Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts
Abstract Background Elevated CO2 partial pressure (pCO2) has been proposed as a potential steering parameter for selective carboxylate production in mixed culture fermentation. It is anticipated that intermediate product spectrum and production rates, as well as changes in the microbial community, a...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | Biotechnology for Biofuels and Bioproducts |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13068-023-02262-x |
| _version_ | 1797865307166998528 |
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| author | Pamela Ceron-Chafla Jo de Vrieze Korneel Rabaey Jules B. van Lier Ralph E. F. Lindeboom |
| author_facet | Pamela Ceron-Chafla Jo de Vrieze Korneel Rabaey Jules B. van Lier Ralph E. F. Lindeboom |
| author_sort | Pamela Ceron-Chafla |
| collection | DOAJ |
| description | Abstract Background Elevated CO2 partial pressure (pCO2) has been proposed as a potential steering parameter for selective carboxylate production in mixed culture fermentation. It is anticipated that intermediate product spectrum and production rates, as well as changes in the microbial community, are (in)directly influenced by elevated pCO2. However, it remains unclear how pCO2 interacts with other operational conditions, namely substrate specificity, substrate-to-biomass (S/X) ratio and the presence of an additional electron donor, and what effect pCO2 has on the exact composition of fermentation products. Here, we investigated possible steering effects of elevated pCO2 combined with (1) mixed substrate (glycerol/glucose) provision; (2) subsequent increments in substrate concentration to increase the S/X ratio; and (3) formate as an additional electron donor. Results Metabolite predominance, e.g., propionate vs. butyrate/acetate, and cell density, depended on interaction effects between pCO2–S/X ratio and pCO2–formate. Individual substrate consumption rates were negatively impacted by the interaction effect between pCO2–S/X ratio and were not re-established after lowering the S/X ratio and adding formate. The product spectrum was influenced by the microbial community composition, which in turn, was modified by substrate type and the interaction effect between pCO2–formate. High propionate and butyrate levels strongly correlated with Negativicutes and Clostridia predominance, respectively. After subsequent pressurized fermentation phases, the interaction effect between pCO2–formate enabled a shift from propionate towards succinate production when mixed substrate was provided. Conclusions Overall, interaction effects between elevated pCO2, substrate specificity, high S/X ratio and availability of reducing equivalents from formate, rather than an isolated pCO2 effect, modified the proportionality of propionate, butyrate and acetate in pressurized mixed substrate fermentations at the expense of reduced consumption rates and increased lag-phases. The interaction effect between elevated pCO2 and formate was beneficial for succinate production and biomass growth with a glycerol/glucose mixture as the substrate. The positive effect may be attributed to the availability of extra reducing equivalents, likely enhanced carbon fixating activity and hindered propionate conversion due to increased concentration of undissociated carboxylic acids. |
| first_indexed | 2024-04-09T23:05:59Z |
| format | Article |
| id | doaj.art-1a0816010f5b48d59892f4fcfa0aa376 |
| institution | Directory Open Access Journal |
| issn | 2731-3654 |
| language | English |
| last_indexed | 2024-04-09T23:05:59Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels and Bioproducts |
| spelling | doaj.art-1a0816010f5b48d59892f4fcfa0aa3762023-03-22T10:41:06ZengBMCBiotechnology for Biofuels and Bioproducts2731-36542023-02-0116112010.1186/s13068-023-02262-xSteering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery conceptsPamela Ceron-Chafla0Jo de Vrieze1Korneel Rabaey2Jules B. van Lier3Ralph E. F. Lindeboom4Sanitary Engineering Section, Department of Water Management, Delft University of TechnologyCenter for Microbial Ecology and Technology (CMET), Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Ghent UniversitySanitary Engineering Section, Department of Water Management, Delft University of TechnologySanitary Engineering Section, Department of Water Management, Delft University of TechnologyAbstract Background Elevated CO2 partial pressure (pCO2) has been proposed as a potential steering parameter for selective carboxylate production in mixed culture fermentation. It is anticipated that intermediate product spectrum and production rates, as well as changes in the microbial community, are (in)directly influenced by elevated pCO2. However, it remains unclear how pCO2 interacts with other operational conditions, namely substrate specificity, substrate-to-biomass (S/X) ratio and the presence of an additional electron donor, and what effect pCO2 has on the exact composition of fermentation products. Here, we investigated possible steering effects of elevated pCO2 combined with (1) mixed substrate (glycerol/glucose) provision; (2) subsequent increments in substrate concentration to increase the S/X ratio; and (3) formate as an additional electron donor. Results Metabolite predominance, e.g., propionate vs. butyrate/acetate, and cell density, depended on interaction effects between pCO2–S/X ratio and pCO2–formate. Individual substrate consumption rates were negatively impacted by the interaction effect between pCO2–S/X ratio and were not re-established after lowering the S/X ratio and adding formate. The product spectrum was influenced by the microbial community composition, which in turn, was modified by substrate type and the interaction effect between pCO2–formate. High propionate and butyrate levels strongly correlated with Negativicutes and Clostridia predominance, respectively. After subsequent pressurized fermentation phases, the interaction effect between pCO2–formate enabled a shift from propionate towards succinate production when mixed substrate was provided. Conclusions Overall, interaction effects between elevated pCO2, substrate specificity, high S/X ratio and availability of reducing equivalents from formate, rather than an isolated pCO2 effect, modified the proportionality of propionate, butyrate and acetate in pressurized mixed substrate fermentations at the expense of reduced consumption rates and increased lag-phases. The interaction effect between elevated pCO2 and formate was beneficial for succinate production and biomass growth with a glycerol/glucose mixture as the substrate. The positive effect may be attributed to the availability of extra reducing equivalents, likely enhanced carbon fixating activity and hindered propionate conversion due to increased concentration of undissociated carboxylic acids.https://doi.org/10.1186/s13068-023-02262-xElevated pCO2High-pressure carboxylates productionVeillonellaceaeSuccinate |
| spellingShingle | Pamela Ceron-Chafla Jo de Vrieze Korneel Rabaey Jules B. van Lier Ralph E. F. Lindeboom Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts Biotechnology for Biofuels and Bioproducts Elevated pCO2 High-pressure carboxylates production Veillonellaceae Succinate |
| title | Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts |
| title_full | Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts |
| title_fullStr | Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts |
| title_full_unstemmed | Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts |
| title_short | Steering the product spectrum in high-pressure anaerobic processes: CO2 partial pressure as a novel tool in biorefinery concepts |
| title_sort | steering the product spectrum in high pressure anaerobic processes co2 partial pressure as a novel tool in biorefinery concepts |
| topic | Elevated pCO2 High-pressure carboxylates production Veillonellaceae Succinate |
| url | https://doi.org/10.1186/s13068-023-02262-x |
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