| title_full_unstemmed | Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization |
| title_short | Real-time single-molecule 3D tracking in E. coli based on cross-entropy minimization |
| title_sort | real time single molecule 3d tracking in e coli based on cross entropy minimization |
| url | https://doi.org/10.1038/s41467-023-36879-1 |
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