Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity.
Medicinal plants have been extensively studied since ancient times and exploited for their various therapeutic applications. These plants have a repository of numerous beneficial chemical constituents imputable to their possible bioactive metabolites. Throughout the ages, turmeric (Curcuma longa L.)...
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| Format: | Article |
| Language: | English |
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Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology
2022-08-01
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| Series: | Journal of Advanced Biotechnology and Experimental Therapeutics |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=8527 |
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| author | Himani Deepak Verinder Virk |
| author_facet | Himani Deepak Verinder Virk |
| author_sort | Himani Deepak |
| collection | DOAJ |
| description | Medicinal plants have been extensively studied since ancient times and exploited for their various therapeutic applications. These plants have a repository of numerous beneficial chemical constituents imputable to their possible bioactive metabolites. Throughout the ages, turmeric (Curcuma longa L.) has a renowned nutritional and therapeutic importance. Each part of turmeric like rhizome, roots, stem and leaves has its own medicinal properties. Inorder to recover endophytic mycoflora, epiphytic mycoflora must be eliminated via different sterilization techniques. In the present study, different sterilization methods have been assessed to isolate fungal endophytes from different parts of Curcuma longa L. The method involving the sequential washing of plant tissues with ethanol, sodium hypochlorite and ethanol used found more proficient in eliminating epiphytes. Five different surface sterilization treatments were evaluated for rhizome, roots, stem and leaves of turmeric plant. Moreover, treatment 5 with combination of 70% ethanol for 1 min., followed by 2% sodium chloride for 2 to 3 min (2 min for leaf, and stem; 3 min for roots and rhizome) and 70 % ethanol for 30 seconds was found effective. A total of 38 fungal endophytes were recovered and screened for antibacterial activity against human pathogens Staphylococcus aureus, Bacillus cereus, Pseudo¬monas aeruginosa and Escherichia coli. Out of 38 isolates, only 11 (28.9%) isolates were found effective in inhibiting either one or all test pathogens. The results confirmed that turmeric plant of Haridwar region represents an extremely rich reservoir of potential endophytic mycoflora with possible source of novel bioactive metabolites. Further studies will resolute the potential of endophytic fungi for various biological activities such as antioxidant, antidiabetic and immunomodulatory. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 334-346] |
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| format | Article |
| id | doaj.art-1a11e2b8d6104f95ac0bfb0d770502f4 |
| institution | Directory Open Access Journal |
| issn | 2616-4760 |
| language | English |
| last_indexed | 2024-12-12T03:03:21Z |
| publishDate | 2022-08-01 |
| publisher | Bangladesh Society for Microbiology, Immunology, and Advanced Biotechnology |
| record_format | Article |
| series | Journal of Advanced Biotechnology and Experimental Therapeutics |
| spelling | doaj.art-1a11e2b8d6104f95ac0bfb0d770502f42022-12-22T00:40:34ZengBangladesh Society for Microbiology, Immunology, and Advanced BiotechnologyJournal of Advanced Biotechnology and Experimental Therapeutics2616-47602022-08-015233434610.5455/jabet.2022.d1198527Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity.Himani Deepak0Verinder Virk1Research Scholar, Kanya Gurukula Campus GKV Haridwar Assistant Professor, Department of Microbiology, Kanya Gurukula Campus, Gurukula Kangari Deemed to be University, Haridwar, Uttarakhand, India-249407Medicinal plants have been extensively studied since ancient times and exploited for their various therapeutic applications. These plants have a repository of numerous beneficial chemical constituents imputable to their possible bioactive metabolites. Throughout the ages, turmeric (Curcuma longa L.) has a renowned nutritional and therapeutic importance. Each part of turmeric like rhizome, roots, stem and leaves has its own medicinal properties. Inorder to recover endophytic mycoflora, epiphytic mycoflora must be eliminated via different sterilization techniques. In the present study, different sterilization methods have been assessed to isolate fungal endophytes from different parts of Curcuma longa L. The method involving the sequential washing of plant tissues with ethanol, sodium hypochlorite and ethanol used found more proficient in eliminating epiphytes. Five different surface sterilization treatments were evaluated for rhizome, roots, stem and leaves of turmeric plant. Moreover, treatment 5 with combination of 70% ethanol for 1 min., followed by 2% sodium chloride for 2 to 3 min (2 min for leaf, and stem; 3 min for roots and rhizome) and 70 % ethanol for 30 seconds was found effective. A total of 38 fungal endophytes were recovered and screened for antibacterial activity against human pathogens Staphylococcus aureus, Bacillus cereus, Pseudo¬monas aeruginosa and Escherichia coli. Out of 38 isolates, only 11 (28.9%) isolates were found effective in inhibiting either one or all test pathogens. The results confirmed that turmeric plant of Haridwar region represents an extremely rich reservoir of potential endophytic mycoflora with possible source of novel bioactive metabolites. Further studies will resolute the potential of endophytic fungi for various biological activities such as antioxidant, antidiabetic and immunomodulatory. [ J Adv Biotechnol Exp Ther 2022; 5(2.000): 334-346]http://www.ejmanager.com/fulltextpdf.php?mno=8527curcuma longaendophytic fungisurface sterilizationantibacterial |
| spellingShingle | Himani Deepak Verinder Virk Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. Journal of Advanced Biotechnology and Experimental Therapeutics curcuma longa endophytic fungi surface sterilization antibacterial |
| title | Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. |
| title_full | Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. |
| title_fullStr | Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. |
| title_full_unstemmed | Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. |
| title_short | Optimization of surface sterilization method for the isolation of endophytic fungi associated with Curcuma longa L. and their antibacterial activity. |
| title_sort | optimization of surface sterilization method for the isolation of endophytic fungi associated with curcuma longa l and their antibacterial activity |
| topic | curcuma longa endophytic fungi surface sterilization antibacterial |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=8527 |
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