Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study
High blood cholesterol levels are often associated with cardiovascular diseases. Therapeutic strategies, targeting different functions involved in cholesterol transport or synthesis, were developed to control cholesterolemia in human. However, the gut microbiota is also involved in cholesterol regul...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01121/full |
| _version_ | 1818290615651139584 |
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| author | Mélanie Bourgin Simon Labarthe Aicha Kriaa Marie Lhomme Marie Lhomme Philippe Gérard Philippe Lesnik Béatrice Laroche Emmanuelle Maguin Moez Rhimi |
| author_facet | Mélanie Bourgin Simon Labarthe Aicha Kriaa Marie Lhomme Marie Lhomme Philippe Gérard Philippe Lesnik Béatrice Laroche Emmanuelle Maguin Moez Rhimi |
| author_sort | Mélanie Bourgin |
| collection | DOAJ |
| description | High blood cholesterol levels are often associated with cardiovascular diseases. Therapeutic strategies, targeting different functions involved in cholesterol transport or synthesis, were developed to control cholesterolemia in human. However, the gut microbiota is also involved in cholesterol regulation by direct biotransformation of luminal cholesterol or conversion of bile salts, opening the way to the design of new strategies to manage cholesterol level. In this report, we developed for the first time a whole-body human model of cholesterol metabolism including the gut microbiota in order to investigate the relative impact of host and microbial pathways. We first used an animal model to investigate the ingested cholesterol distribution in vivo. Then, using in vitro bacterial growth experiments and metabolite measurements, we modeled the population dynamics of bacterial strains in the presence of cholesterol or bile salts, together with their bioconversion function. Next, after correct rescaling to mimic the activity of a complex microbiota, we developed a whole body model of cholesterol metabolism integrating host and microbiota mechanisms. This global model was validated with the animal experiments. Finally, the model was numerically explored to give a further insight into the different flux involved in cholesterol turn-over. According to this model, bacterial pathways appear as an important driver of cholesterol regulation, reinforcing the need for development of novel “bacteria-based” strategies for cholesterol management. |
| first_indexed | 2024-12-13T02:30:59Z |
| format | Article |
| id | doaj.art-1a0d50cfabe2410fba79adb91ef0e0c3 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T02:30:59Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1a0d50cfabe2410fba79adb91ef0e0c32022-12-22T00:02:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-06-011110.3389/fmicb.2020.01121525898Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical StudyMélanie Bourgin0Simon Labarthe1Aicha Kriaa2Marie Lhomme3Marie Lhomme4Philippe Gérard5Philippe Lesnik6Béatrice Laroche7Emmanuelle Maguin8Moez Rhimi9Micalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, FranceUniversité Paris-Saclay, INRAE, MaIAGE, Jouy-en-Josas, FranceMicalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, FranceINSERM, UMRS 1166, Sorbonne Universités, Hôpital Pitié-Salpétrière, Paris, FranceICANalytics, Institute of Cardiometabolism and Nutrition (IHU-ICAN, ANR-10-IAHU-05), Paris, FranceMicalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, FranceINSERM, UMRS 1166, Sorbonne Universités, Hôpital Pitié-Salpétrière, Paris, FranceUniversité Paris-Saclay, INRAE, MaIAGE, Jouy-en-Josas, FranceMicalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, FranceMicalis Institute, Université Paris-Saclay, INRAE, AgroParisTech, Jouy-en-Josas, FranceHigh blood cholesterol levels are often associated with cardiovascular diseases. Therapeutic strategies, targeting different functions involved in cholesterol transport or synthesis, were developed to control cholesterolemia in human. However, the gut microbiota is also involved in cholesterol regulation by direct biotransformation of luminal cholesterol or conversion of bile salts, opening the way to the design of new strategies to manage cholesterol level. In this report, we developed for the first time a whole-body human model of cholesterol metabolism including the gut microbiota in order to investigate the relative impact of host and microbial pathways. We first used an animal model to investigate the ingested cholesterol distribution in vivo. Then, using in vitro bacterial growth experiments and metabolite measurements, we modeled the population dynamics of bacterial strains in the presence of cholesterol or bile salts, together with their bioconversion function. Next, after correct rescaling to mimic the activity of a complex microbiota, we developed a whole body model of cholesterol metabolism integrating host and microbiota mechanisms. This global model was validated with the animal experiments. Finally, the model was numerically explored to give a further insight into the different flux involved in cholesterol turn-over. According to this model, bacterial pathways appear as an important driver of cholesterol regulation, reinforcing the need for development of novel “bacteria-based” strategies for cholesterol management.https://www.frontiersin.org/article/10.3389/fmicb.2020.01121/fullmicrobiotaholobiontmicrobiomefunctional ecologycholesterol metabolismwhole body model |
| spellingShingle | Mélanie Bourgin Simon Labarthe Aicha Kriaa Marie Lhomme Marie Lhomme Philippe Gérard Philippe Lesnik Béatrice Laroche Emmanuelle Maguin Moez Rhimi Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study Frontiers in Microbiology microbiota holobiont microbiome functional ecology cholesterol metabolism whole body model |
| title | Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study |
| title_full | Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study |
| title_fullStr | Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study |
| title_full_unstemmed | Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study |
| title_short | Exploring the Bacterial Impact on Cholesterol Cycle: A Numerical Study |
| title_sort | exploring the bacterial impact on cholesterol cycle a numerical study |
| topic | microbiota holobiont microbiome functional ecology cholesterol metabolism whole body model |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01121/full |
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