Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata
Candida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little is known about its mechanisms for surviving acid stress in culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles in C. glabrata in the to...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-08-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00843/full |
| _version_ | 1818526359086956544 |
|---|---|
| author | Jing eWu Jing eWu Xiulai eChen Xiulai eChen Lijun eCai Lijun eCai Lei eTang Liming eLiu Liming eLiu |
| author_facet | Jing eWu Jing eWu Xiulai eChen Xiulai eChen Lijun eCai Lijun eCai Lei eTang Liming eLiu Liming eLiu |
| author_sort | Jing eWu |
| collection | DOAJ |
| description | Candida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little is known about its mechanisms for surviving acid stress in culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles in C. glabrata in the tolerance of acid stress, as the deletion of CgASG1 or CgHAL9 resulted in the inability to survive in an acidic environment. Cgasg1 and Cghal9 mutant strains are unable to maintain pH homeostasis, as evidenced by a decrease in intracellular pH and an increase in reactive oxygen species production, which results in metabolic disorders. The results showed that intracellular acidification was partly due to the diminished activity of the plasma membrane proton pump, CgPma1p. In addition, transcriptome sequencing revealed that Cgasg1 and Cghal9 mutant strains displayed a variety of changes in gene expression under acidic conditions, including genes in the MAPK signaling pathway, plasma membrane or cell wall organization, trehalose accumulation, and the RIM101 signaling pathway. Lastly, quantitative reverse-transcribed PCR and cellular localization showed that CgAsg1p and CgHal9p played independent roles in response to acid stress. |
| first_indexed | 2024-12-11T06:21:53Z |
| format | Article |
| id | doaj.art-1a095ce734bd43f5bc0a46812fd7f2e3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-11T06:21:53Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1a095ce734bd43f5bc0a46812fd7f2e32022-12-22T01:17:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-08-01610.3389/fmicb.2015.00843152965Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrataJing eWu0Jing eWu1Xiulai eChen2Xiulai eChen3Lijun eCai4Lijun eCai5Lei eTang6Liming eLiu7Liming eLiu8Jiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityCandida glabrata is an important microorganism used in commercial fermentation to produce pyruvate, but very little is known about its mechanisms for surviving acid stress in culture. In this study, it was shown that transcription factors Asg1p and Hal9p play essential roles in C. glabrata in the tolerance of acid stress, as the deletion of CgASG1 or CgHAL9 resulted in the inability to survive in an acidic environment. Cgasg1 and Cghal9 mutant strains are unable to maintain pH homeostasis, as evidenced by a decrease in intracellular pH and an increase in reactive oxygen species production, which results in metabolic disorders. The results showed that intracellular acidification was partly due to the diminished activity of the plasma membrane proton pump, CgPma1p. In addition, transcriptome sequencing revealed that Cgasg1 and Cghal9 mutant strains displayed a variety of changes in gene expression under acidic conditions, including genes in the MAPK signaling pathway, plasma membrane or cell wall organization, trehalose accumulation, and the RIM101 signaling pathway. Lastly, quantitative reverse-transcribed PCR and cellular localization showed that CgAsg1p and CgHal9p played independent roles in response to acid stress.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00843/fullCandida glabrataTranscription Factorsacid toleranceAsg1pHal9p |
| spellingShingle | Jing eWu Jing eWu Xiulai eChen Xiulai eChen Lijun eCai Lijun eCai Lei eTang Liming eLiu Liming eLiu Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata Frontiers in Microbiology Candida glabrata Transcription Factors acid tolerance Asg1p Hal9p |
| title | Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata |
| title_full | Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata |
| title_fullStr | Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata |
| title_full_unstemmed | Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata |
| title_short | Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata |
| title_sort | transcription factors asg1p and hal9p regulate ph homeostasis in candida glabrata |
| topic | Candida glabrata Transcription Factors acid tolerance Asg1p Hal9p |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00843/full |
| work_keys_str_mv | AT jingewu transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT jingewu transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT xiulaiechen transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT xiulaiechen transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT lijunecai transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT lijunecai transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT leietang transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT limingeliu transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata AT limingeliu transcriptionfactorsasg1pandhal9pregulatephhomeostasisincandidaglabrata |