Segmental Additive Tissue Engineering
Abstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenge...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2018-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-018-29270-4 |
| _version_ | 1818344061785866240 |
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| author | Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo |
| author_facet | Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo |
| author_sort | Martina Sladkova |
| collection | DOAJ |
| description | Abstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation. To address these issues, we have combined traditional and modular tissue engineering approaches in a strategy referred to as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a digital reconstruction of a rabbit femoral defect was partitioned transversally to the longitudinal axis into segments (modules) with discoidal geometry and defined thickness to enable protocol standardization and effective tissue formation in vitro. Bone grafts corresponding to each segment were then engineered using biomimetic scaffolds seeded with human induced pluripotent stem cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor with universal design. The SATE strategy enables the effective and reproducible engineering of segmental bone grafts for personalized skeletal reconstruction, and will facilitate technology transfer and implementation of a tissue engineering approach to segmental bone defect therapy. |
| first_indexed | 2024-12-13T16:40:30Z |
| format | Article |
| id | doaj.art-1a0af48e5e4f4ac2957fd8fbf2c31d58 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-13T16:40:30Z |
| publishDate | 2018-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1a0af48e5e4f4ac2957fd8fbf2c31d582022-12-21T23:38:18ZengNature PortfolioScientific Reports2045-23222018-07-018111110.1038/s41598-018-29270-4Segmental Additive Tissue EngineeringMartina Sladkova0Rawan Alawadhi1Rawan Jaragh Alhaddad2Asmaa Esmael3Shoug Alansari4Munerah Saad5Jenan Mulla Yousef6Lulwa Alqaoud7Giuseppe Maria de Peppo8The New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteThe New York Stem Cell Foundation Research InstituteAbstract Segmental bone defects caused by trauma and disease represent a major clinical problem worldwide. Current treatment options are limited and often associated with poor outcomes and severe complications. Bone engineering is a promising alternative solution, but a number of technical challenges must be addressed to allow for effective and reproducible construction of segmental grafts that meet the size and geometrical requirements needed for individual patients and routine clinical applications. It is important to devise engineering strategies and standard operating procedures that make it possible to scale up the size of bone-engineered grafts, minimize process and product variability, and facilitate technology transfer and implementation. To address these issues, we have combined traditional and modular tissue engineering approaches in a strategy referred to as Segmental Additive Tissue Engineering (SATE). To demonstrate this approach, a digital reconstruction of a rabbit femoral defect was partitioned transversally to the longitudinal axis into segments (modules) with discoidal geometry and defined thickness to enable protocol standardization and effective tissue formation in vitro. Bone grafts corresponding to each segment were then engineered using biomimetic scaffolds seeded with human induced pluripotent stem cell-derived mesodermal progenitors (iPSC-MPs) and a novel perfusion bioreactor with universal design. The SATE strategy enables the effective and reproducible engineering of segmental bone grafts for personalized skeletal reconstruction, and will facilitate technology transfer and implementation of a tissue engineering approach to segmental bone defect therapy.https://doi.org/10.1038/s41598-018-29270-4 |
| spellingShingle | Martina Sladkova Rawan Alawadhi Rawan Jaragh Alhaddad Asmaa Esmael Shoug Alansari Munerah Saad Jenan Mulla Yousef Lulwa Alqaoud Giuseppe Maria de Peppo Segmental Additive Tissue Engineering Scientific Reports |
| title | Segmental Additive Tissue Engineering |
| title_full | Segmental Additive Tissue Engineering |
| title_fullStr | Segmental Additive Tissue Engineering |
| title_full_unstemmed | Segmental Additive Tissue Engineering |
| title_short | Segmental Additive Tissue Engineering |
| title_sort | segmental additive tissue engineering |
| url | https://doi.org/10.1038/s41598-018-29270-4 |
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