Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts
In recent years, new sources of secondary metabolites (SMs) in medicinal plants have been identified, and the introduction of these plants into field conditions has been carried out to obtain chemically diverse standardized raw material (RM). An example is mountain arnica <i>Arnica montana<...
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MDPI AG
2023-12-01
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| author | Piotr Sugier Joanna Jakubowicz-Gil Danuta Sugier Łukasz Sęczyk Adrian Zając Mateusz Pięt Roman Paduch |
| author_facet | Piotr Sugier Joanna Jakubowicz-Gil Danuta Sugier Łukasz Sęczyk Adrian Zając Mateusz Pięt Roman Paduch |
| author_sort | Piotr Sugier |
| collection | DOAJ |
| description | In recent years, new sources of secondary metabolites (SMs) in medicinal plants have been identified, and the introduction of these plants into field conditions has been carried out to obtain chemically diverse standardized raw material (RM). An example is mountain arnica <i>Arnica montana</i> L., one of Europe’s endemic endangered medicinal plant species, commonly used in pharmacy, cosmetics, and medicine. Its inflorescences (<i>Arnicae flos</i>) are characterized by anti-inflammatory, antiradical, antioxidant, antibacterial, antifungal, and antitumor properties. The main goals of the present research included: (i) characterization of the chemical composition of the inflorescences of <i>A. montana</i> harvested in different development stages; and (ii) presentation of the role of the development stage and different extraction methods in the antitumor activity of extracts through analyses of apoptosis, autophagy, and necrosis induction in human cervical carcinoma HeLa, human colon carcinoma HT29, and human colon metastatic carcinoma SW620 cell lines. The development stage was found to modify the composition of pharmacologically active substances, e.g., sesquiterpene lactones (SLs), flavonoids (Fs), and essential oil (EO), in arnica inflorescences. The content of Fs and EO increased during flowering to the full flowering phase; however, the highest content of SLs was noted in the full flowering phase and at the end of flowering. More promising results, i.e., a relatively high level of apoptosis and a low level of necrosis induced by the arnica extracts, were demonstrated in the HeLa cell line (full flowering; concentration: 0.5 µL/mL), the HT29 cell line (beginning of flowering; concentration: 0.5 µL/mL), and the SW620 cell line (stage of yellow buds; concentration: 1 µL/mL). This extremely valuable medicinal plant species provides a very broad range of RMs (e.g., inflorescences, rhizomes, roots, achenes, and all plant); therefore, attention should be paid to the more frequent use of water as a solvent in studies on the biological activity of mountain arnica extracts. |
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| language | English |
| last_indexed | 2024-03-08T21:02:15Z |
| publishDate | 2023-12-01 |
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| spelling | doaj.art-1a10b21ae075428290d064abdd5561902023-12-22T13:49:45ZengMDPI AGApplied Sciences2076-34172023-12-0113241297610.3390/app132412976Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence ExtractsPiotr Sugier0Joanna Jakubowicz-Gil1Danuta Sugier2Łukasz Sęczyk3Adrian Zając4Mateusz Pięt5Roman Paduch6Department of Botany, Mycology and Ecology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, PolandDepartment of Industrial and Medicinal Plants, University of Life Sciences in Lublin, 15 Akademicka Street, 20-950 Lublin, PolandDepartment of Industrial and Medicinal Plants, University of Life Sciences in Lublin, 15 Akademicka Street, 20-950 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Institute of Biological Sciences, Maria Curie-Skłodowska University, 19 Akademicka Street, 20-033 Lublin, PolandDepartment of Virology and Immunology, Institute of Biological Sciences, 19 Akademicka Street, 20-033 Lublin, PolandDepartment of Virology and Immunology, Institute of Biological Sciences, 19 Akademicka Street, 20-033 Lublin, PolandIn recent years, new sources of secondary metabolites (SMs) in medicinal plants have been identified, and the introduction of these plants into field conditions has been carried out to obtain chemically diverse standardized raw material (RM). An example is mountain arnica <i>Arnica montana</i> L., one of Europe’s endemic endangered medicinal plant species, commonly used in pharmacy, cosmetics, and medicine. Its inflorescences (<i>Arnicae flos</i>) are characterized by anti-inflammatory, antiradical, antioxidant, antibacterial, antifungal, and antitumor properties. The main goals of the present research included: (i) characterization of the chemical composition of the inflorescences of <i>A. montana</i> harvested in different development stages; and (ii) presentation of the role of the development stage and different extraction methods in the antitumor activity of extracts through analyses of apoptosis, autophagy, and necrosis induction in human cervical carcinoma HeLa, human colon carcinoma HT29, and human colon metastatic carcinoma SW620 cell lines. The development stage was found to modify the composition of pharmacologically active substances, e.g., sesquiterpene lactones (SLs), flavonoids (Fs), and essential oil (EO), in arnica inflorescences. The content of Fs and EO increased during flowering to the full flowering phase; however, the highest content of SLs was noted in the full flowering phase and at the end of flowering. More promising results, i.e., a relatively high level of apoptosis and a low level of necrosis induced by the arnica extracts, were demonstrated in the HeLa cell line (full flowering; concentration: 0.5 µL/mL), the HT29 cell line (beginning of flowering; concentration: 0.5 µL/mL), and the SW620 cell line (stage of yellow buds; concentration: 1 µL/mL). This extremely valuable medicinal plant species provides a very broad range of RMs (e.g., inflorescences, rhizomes, roots, achenes, and all plant); therefore, attention should be paid to the more frequent use of water as a solvent in studies on the biological activity of mountain arnica extracts.https://www.mdpi.com/2076-3417/13/24/12976<i>Arnica montana</i>development stageextraction methodsanticancer activity |
| spellingShingle | Piotr Sugier Joanna Jakubowicz-Gil Danuta Sugier Łukasz Sęczyk Adrian Zając Mateusz Pięt Roman Paduch Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts Applied Sciences <i>Arnica montana</i> development stage extraction methods anticancer activity |
| title | Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts |
| title_full | Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts |
| title_fullStr | Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts |
| title_full_unstemmed | Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts |
| title_short | Stages of Development and Solvents Determine the Anticancer Potential of Mountain Arnica (<i>Arnica montana</i> L.) Inflorescence Extracts |
| title_sort | stages of development and solvents determine the anticancer potential of mountain arnica i arnica montana i l inflorescence extracts |
| topic | <i>Arnica montana</i> development stage extraction methods anticancer activity |
| url | https://www.mdpi.com/2076-3417/13/24/12976 |
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