Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study
Oxidative stress and antioxidants play an important role in obesity etiopathology. Genetic variants, including single nucleotide polymorphisms (SNPs) of the antioxidant-related genes, may impact disease risk in several populations. This preliminary study aimed to explore the association of 12 SNPs r...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/4/595 |
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| author | Amani M. T. Gusti Safaa Y. Qusti Eida M. Alshammari Eman A. Toraih Manal S. Fawzy |
| author_facet | Amani M. T. Gusti Safaa Y. Qusti Eida M. Alshammari Eman A. Toraih Manal S. Fawzy |
| author_sort | Amani M. T. Gusti |
| collection | DOAJ |
| description | Oxidative stress and antioxidants play an important role in obesity etiopathology. Genetic variants, including single nucleotide polymorphisms (SNPs) of the antioxidant-related genes, may impact disease risk in several populations. This preliminary study aimed to explore the association of 12 SNPs related to superoxide dismutase (<i>SOD</i>), catalase (<i>CAT</i>), glutathione peroxidase (<i>GPX</i>), glutathione-S-transferase (<i>GST</i>), and nitric oxide synthase (<i>NOS</i>) genes with obesity susceptibility in a Saudi population. A total of 384 unrelated participants, including 154 (40.1%) obese individuals, were enrolled. TaqMan OpenArray Genotyping assays were used. Six SNPs were significantly more prevalent in obese cohorts: (1) <i>GSTM1</i> rs1056806*C/T; (2) <i>SOD1</i> rs2234694*A; (3) <i>SOD2</i> rs4880*G; (4) <i>SOD3</i> rs2536512*A; (5) <i>GPX1</i> rs1800668*A; (6) <i>NOS3</i> rs1799983*G. Four SNPs were associated with higher obesity risk under heterozygote and dominant models for <i>GSTM1</i> rs1056806 (C/T), homozygote model for <i>SOD2</i> rs4880 (A/G), and homozygote and recessive models for <i>GPX1</i> rs1800668 (A/G). In contrast, <i>SOD3</i> rs2536512 (A/G) were less likely to be obese under heterozygote and dominant models. The CGAG, CAAA, TGGG, and CGAG combined genotypes showed a higher risk of obesity. In conclusion, the present results suggest that oxidative-stress-related genetic determinants could significantly associate with obesity risk in the study population. |
| first_indexed | 2024-03-10T12:22:52Z |
| format | Article |
| id | doaj.art-1a0622f5d23b44dc884395bc083fd680 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T12:22:52Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-1a0622f5d23b44dc884395bc083fd6802023-11-21T15:18:53ZengMDPI AGAntioxidants2076-39212021-04-0110459510.3390/antiox10040595Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control StudyAmani M. T. Gusti0Safaa Y. Qusti1Eida M. Alshammari2Eman A. Toraih3Manal S. Fawzy4Department of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Biochemistry, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi ArabiaDepartment of Chemistry, College of Science, University of Ha’il, Ha’il 2440, Saudi ArabiaDepartment of Surgery, School of Medicine, Tulane University, New Orleans, LA 70112, USADepartment of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Suez Canal University, Ismailia 41522, EgyptOxidative stress and antioxidants play an important role in obesity etiopathology. Genetic variants, including single nucleotide polymorphisms (SNPs) of the antioxidant-related genes, may impact disease risk in several populations. This preliminary study aimed to explore the association of 12 SNPs related to superoxide dismutase (<i>SOD</i>), catalase (<i>CAT</i>), glutathione peroxidase (<i>GPX</i>), glutathione-S-transferase (<i>GST</i>), and nitric oxide synthase (<i>NOS</i>) genes with obesity susceptibility in a Saudi population. A total of 384 unrelated participants, including 154 (40.1%) obese individuals, were enrolled. TaqMan OpenArray Genotyping assays were used. Six SNPs were significantly more prevalent in obese cohorts: (1) <i>GSTM1</i> rs1056806*C/T; (2) <i>SOD1</i> rs2234694*A; (3) <i>SOD2</i> rs4880*G; (4) <i>SOD3</i> rs2536512*A; (5) <i>GPX1</i> rs1800668*A; (6) <i>NOS3</i> rs1799983*G. Four SNPs were associated with higher obesity risk under heterozygote and dominant models for <i>GSTM1</i> rs1056806 (C/T), homozygote model for <i>SOD2</i> rs4880 (A/G), and homozygote and recessive models for <i>GPX1</i> rs1800668 (A/G). In contrast, <i>SOD3</i> rs2536512 (A/G) were less likely to be obese under heterozygote and dominant models. The CGAG, CAAA, TGGG, and CGAG combined genotypes showed a higher risk of obesity. In conclusion, the present results suggest that oxidative-stress-related genetic determinants could significantly associate with obesity risk in the study population.https://www.mdpi.com/2076-3921/10/4/595antioxidants-related genesCATGPXGSTNOSSOD |
| spellingShingle | Amani M. T. Gusti Safaa Y. Qusti Eida M. Alshammari Eman A. Toraih Manal S. Fawzy Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study Antioxidants antioxidants-related genes CAT GPX GST NOS SOD |
| title | Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study |
| title_full | Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study |
| title_fullStr | Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study |
| title_full_unstemmed | Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study |
| title_short | Antioxidants-Related Superoxide Dismutase (<i>SOD</i>), Catalase (<i>CAT</i>), Glutathione Peroxidase (<i>GPX</i>), Glutathione-S-Transferase (<i>GST</i>), and Nitric Oxide Synthase (<i>NOS</i>) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study |
| title_sort | antioxidants related superoxide dismutase i sod i catalase i cat i glutathione peroxidase i gpx i glutathione s transferase i gst i and nitric oxide synthase i nos i gene variants analysis in an obese population a preliminary case control study |
| topic | antioxidants-related genes CAT GPX GST NOS SOD |
| url | https://www.mdpi.com/2076-3921/10/4/595 |
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