Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia
<i>Human orthopneumovirus</i> (HRSV) is a virus belonging to the <i>Pneumovirus</i> genus that causes lower respiratory tract infections (LRTI) in infants worldwide. In Tunisia, thousands of infants hospitalized for LRTI are found to be positive for HRSV but no whole genome s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/7/758 |
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| author | Valentina Curini Maurilia Marcacci Salma Abid Monia Ouederni Awatef ElMoussi Latifa Charaa Wafa Achour Ramzi Ouhichi Latifa Maazaoui Adriano Di Pasquale Hakim ElGhord Ahlem Gzara Alessandro Ripani Francesca Di Giallonardo Cesare Cammà Alessio Lorusso Ilhem Boutiba-Ben Boubaker |
| author_facet | Valentina Curini Maurilia Marcacci Salma Abid Monia Ouederni Awatef ElMoussi Latifa Charaa Wafa Achour Ramzi Ouhichi Latifa Maazaoui Adriano Di Pasquale Hakim ElGhord Ahlem Gzara Alessandro Ripani Francesca Di Giallonardo Cesare Cammà Alessio Lorusso Ilhem Boutiba-Ben Boubaker |
| author_sort | Valentina Curini |
| collection | DOAJ |
| description | <i>Human orthopneumovirus</i> (HRSV) is a virus belonging to the <i>Pneumovirus</i> genus that causes lower respiratory tract infections (LRTI) in infants worldwide. In Tunisia, thousands of infants hospitalized for LRTI are found to be positive for HRSV but no whole genome sequences of HRSV strains circulating in this country are available thus far. In this study, five nasal swab samples collected at different time points from a three-month-old female baby with severe immunodeficiency that was hospitalized for acute bronchiolitis were investigated by next generation sequencing. The Tunisian sequences from this study originated from samples collected in 2021, belong to the ON1 genotype of HRSV-A, and are clustered with European sequences from 2019 and not from 2020 or 2021. This is most likely related to local region-specific transmission of different HRSV-A variants due to the COVID-19 related travel restrictions. Overall, this is the first report describing the whole genome sequence of HRSV from Tunisia. However, more sequence data is needed to better understand the genetic diversity and transmission dynamic of HRSV. |
| first_indexed | 2024-03-09T13:13:12Z |
| format | Article |
| id | doaj.art-1a12ef55303645508000210ada146f57 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T13:13:12Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-1a12ef55303645508000210ada146f572023-11-30T21:39:15ZengMDPI AGPathogens2076-08172022-07-0111775810.3390/pathogens11070758Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in TunisiaValentina Curini0Maurilia Marcacci1Salma Abid2Monia Ouederni3Awatef ElMoussi4Latifa Charaa5Wafa Achour6Ramzi Ouhichi7Latifa Maazaoui8Adriano Di Pasquale9Hakim ElGhord10Ahlem Gzara11Alessandro Ripani12Francesca Di Giallonardo13Cesare Cammà14Alessio Lorusso15Ilhem Boutiba-Ben Boubaker16Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyLaboratory «Antimicrobial Resistance»(LR99ES09), Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, TunisiaDepartment of Peadiatrics: Immuno-Haematology and Stem Cell Transplantation, National Center of Bone Marrow Transplantation, Faculty of Medicine, Tunisia-University Tunis El Manar, Tunis 1006, TunisiaLaboratory «Antimicrobial Resistance»(LR99ES09), Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, TunisiaNational Influenza Center, Laboratory of Microbiology, Charles Nicolle Hospital, Tunis 1007, TunisiaLaboratory Department, National Center of Bone Marrow Transplantation, Tunis 1006, TunisiaTunisian Country WHO Office, Tunis 1003, TunisiaMinistry of Health, Primary Health Care Directorate, Tunis 1006, TunisiaIstituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyMinistry of Health, Primary Health Care Directorate, Tunis 1006, TunisiaMinistry of Health, Primary Health Care Directorate, Tunis 1006, TunisiaIstituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyThe Kirby Institute, University of New South Wales (UNSW), Sydney, NSW 2052, AustraliaIstituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyIstituto Zooprofilattico Sperimentale dell’Abruzzo e Molise (IZS-Teramo), 64100 Teramo, ItalyLaboratory «Antimicrobial Resistance»(LR99ES09), Faculty of Medicine of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia<i>Human orthopneumovirus</i> (HRSV) is a virus belonging to the <i>Pneumovirus</i> genus that causes lower respiratory tract infections (LRTI) in infants worldwide. In Tunisia, thousands of infants hospitalized for LRTI are found to be positive for HRSV but no whole genome sequences of HRSV strains circulating in this country are available thus far. In this study, five nasal swab samples collected at different time points from a three-month-old female baby with severe immunodeficiency that was hospitalized for acute bronchiolitis were investigated by next generation sequencing. The Tunisian sequences from this study originated from samples collected in 2021, belong to the ON1 genotype of HRSV-A, and are clustered with European sequences from 2019 and not from 2020 or 2021. This is most likely related to local region-specific transmission of different HRSV-A variants due to the COVID-19 related travel restrictions. Overall, this is the first report describing the whole genome sequence of HRSV from Tunisia. However, more sequence data is needed to better understand the genetic diversity and transmission dynamic of HRSV.https://www.mdpi.com/2076-0817/11/7/758<i>Human orthopneumovirus</i>next generation sequencingwhole genomephylogenetic analysisHRSV-A |
| spellingShingle | Valentina Curini Maurilia Marcacci Salma Abid Monia Ouederni Awatef ElMoussi Latifa Charaa Wafa Achour Ramzi Ouhichi Latifa Maazaoui Adriano Di Pasquale Hakim ElGhord Ahlem Gzara Alessandro Ripani Francesca Di Giallonardo Cesare Cammà Alessio Lorusso Ilhem Boutiba-Ben Boubaker Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia Pathogens <i>Human orthopneumovirus</i> next generation sequencing whole genome phylogenetic analysis HRSV-A |
| title | Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia |
| title_full | Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia |
| title_fullStr | Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia |
| title_full_unstemmed | Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia |
| title_short | Full Genome Characterization of Respiratory Syncytial Virus Causing a Fatal Infection in an Immunocompromised Patient in Tunisia |
| title_sort | full genome characterization of respiratory syncytial virus causing a fatal infection in an immunocompromised patient in tunisia |
| topic | <i>Human orthopneumovirus</i> next generation sequencing whole genome phylogenetic analysis HRSV-A |
| url | https://www.mdpi.com/2076-0817/11/7/758 |
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